Emerging roles of miRNAs in neuropathic pain: From new findings to novel mechanisms

Frontiers in Molecular Neuroscience, Год журнала: 2023, Номер 16

Опубликована: Фев. 17, 2023

Neuropathic pain, which results from damage to the somatosensory nervous system, is a global clinical condition that affects many people. pain imposes significant economic and public health burdens often difficult manage because underlying mechanisms remain unclear. However, mounting evidence indicates role for neurogenic inflammation neuroinflammation in pattern development. There increasing activation of system contribute neuropathic pain. Altered miRNA expression profiles might be involved pathogenesis both inflammatory by regulating neuroinflammation, nerve regeneration, abnormal ion channel expression. lack knowledge about target genes prevents full understanding biological functions miRNAs. At same time, an extensive study on exosomal miRNA, newly discovered role, has advanced our pathophysiology recent years. This section provides comprehensive overview current research discusses potential miRNAs

Язык: Английский

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miRNA Studies in Glaucoma: A Comprehensive Review of Current Knowledge and Future Perspectives

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(19), С. 14699 - 14699

Опубликована: Сен. 28, 2023

Glaucoma, a neurodegenerative disorder that leads to irreversible blindness, remains challenge because of its complex nature. MicroRNAs (miRNAs) are crucial regulators gene expression and associated with glaucoma other diseases. We aimed review discuss the advantages disadvantages miRNA-focused molecular studies in through discussing their potential as biomarkers for early detection diagnosis; offering insights into pathways mechanisms; utility respect personalized medicine, therapeutic potential, non-invasive monitoring. Limitations, such variability, small sample sizes, specificity, limited accessibility ocular tissues, also addressed, underscoring need robust protocols collaboration. Reproducibility validation establish credibility miRNA research findings, integration bioinformatics tools database creation is valuable component comprehensive approach investigate aberrations patients glaucoma. Overall, has provided significant mechanisms disease, biomarkers, diagnostic tools, targets. However, addressing challenges variability tissue essential, further investigations will contribute deeper understanding functional significance miRNAs

Язык: Английский

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Exosomes: potential targets for the diagnosis and treatment of neuropsychiatric disorders

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 29, 2024

Abstract The field of neuropsychiatry is considered a middle ground between neurological and psychiatric disorders, thereby bridging the conventional boundaries matter mind, consciousness, function. Neuropsychiatry aims to evaluate treat cognitive, behavioral, emotional disorders in individuals with conditions. However, pathophysiology these not yet fully understood, objective biological indicators for conditions are currently lacking. Treatment options also limited due blood–brain barrier, which results poor treatment effects. Additionally, many drugs, particularly antipsychotic have adverse reactions, make them difficult tolerate patients. As result, patients often abandon owing reactions. Since discovery exosomes 1983, they been extensively studied various diseases their potential as nanocellulators information exchange cells. Because can freely travel center periphery, brain-derived reflect state brain, has considerable advantages diagnosis treatment. In addition, administration engineered improve therapeutic efficacy, allow lesion targeting, ensure drug stability, prevent systemic Therefore, this article reviews source function exosomes, relationship pathological mechanism neuropsychiatric application disorders.

Язык: Английский

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Exosomes as nanostructures deliver miR-204 in alleviation of mitochondrial dysfunction in diabetic nephropathy through suppressing methyltransferase-like 7A-mediated CIDEC N6-methyladenosine methylation

Aging, Год журнала: 2024, Номер 16(4), С. 3302 - 3331

Опубликована: Фев. 8, 2024

Objective: The exosomal cargo mainly comprises proteins, lipids, and microRNAs (miRNAs). Among these, miRNAs undertake multiple biological effects of exosomes (Exos). Some stem cell-derived have shown the potential to treat diabetic nephropathy (DN). However, there is little research into therapeutic adipose-derived cell (ADSC)-derived on DN. We aimed explore miR-204-modified ADSC-derived Exos mitigate Methods: were extracted identified from ADSCs. Histopathological injury, oxidative stress (OS), mitochondrial function, viability, apoptosis assessed For mechanism exploration, quantitative real-time polymerase chain reaction (qRT-PCR) western blotting used measure miR-204, methyltransferase (METTL3, METTL14, METTL7A), CIDEC. Also, CIDEC m6A methylation miR-204-METTL7A, METTL7A-CIDEC interactions determined. Results: Initially, OS-induced dysfunction was observed in DN rats. inhibited histopathological apoptosis, OS, similar detected vitro model. Intriguingly, miR-204 released by its upregulation enhanced anti-DN Exos. Mechanically, reduced METTL7A expression methylation, thus suppressing OS dysfunction. Conclusions: rescued inhibiting METTL7A-mediated methylation. This study first revealed significant role DN, paving way for development novel strategies improve clinical outcomes patients.

Язык: Английский

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A dual-signal amplification strategy based on rolling circle amplification and APE1-assisted amplification for highly sensitive and specific miRNA analysis for early diagnosis of alzheimer's disease

Talanta, Год журнала: 2024, Номер 272, С. 125747 - 125747

Опубликована: Фев. 10, 2024

Язык: Английский

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