Elsevier eBooks, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1208 - 1208
Опубликована: Янв. 19, 2024
The lacrimal gland is responsible for maintaining the health of ocular surface through production tears. However, our understanding immune system within currently limited. Therefore, in this study, we utilized single-cell RNA sequencing and bioinformatic analysis to identify analyze cells molecules present glands normal mice. A total 34,891 were obtained from mice classified into 18 distinct cell clusters using Seurat clustering. Within these populations, 26 different subpopulations identified, including T cells, innate lymphocytes, macrophages, mast dendritic B cells. Network revealed complex cell-cell interactions between with particularly significant observed among plasma Interestingly, found be main source ligands Thy1 signaling pathway, while M2 macrophages identified as primary target pathway. Moreover, some validated immunohistological techniques. Collectively, findings highlight abundance provide valuable insights complexity its relevance associated diseases.
Язык: Английский
Процитировано
6
Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(2), С. 31 - 31
Опубликована: Фев. 11, 2025
Purpose: Healthy corneas resist colonization by virtually all microbes, yet contact lens wear can predispose the cornea to sight-threatening infection with Pseudomonas aeruginosa. Here, we explored how changes corneal epithelium transcriptional responses P. aeruginosa and its impact on bacterial gene expression. Methods: Male female C57BL/6J mice were fitted a one eye for 24 hours. After removal, immediately challenged 4 hours A separate group of naïve was similarly bacteria. Bacteria-challenged eyes compared uninoculated controls, as alone. Total RNA sequencing determined Results: Prior profoundly altered response aeruginosa, including upregulated pattern recognition receptors (tlr3, nod1); downregulated lectin pathway complement activation (masp1); amplified upregulation tcf7, gpr55, ifi205, wfdc2 (immune defense); further suppression efemp1 (corneal stromal integrity). Without wear, mitochondrial ubiquinone metabolism genes. Lens alone axl, grn, gpr55 defense) Ca2+-dependent genes necab1, snx31, npr3. exposure prior wearing versus virulence (popD), regulation (rsmY, PA1226), antimicrobial resistance (arnB, oprR). Conclusions: impacts expression, altering responds it favoring virulence, survival, adaptation. Impacted associated networks provide avenues research better understand pathogenesis.
Язык: Английский
Процитировано
0
Experimental Eye Research, Год журнала: 2024, Номер 248, С. 110073 - 110073
Опубликована: Сен. 6, 2024
Язык: Английский
Процитировано
2
Cells, Год журнала: 2024, Номер 13(9), С. 748 - 748
Опубликована: Апрель 25, 2024
Stem cells (SCs) undergo asymmetric division, producing transit-amplifying (TACs) with increased proliferative potential that move into tissues and ultimately differentiate a specialized cell type. Thus, TACs represent an intermediary state between stem differentiated cells. In the cornea, population of resides in limbal region, named epithelial (LESCs). As LESCs proliferate, they generate centripetally cornea corneal Upon injury, research suggests progenitor-like exists within can centrifugally limbus, where dedifferentiate LESCs. Herein, we summarize recent advances made understanding mechanism governs differentiation TACs, thereafter, We also outline evidence support existence whether could capabilities cornea. Furthermore, to gain further insights dynamics most findings other organ systems hypothesis SCs.
Язык: Английский
Процитировано
1
Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(11), С. 43 - 43
Опубликована: Сен. 27, 2024
Corneal wounding healing is critical for maintaining clear vision, however, a complete understanding of its dynamic regulatory mechanisms remains elusive. Here, we used single-cell RNA sequencing (scRNA-seq) to analyze the cellular activities and transcriptional changes corneal limbal epithelial cells at different stages after wound in cynomolgus monkeys, which exhibit closer transcriptomic similarity humans.
Язык: Английский
Процитировано
1
American Journal Of Pathology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
1
Biology, Год журнала: 2024, Номер 13(3), С. 145 - 145
Опубликована: Фев. 26, 2024
The corneal epithelium (CE) is spread between two domains, the outer vascularized limbus and avascular cornea proper. Epithelial cells undergo constant migration from to vision-critical central cornea. Coordinated with this migration, differentiation changes where a pool of unique stem/precursor at yields mature that reach center. Differentiation heralded by expression corneal-specific Krt12. Processing data acquired scRNA-Seq showed increase in Krt12 occurs four distinct steps within limbus, plus single continuous Differential gene analysis demonstrated these domains reflect discreet stages CE yielded extensive information genes undergoing down- or upregulation sequential transition less more differentiate conditions. approach allowed identification multiple cohorts, including (a) which have maximal most primitive, Krt12-negative cell cohort, likely include cells; (b) sets decrease along whole path; (c) showing positive negative correlation
Язык: Английский
Процитировано
0
Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(12), С. 29 - 29
Опубликована: Окт. 21, 2024
This study aimed to define the heterogeneity, spatial localization, and functional roles of immune cells in mouse cornea using single-cell RNA sequencing (scRNA-seq) immunofluorescent staining.
Язык: Английский
Процитировано
0
Journal of Ophthalmology, Год журнала: 2024, Номер 2024(1)
Опубликована: Янв. 1, 2024
The maintenance of corneal transparency and normal vision is dependent on preservation epithelial endothelial cell layer homeostases. Different types injury can induce swelling losses in transparency. Fuchs dystrophy (FECD) one type that commonly treated with rho‐associated coiled–coil‐containing protein kinase (ROCK) inhibitors. While their clinical benefit apparent, certain aspects mechanism action require clarification. Specifically, although topical eye drops containing ROCK inhibitors have been employed to treat dysfunction–associated edema, it remains unclear whether interactions between both contribute mitigating signs compromise vision. To address this question, we first review the intricate signaling pathways role modulating a variety functions are related We also results ongoing trials employing current FDA‐approved inhibitors, highlighting prominent Y‐27632 treatment ocular conditions, particularly FECD, its promising reversing through facilitating proliferation suppressing apoptosis. This shows inhibitor affected by epithelium endothelium. realization makes likely will be approved for use setting FECD.
Язык: Английский
Процитировано
0
STAR Protocols, Год журнала: 2024, Номер 5(4), С. 103444 - 103444
Опубликована: Ноя. 7, 2024
Язык: Английский
Процитировано
0