Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation DOI Open Access
Elizabeth C. Marin, Billy J Morris, Tomke Stürner

и другие.

Опубликована: Июль 22, 2024

Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Язык: Английский

Circuit modules linking internal states and social behaviour in flies and mice DOI
David J. Anderson

Nature reviews. Neuroscience, Год журнала: 2016, Номер 17(11), С. 692 - 704

Опубликована: Окт. 18, 2016

Язык: Английский

Процитировано

310
NBLAST: Rapid, Sensitive Comparison of Neuronal Structure and Construction of Neuron Family Databases DOI Creative Commons
Marta Costa, James D. Manton, Aaron D. Ostrovsky

и другие.

Neuron, Год журнала: 2016, Номер 91(2), С. 293 - 311

Опубликована: Июль 1, 2016

Neural circuit mapping is generating datasets of tens thousands labeled neurons. New computational tools are needed to search and organize these data. We present NBLAST, a sensitive rapid algorithm, for measuring pairwise neuronal similarity. NBLAST considers both position local geometry, decomposing neurons into short segments; matched segments scored using probabilistic scoring matrix defined by statistics matches non-matches. validated on published dataset 16,129 single Drosophila can distinguish types down the finest level (single identified neurons) without priori information. Cluster analysis extensively studied classes new unreported topographical features. Fully automated clustering organized validation 1,052 clusters, many which map onto previously described types. supports additional query types, including searching against transgene expression patterns. Finally, we show that effective with data from other invertebrates zebrafish.Video AbstracteyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiJhY2I5ZGYyYjM4NDU3YmM1YTUzYzdkYjNmNjEwZTQ4ZiIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjc4MTcxMDE4fQ.esxS0ekj1Y2KrsvFNwLvBdO2uH7jTmSFdQuhvlKJHyudrp2pKJEX-13uDWQ0Vb-JtD0U9SwqzpnV2DKOlPJvu29jUXE06S3vSh1rpOHE7yqVyktsslvNDCTAC9dV_z1qUYL5DGzpgAxr_zWEnxssrGPSboU8WmBMtoEOD5p6_YUaw9I62XinRlM7U8lo0lTExmdwYGzd5e9RzdbxAl5X6uTJOqnjVEFEfw9qJnokYGLy9vHYQEzzmtU6zKKKq4FSXrZE-iprVz0KxAHTxxYlvTNb_xsfwA4twiDr5oq6vAmeX6SWLOruwCJx91IIIeKvrS8GHJZSnb9ca5CAPHmtng(mp4, (15.82 MB) Download video

Язык: Английский

Процитировано

307
Evolution of a central neural circuit underlies Drosophila mate preferences DOI
Laura F. Seeholzer,

Max Seppo,

David L. Stern

и другие.

Nature, Год журнала: 2018, Номер 559(7715), С. 564 - 569

Опубликована: Июль 1, 2018

Язык: Английский

Процитировано

223
P1 interneurons promote a persistent internal state that enhances inter-male aggression in Drosophila DOI Creative Commons
Eric D. Hoopfer,

Yonil Jung,

H. Inagaki

и другие.

eLife, Год журнала: 2015, Номер 4

Опубликована: Дек. 29, 2015

How brains are hardwired to produce aggressive behavior, and how aggression circuits related those that mediate courtship, is not well understood. A large-scale screen for aggression-promoting neurons in Drosophila identified several independent hits enhanced both inter-male courtship. Genetic intersections revealed 8-10 P1 interneurons, previously thought exclusively control male were sufficient promote fighting. Optogenetic experiments indicated activation could at a threshold below required wing extension. the absence of extension triggered persistent via an internal state endure minutes. High-frequency promoted suppressed during photostimulation, whereas resumed was inhibited following photostimulation offset. Thus, neuron promotes latent, facilitates controls overt expression these social behaviors threshold-dependent, inverse manner.

Язык: Английский

Процитировано

210
Gene regulation by gonadal hormone receptors underlies brain sex differences DOI Creative Commons
Bruno Gegenhuber, Melody V. Wu, Robert Bronstein

и другие.

Nature, Год журнала: 2022, Номер 606(7912), С. 153 - 159

Опубликована: Май 4, 2022

Abstract Oestradiol establishes neural sex differences in many vertebrates 1–3 and modulates mood, behaviour energy balance adulthood 4–8 . In the canonical pathway, oestradiol exerts its effects through transcription factor oestrogen receptor-α (ERα) 9 Although ERα has been extensively characterized breast cancer, neuronal targets of ERα, their involvement brain differences, remain largely unknown. Here we generate a comprehensive map genomic ERα-binding sites sexually dimorphic circuit that mediates social behaviours. We conclude orchestrates sexual differentiation mouse two mechanisms: establishing male-biased neuron types activating sustained gene expression program. Collectively, our findings reveal are defined by hormonal activation steroid receptors. The molecular identify may underlie on development, disease.

Язык: Английский

Процитировано

141
Sexual arousal gates visual processing during Drosophila courtship DOI
Tom Hindmarsh Sten, Rufei Li, Adriane G. Otopalik

и другие.

Nature, Год журнала: 2021, Номер 595(7868), С. 549 - 553

Опубликована: Июль 7, 2021

Язык: Английский

Процитировано

108
Nested neural circuits generate distinct acoustic signals during Drosophila courtship DOI Creative Commons
Joshua L. Lillvis, Kaiyu Wang, Hiroshi Shiozaki

и другие.

Current Biology, Год журнала: 2024, Номер 34(4), С. 808 - 824.e6

Опубликована: Янв. 30, 2024

Many motor control systems generate multiple movements using a common set of muscles. How are premotor circuits able to flexibly diverse movement patterns? Here, we characterize the neuronal that drive distinct courtship songs Drosophila melanogaster. Male flies vibrate their wings toward females produce two different song modes—pulse and sine song—which signal species identity male quality. Using cell-type-specific genetic reagents connectome, provide cellular synaptic map in ventral nerve cord these examine how activating or inhibiting each cell type within affects song. Our data reveal circuit is organized into nested feedforward pathways with extensive reciprocal feedback connections. The larger network produces pulse song, more complex ancestral form. A subset this simpler recent Such organization may be feature which evolution has layered increasing flexibility onto basic pattern.

Язык: Английский

Процитировано

21
Sexual circuitry in Drosophila DOI
Thomas O. Auer, Richard Benton

Current Opinion in Neurobiology, Год журнала: 2016, Номер 38, С. 18 - 26

Опубликована: Март 3, 2016

Язык: Английский

Процитировано

157
A complex peripheral code for salt taste in Drosophila DOI Creative Commons

Alexandria Jaeger,

Molly Stanley, Zachary F. Weiss

и другие.

eLife, Год журнала: 2018, Номер 7

Опубликована: Окт. 11, 2018

Each taste modality is generally encoded by a single, molecularly defined, population of sensory cells. However, salt stimulates multiple pathways in mammals and insects, suggesting more complex code for taste. Here, we examine coding Drosophila. After creating comprehensive molecular map comprised five discrete neuron classes across the fly labellum, find that four are activated salt: two exhibiting characteristics ‘low salt’ cells, ‘high classes. Behaviorally, low attraction depends primarily on ‘sweet’ neurons, with additional input from neurons expressing ionotropic receptor IR94e. High avoidance mediated ‘bitter’ glutamatergic Ppk23. Interestingly, impact these prior consumption. These results support model flies combinatorially integrates inputs cell types to afford robust flexible behaviors.

Язык: Английский

Процитировано

149
Visual Projection Neurons Mediating Directed Courtship in Drosophila DOI Creative Commons
Inês Ribeiro,

Michael Drews,

Armin Bahl

и другие.

Cell, Год журнала: 2018, Номер 174(3), С. 607 - 621.e18

Опубликована: Июль 1, 2018

Язык: Английский

Процитировано

145