A Peptide HEPFYGNEGALR from Apostichopus japonicus Alleviates Acute Alcoholic Liver Injury by Enhancing Antioxidant Response in Male C57BL/6J Mice

Molecules, Год журнала: 2022, Номер 27(18), С. 5839 - 5839

Опубликована: Сен. 8, 2022

Liver-related disease caused by alcohol is a frequent disorder of the hepatic tract. Heavy consumption in short period causes oxidative damage to liver. Sea cucumber abundant nutrients and its various extracts have been studied for antioxidant properties. One peptide was isolated identified from Apostichopus japonicus our recent study. We investigated benefits model acute ethanol-induced male C57BL/6J mice. Dietary intake could attenuate hepatomegaly, hepatitis accumulation lipid droplets, increase enzyme activities mice with alcoholic liver injury. The results indicated that 20 mg/kg supplement activate Nrf2/HO-1 pathway block nuclear translocation NF-κB alleviate stress inflammation. In addition, preventive effects supplementation may be related autophagy. This study suggests dietary sea cucumber-derived one potential candidates

Язык: Английский

---

Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis

Cell Death and Disease, Год журнала: 2022, Номер 13(12)

Опубликована: Дек. 20, 2022

Abstract Persistent Nrf2 activation is typically noted in many cancers, including colorectal cancer (CRC), aiding cells overcoming growth stress and promoting progression. Sustained activation, which beneficial for cells, called “Nrf2 addiction”; it closely associated with malignancy poor prognosis patients cancer. However, inhibitors may have adverse effects on normal cells. Here, we found that the selenocompound l- selenocystine (SeC) selectively cytotoxic Nrf2-addicted CRC cell line WiDr but not non–Nrf2-addicted mesenchymal stem (MSCs) human colon Another line, C 2 BBe 1 , harbored lower levels of its downstream proteins were less sensitive to SeC, compared We further demonstrated SeC inhibited autophagy Antioxidant GSH pretreatment partially rescued from SeC-induced cytotoxicity pathway inhibition. By contrast, activated MSCs. Transfecting -targeting siRNA decreased persistent alleviated cytotoxicity. In KEAP1 -knockdown increased sensitivity conclusion, attacks constitutively by reducing protein expression through P62–Nrf2–antioxidant response element axis eventually trigger death.

Язык: Английский

---

An update on the bridging factors connecting autophagy and Nrf2 antioxidant pathway

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Авг. 9, 2023

Macroautophagy/autophagy is a lysosome-dependent catabolic pathway for the degradation of intracellular proteins and organelles. Autophagy dysfunction related to many diseases, including lysosomal storage cancer, neurodegenerative cardiomyopathy, chronic metabolic in which increased reactive oxygen species (ROS) levels are also observed. ROS can randomly oxidize proteins, lipids, DNA, causing oxidative stress damage. Cells have developed various antioxidant pathways reduce excessive maintain redox homeostasis. Treatment targeting only one aspect diseases with autophagy shows very limited effects. Herein, identifying bridging factors that regulate both beneficial dual-target therapies. This review intends provide insights into current identified connect Nrf2 pathway, as well their tight interconnection each other. These could be potential dual-purpose targets treatment implicated stress.

Язык: Английский

---

Bacillus atrophaeus NX-12 Utilizes Exosmotic Glycerol from Fusarium oxysporum f. sp. cucumerinum for Fengycin Production

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(28), С. 10565 - 10574

Опубликована: Июль 6, 2023

Bacillus strains are widely used as biological control agents to protect plants from fungal pathogens. However, whether can exploit pathogens increase its biocontrol efficacy remains largely unexplored. Here, atrophaeus NX-12 showed a high inhibition against Fusarium oxysporum f. sp. cucumerinum (FOC). The primary extracellular antifungal component of B. was identified fengycin by matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis. NX-12-secreted not only inhibited the germination FOC spores but also induced production reactive oxygen species (ROS) in cells, leading oxidative stress and accumulation glycerol. Additionally, increased cell wall hydrolase activity, splitting exosmose accumulated glycerol further promoted fengycin. Our results that addition direct FOC, indirectly strengthen antagonistic pathogen exploiting exosmotic FOC.

Язык: Английский

---

Ferroptosis is involved in Staphylococcus aureus-induced mastitis through autophagy activation by endoplasmic reticulum stress

International Immunopharmacology, Год журнала: 2024, Номер 140, С. 112818 - 112818

Опубликована: Июль 30, 2024

Язык: Английский

---