Recent progress in xenotransplantation and its application to pediatric kidney disease
Pediatric Nephrology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 30, 2025
Язык: Английский
A chronological history of heart valve prostheses to offer perspectives of their limitations
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Фев. 14, 2025
Prosthetic
heart
valves
(PHV)
have
been
studied
for
around
70
years.
They
are
the
best
alternative
to
save
life
of
patients
with
cardiac
valve
diseases.
However,
current
PHVs
may
still
cause
significant
disadvantages
patients.
In
general,
native
show
complex
structures
and
reproducing
their
functions
challenges
scientists.
Valve
repair
replacement
options
heal
diseases
(VHDs),
such
as
stenosis
regurgitation,
which
high
morbidity
mortality
worldwide.
contributes
performance
cycles.
it
fails
restore
anatomy
its
normal
condition.
On
other
hand,
is
only
treat
degeneration.
It
do
so
by
mechanical
or
bioprosthetic
valves.
Although
prostheses
restructure
patients’
cycle,
both
limitations
potential
disadvantages,
causing
thrombogenicity
valves,
calcification.
Thus,
require
constant
improvements
remedy
these
limitations.
design
has
improved,
raw
materials
great
alternatives
this
problem
remain
scarce.
Cardiac
tissue
engineering
emerged
30
years
ago
improved
over
time,
e.g.,
xenografts
fabricated
serving
scaffolds
cell
seeding.
review
describes
substitutes,
starting
history
valvular
prosthesis
transplants
ending
some
perspectives
alleviate
artificial
Язык: Английский
In Situ Biomimetic Glycocalyx Layer Protects Endothelial Damage in Xenotransplantation
Nano Letters,
Год журнала:
2025,
Номер
25(11), С. 4576 - 4585
Опубликована: Март 10, 2025
Xenotransplantation
offers
a
transformative
solution
to
the
global
organ
shortage
crisis.
However,
survival
of
xenografts
remains
limited
despite
various
proposed
strategies.
In
this
study,
we
present
an
endothelial
cell
protection
strategy
that
extends
graft
through
in
situ
construction
biomimetic
glycan-enriched
nanofibers.
These
molecules
specifically
target
integrin
αvβ3
and
form
polysaccharide-structured
nanofiber
network
on
vascular
surface.
This
protects
cells
without
compromising
their
normal
physiological
functions.
The
constructed
layer
significantly
increased
xenograft
by
1.64-fold
compared
untreated
groups.
work
introduces
novel
enhance
heart
xenografts.
Язык: Английский
Pre-clinical xenotransplantation: physiology and pharmacy in human decedent and non-human primate models
Frontiers in Transplantation,
Год журнала:
2025,
Номер
4
Опубликована: Апрель 17, 2025
Non-human
primates
and
decedent
humans
have
emerged
as
the
two
principal
translational
models
in
xenotransplantation.
Each
model
has
differing
advantages
drawbacks.
In
this
manuscript,
we
will
compare
contrast
relative
strengths
of
each
model,
focusing
on
physiologic
function
xenograft
a
human
or
non-human
primate.
Additionally,
discuss
pharmacologic
agents
typically
employed
highlighting
both
ability
to
test
clinically-relevant
medication
strategies
that
may
be
impossible
due
species-specificity.
Язык: Английский
Application of Methods Detecting Xenotransplantation-Relevant Viruses for Screening German Slaughterhouse Pigs
Viruses,
Год журнала:
2024,
Номер
16(7), С. 1119 - 1119
Опубликована: Июль 11, 2024
Detection
methods
have
been
developed
to
prevent
transmission
of
zoonotic
or
xenozoonotic
porcine
viruses
after
transplantation
pig
organs
cells
the
recipient
(xenotransplantation).
Eleven
xenotransplantation-relevant
viruses,
including
cytomegalovirus,
roseolovirus
(PCMV/PRV),
lymphotropic
herpesviruses
-1,
-2,
-3
(PLHV-1,
2,
3),
parvovirus
(PPV),
circovirus
3,
4
(PCV2,
4),
hepatitis
E
virus
genotype
3
(HEV3),
endogenous
retrovirus-C
(PERV-C),
and
recombinant
PERV-A/C
selected.
In
past,
several
breeds,
minipigs,
genetically
modified
pigs
generated
for
xenotransplantation
had
analyzed
using
these
methods.
Here,
spleen,
liver,
blood
samples
from
10
German
slaughterhouse
were
screened
both
PCR-based
immunological
assays.
Five
viruses:
PCMV/PRV,
PLHV-1,
PLHV-3,
PERV-C,
found
in
all
animals,
PCV3
one
animal.
Some
animals
latently
infected
with
as
only
virus-specific
antibodies
detected.
Others
also
PCR
positive
spleen
and/or
indicative
an
ongoing
infection.
These
results
provide
important
information
on
that
infect
pigs,
together
previous
studies,
they
reveal
test
strategies
efficiently
work
under
field
conditions.
Язык: Английский
Application of Methods Detecting Xenotransplantation-Relevant Viruses for Screening German Slaughterhouse Pigs
Опубликована: Июнь 24, 2024
Detection
methods
have
been
developed
to
prevent
transmission
of
zoonotic
or
xenozoonotic
porcine
viruses
after
transplantation
pig
organs
cells
the
recipient
(xenotransplantation).
Eleven
xenotransplantation-relevant
including
cytomegalovirus,
a
roseolovirus
(PCMV/PRV),
lymphotropic
herpesviruses
-1,
-2,
-3
(PLHV-1,
2,
3),
parvovirus
(PPV),
circovirus
3,
4
(PCV2,
4),
hepatitis
E
virus
genotype
3
(HEV3),
endogenous
retrovirus-C
(PERV-C)
and
recombinant
PERV-A/C
selected.
In
past,
several
breeds,
minipigs
genetically
modified
pigs
generated
for
xenotransplantation
had
analyzed
using
these
methods.
Here,
spleen,
liver
blood
samples
from
10
German
slaughterhouse
were
screened
both,
PCR-based
immunological
assays.
Five
viruses:
PCMV/PRV,
PLHV-1,
PLHV-3
PERV-C
found
in
all
animals,
PCV3
one
animal.
Some
animals
latently
infected
with
PCMV/PRV
as
only
virus-specific
antibodies
detected.
Others
also
PCR
positive
spleen
and/or
liver,
indicative
an
ongoing
infection.
These
results
provide
important
information
on
that
infect
together
previous
studies
revealed
test
strategies
efficiently
work
under
field
conditions.
Язык: Английский
Revolutionizing Donor Heart Procurement: Innovations and Future Directions for Enhanced Transplantation Outcomes
Journal of Cardiovascular Development and Disease,
Год журнала:
2024,
Номер
11(8), С. 235 - 235
Опубликована: Июль 27, 2024
Heart
failure
persists
as
a
critical
public
health
challenge,
with
heart
transplantation
esteemed
the
optimal
treatment
for
patients
end-stage
failure.
However,
limited
availability
of
donor
hearts
presents
major
obstacle
to
meeting
patient
needs.
In
recent
years,
most
groundbreaking
progress
in
has
been
procurement,
significantly
expanding
pool
and
enhancing
clinical
outcomes.
This
review
comprehensively
examines
these
advancements,
including
resurgence
donation
after
circulatory
death
innovative
recovery
evaluation
technologies
such
normothermic
machine
perfusion
thoraco-abdominal
regional
perfusion.
Additionally,
novel
preservation
methods,
controlled
hypothermic
oxygenated
perfusion,
are
evaluated.
The
also
explores
use
extended-criteria
donors,
post-cardiopulmonary
resuscitation
high-risk
all
contributing
increased
without
compromising
Future
directions,
xenotransplantation,
biomarkers,
artificial
intelligence
discussed.
These
innovations
promise
address
current
limitations
optimize
utilization,
ultimately
success.
By
identifying
advancements
proposing
future
research
this
aims
provide
insights
into
advancing
improving
Язык: Английский
Can designer pigs solve the organ shortage?
Lab Animal,
Год журнала:
2024,
Номер
53(10), С. 259 - 262
Опубликована: Сен. 30, 2024
Язык: Английский
Integration of ATAC-Seq and RNA-Seq Reveals VDR–SELENBP1 Axis Promotes Adipogenesis of Porcine Intramuscular Preadipocytes
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12528 - 12528
Опубликована: Ноя. 22, 2024
Intramuscular
fat
(IMF)
content
plays
a
crucial
role
in
determining
pork
quality.
Recent
studies
have
highlighted
transcriptional
mechanisms
controlling
adipogenesis
porcine
IMF.
However,
the
changes
chromatin
accessibility
during
adipogenic
differentiation
are
still
not
well
understood.
In
this
study,
we
performed
assay
for
transposase-accessible
with
high-throughput
sequencing
(ATAC-seq)
and
transcriptome
(RNA-Seq)
analyses
on
intramuscular
preadipocytes
to
explore
their
into
mature
adipocytes.
We
identified
total
of
56,374
differentially
accessible
peaks
4226
expressed
genes
at
day
0
4
differentiation.
A
combined
analysis
ATAC-seq
RNA-seq
data
revealed
that
1750
exhibited
both
differential
RNA
expression
process,
including
selenium-binding
protein
1
(
Язык: Английский