Reliable Polymerase Chain Reaction Methods for Screening for Porcine Endogenous Retroviruses-C (PERV-C) in Pigs

Viruses, Год журнала: 2025, Номер 17(2), С. 164 - 164

Опубликована: Янв. 24, 2025

Porcine endogenous retrovirus C (PERV-C) is a gammaretrovirus present in the genome of many, but not all, pigs. It an ecotropic virus, able to infect only pig cells. In contrast, PERV-A and PERV-B, which are all pigs, can cells multiple host species, including humans, thereby posing risk for xenotransplantation when pigs used as donor animals. Notably, PERV-C recombine with produce PERV-A/C recombinants that human replicate higher titers compared paternal PERV-A. The objective this study evaluate reliability both existing newly developed polymerase chain reactions (PCR) methods detecting PERV-C, aim selecting PERV-C-free be xenotransplantation. To detect by PCR, specific primers targeting region envelope protein gene, differs from PERV-B due its unique receptor binding site, must employed. study, new PCR assays were total ten one real-time assay evaluated their PERV-C. These screen indigenous Greek black Auckland Island German slaughterhouse Two consistently yielded reliable results, whereas other PCRs gave false positive results. Using assays, it was shown out four (using same method previous publication 11 21 found PERV-C-negative), kidney cell line PK15, PERV-C-negative. will enable screening PERV-C-negative Most importantly, genetically modified Germany use clinical trials

Язык: Английский

Screening for porcine endogenous retrovirus - C (PERV-C) in pigs

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

Porcine endogenous retrovirus C (PERV-C) is a gammaretrovirus present in the genome of many, but not all pigs. PERV-C an ecotropic virus, able to infect only pig cells. In contrast, PERV-A and PERV-B are pigs, they can cells numerous host species including humans, therefore pose risk for xenotransplantation using pigs as donor animals. Intriguingly, recombine with resulting PERV-A/C recombinants also human replicate higher titers compared paternal PERV-A. Therefore, PERV-C-free animals should be used xenotransplantation. When screening wild boars German landrace PERV-C, proviral variants were detected, detect these variants, new PCR methods developed. Furthermore, PERVs known active living demonstrated by recombination different copy numbers various organs parts organ. Copy increased age This data was obtained primers amplifying highly conserved polymerase sequence which identical PERV-A, PERV-C. Here we screened indigenous Greek black Auckland Island slaughterhouse presence PCR-based optimized detection methods. PERV-C-negative represent optimal after genetic modifications.

Язык: Английский