Biology,
Год журнала:
2023,
Номер
12(9), С. 1219 - 1219
Опубликована: Сен. 8, 2023
We
are
witnessing
the
revival
of
CAM
model,
which
has
already
used
been
in
past
by
several
researchers
studying
angiogenesis
and
anti-cancer
drugs
now
offers
a
refined
model
to
fill,
translational
meaning,
gap
between
vitro
vivo
studies.
It
can
be
for
wide
range
purposes,
from
testing
cytotoxicity,
pharmacokinetics,
tumorigenesis,
invasion
action
mechanisms
molecules
validation
new
materials
tissue
engineering
research.
The
is
easy
use,
with
fast
outcome,
makes
experimental
research
more
sustainable
since
it
allows
us
replace,
reduce,
refine
pre-clinical
experimentation
(“3Rs”
rules).
This
review
aims
highlight
some
unique
potential
that
CAM-assay
presents;
particular,
authors
intend
use
future
verify,
microenvironment
comparable
conditions,
albeit
simplified,
angiogenic
ability
functionalized
3D
constructs
regenerative
medicine
strategies
recovery
skeletal
injuries
critical
size
(CSD)
do
not
repair
spontaneously.
For
this
purpose,
organotypic
cultures
will
planned
on
CAMs
set
up
temporal
sequences,
sort
organ
assessing
CSD
utilized
bioreactor
rather
than
vivo.
Biomedicines,
Год журнала:
2021,
Номер
9(11), С. 1666 - 1666
Опубликована: Ноя. 11, 2021
The
use
of
mesenchymal
stromal
cells
(MSCs)
for
tissue
engineering
hyaline
cartilage
is
a
topical
area
regenerative
medicine
that
has
already
entered
clinical
practice.
key
stage
this
procedure
to
create
conditions
chondrogenic
differentiation
MSCs,
increase
the
synthesis
extracellular
matrix
proteins
by
these
and
activate
their
proliferation.
first
such
works
consisted
in
indirect
modification
cells,
namely,
changing
which
they
are
located,
including
microfracturing
subchondral
bone
3D
biodegradable
scaffolds.
most
effective
methods
modifying
cell
culture
MSCs
protein
physical,
have
been
partially
introduced
into
Genetic
despite
effectiveness,
significant
limitations.
Techniques
not
yet
developed
allow
studying
effectiveness
application
even
limited
groups
patients.
MSC
allows
precise
regulation
proliferation,
combination
with
scaffold,
it
obtaining
hyaline-like
regenerate
damaged
area.
This
review
devoted
consideration
comparison
various
used
modify
tissue.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Июнь 15, 2022
Osteoarthritis
(OA),
a
chronic
debilitating
joint
disease
affecting
hundreds
of
million
people
globally,
is
associated
with
significant
pain
and
socioeconomic
costs.
Current
treatment
modalities
are
palliative
unable
to
stop
the
progressive
degeneration
articular
cartilage
in
OA.
Scientific
attention
has
shifted
from
historical
view
OA
as
wear-and-tear
disorder
its
recognition
whole-joint
disease,
highlighting
contribution
other
knee
tissues
pathogenesis.
Despite
much
progress
field
microfluidic
systems/organs-on-a-chip
research
fields,
current
vitro
models
use
do
not
yet
accurately
reflect
complexity
pathophenotype.
In
this
review,
we
provide:
1)
detailed
overview
most
recent
developments
microsystems
approaches
for
modeling,
2)
an
OA-pathophysiology-based
bioengineering
roadmap
requirements
next
generation
more
predictive
authentic
microscale
systems
fit
purpose
only
modeling
but
also
drug
screening
potentially
allow
animal
model
reduction
replacement
near
future.
Biomaterials Advances,
Год журнала:
2022,
Номер
145, С. 213241 - 213241
Опубликована: Дек. 13, 2022
Hydrostatic
pressure
(HP)
and
osmotic
stress
(OS)
play
an
important
role
in
various
biological
processes,
such
as
cell
proliferation
differentiation.
In
contrast
to
canonical
mechanical
signals
transmitted
through
the
anchoring
points
of
cells
with
extracellular
matrix,
physical
molecular
mechanisms
that
transduce
HP
OS
into
cellular
functions
remain
elusive.
Three-dimensional
cultures
show
great
promise
replicate
physiologically
relevant
well-defined
host
bioreactors
goal
shedding
light
on
hidden
aspects
mechanobiology
OS.
This
review
starts
by
introducing
prevalent
for
generation
tissues
are
subject
pathophysiological
loading.
We
then
revisit
mechanotransduction
OS,
describe
current
state
art
biomaterials
control
corresponding
signals.
Biology,
Год журнала:
2023,
Номер
12(9), С. 1219 - 1219
Опубликована: Сен. 8, 2023
We
are
witnessing
the
revival
of
CAM
model,
which
has
already
used
been
in
past
by
several
researchers
studying
angiogenesis
and
anti-cancer
drugs
now
offers
a
refined
model
to
fill,
translational
meaning,
gap
between
vitro
vivo
studies.
It
can
be
for
wide
range
purposes,
from
testing
cytotoxicity,
pharmacokinetics,
tumorigenesis,
invasion
action
mechanisms
molecules
validation
new
materials
tissue
engineering
research.
The
is
easy
use,
with
fast
outcome,
makes
experimental
research
more
sustainable
since
it
allows
us
replace,
reduce,
refine
pre-clinical
experimentation
(“3Rs”
rules).
This
review
aims
highlight
some
unique
potential
that
CAM-assay
presents;
particular,
authors
intend
use
future
verify,
microenvironment
comparable
conditions,
albeit
simplified,
angiogenic
ability
functionalized
3D
constructs
regenerative
medicine
strategies
recovery
skeletal
injuries
critical
size
(CSD)
do
not
repair
spontaneously.
For
this
purpose,
organotypic
cultures
will
planned
on
CAMs
set
up
temporal
sequences,
sort
organ
assessing
CSD
utilized
bioreactor
rather
than
vivo.
