Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients
Cancer Cell,
Год журнала:
2024,
Номер
42(3), С. 444 - 463.e10
Опубликована: Фев. 29, 2024
Язык: Английский
Cell cross talk within the lymphoma tumor microenvironment: follicular lymphoma as a paradigm
Blood,
Год журнала:
2023,
Номер
143(12), С. 1080 - 1090
Опубликована: Дек. 14, 2023
Язык: Английский
The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques
PLoS Biology,
Год журнала:
2025,
Номер
23(3), С. e3003070 - e3003070
Опубликована: Март 19, 2025
Multiplexed
imaging
is
a
powerful
approach
in
spatial
biology,
although
it
complex,
expensive
and
labor-intensive.
Here,
we
present
the
IBEX
Knowledge-Base,
central
resource
for
reagents,
protocols
more,
to
enhance
knowledge
sharing,
optimization
innovation
of
proteomics
techniques.
Язык: Английский
Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients
Annals of Medicine,
Год журнала:
2025,
Номер
57(1)
Опубликована: Апрель 15, 2025
Non-Hodgkin
lymphomas
(NHLs)
are
the
most
common
hematological
malignancies
worldwide.
Among
these,
B-cell
(B-NHLs)
second
leading
cause
of
death
in
hematologic
neoplasms.
In
this
study,
a
detailed
immunophenotypic
analysis
lymphocytes
bone
marrow
aspirate
(BMA)
75
patients
with
four
different
subtypes
B-NHLs
was
performed
at
diagnosis.
The
samples
were
analyzed
by
flow
cytometry
(FC)
using
stain-lyse-no
wash
technique
and
comprehensive
six-color
antibody
panel.
Our
data
showed
trend
percentage
values
distinct
lymphocyte
subsets,
which
did
not
seem
to
correlate
worse
prognosis,
except
for
B
cells
diffuse
large
lymphoma
(DLBCL),
significantly
higher
stage
IV
than
stages
II
III.
ROC
curve
that
value
could
be
used
predict
disease.
Total
greater
presented
lower
disease
progression,
specifically
mantle
cell
(MCL)
marginal
zone
(MZL).
contrast,
natural
killer
(NK)
T
DLBCL
follicular
(FL),
progressed
more
frequently.
Interestingly,
double
positive
(DPT)
helper
(Th),
we
observed
good
prognosis.
Specifically,
univariate
Cox
regression
analyses
indicated
Th
diagnosis
better
prognostic
predictor
DLBCL.
These
preliminary
findings
encourage
us
further
investigate
role
subpopulations
NHL.
Язык: Английский
The essential microenvironmental role of oligomannoses specifically inserted into the antigen-binding sites of lymphoma cells
Blood,
Год журнала:
2023,
Номер
143(12), С. 1091 - 1100
Опубликована: Ноя. 22, 2023
Язык: Английский
Impact of positive CD4 cells on event‐free survival in follicular lymphoma patients
Cancer Medicine,
Год журнала:
2024,
Номер
13(17)
Опубликована: Сен. 1, 2024
Abstract
Objective
Previous
results
about
prognostic
value
of
CD4+
T
cells
in
follicular
lymphoma
(FL)
remain
controversial.
Methods
Immunohistochemistry
was
used
to
examine
expression
positive
CD4
103
patients
with
FL
1‐3A.
Early
failure
described
as
failing
achieve
event‐free
survival
(EFS)
at
12
or
24
months.
Results
There
were
49
(47.6%)
male
and
54
(52.4%)
females,
a
median
age
years.
Compared
<20%
cells,
≥20%
exhibited
significant
lower
risk
early
(2‐year
EFS
rate:
56.7%
vs
73.5%,
p
=
0.047).
When
stratified
based
on
cell
combined
FLIPI,
the
(
0.002)
OS
0.007)
significantly
different.
Conclusions
This
study
demonstrated
that
higher
predicts
lymphoma,
combination
analysis
FLIPI
could
better
predict
disease
relapse
outcome.
Язык: Английский
AI-Based Computational H&E Staining Enables Spatial Transcriptomic Analysis in Classic Hodgkin Lymphoma
International Journal of Surgical Pathology,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 21, 2024
Язык: Английский
The follicular lymphoma and chronic lymphocytic leukemia proliferative microenvironment at single-cell resolution
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 17, 2024
Abstract
Lymph
nodes
(LNs)
are
secondary
lymphoid
organs
where
lymphocytes
interact
with
antigen
presenting
cells
to
initiate
adaptive
immune
responses
within
microenvironments
established
by
resident
stromal
cells.
LNs
also
the
major
site
of
growth
follicular
lymphoma
(FL)
and
chronic
lymphocytic
leukemia
(CLL),
B
cell
neoplasms
that
alter
architecture
in
highly
stereotypic
ways.
To
characterize
FL
CLL
LN
microenvironment,
we
developed
a
pipeline
for
single-cell
RNA
sequencing
all
We
observed
proliferation
specialized
niches
commences
transient
upregulation
MYC,
subsequent
downregulation
which
may
act
limit
potential
these
indolent
neoplasms.
Proliferating
neoplastic
follicles
co-localized
dendritic
cells,
whereas
proliferating
were
spatially
associated
distinct
set
fibroblasts
expressing
CCL19
localized
centers.
used
informatic
analyses
microscopy
identify
validate
interacting
sets
ligand-receptor
pairs
between
fibroblasts,
including
interactions
involving
CD74-MIF,
TNFRSF13C
(BAFF
receptor),
immunomodulatory
factors
such
as
CD55
Galectin-9
(Gal9),
adhesion
molecules.
Our
highlight
common
features
two
microenvironment-dependent
provide
roadmap
identifying
vulnerabilities
new
therapeutic
strategies.
Key
points
Nodal
have
similar
proliferative
programs
predicted
Proliferation
occurs
lymph
node
defined
different
types
fibroblasts.
Язык: Английский
Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single Living Cells
ACS Sensors,
Год журнала:
2024,
Номер
9(12), С. 6759 - 6767
Опубликована: Дек. 9, 2024
Detections
of
internal
substances
and
morphologies
for
label-free
living
cells
are
crucial
revealing
malignant
diseases.
With
the
phase
serving
as
a
coupling
refractive
index
(RI)
(marker
substances)
thickness
(morphology),
existing
decoupling
methods
mainly
rely
on
complex
integrated
systems
or
extensive
optical
field
information.
Developing
simple
rapid
remains
challenge.
This
study
introduces
morphology
imaging
microscope
(RIMIM)
system
utilizing
polarization
multiplexing
single
cells.
By
simultaneous
degree
circular
(DOCP)
noninterferometric
quantitative
(QPI),
intracellular
distribution
(IRID)
can
be
decoupled.
The
calculated
from
is
input
into
depolarization
decay
model
(CDDM)
to
retrieve
IRID.
Subsequently,
decoupled
result
using
retrieved
Experiments
conducted
mouse
forestomach
carcinoma
(MFC)
human
kidney-2
(HK-2)
demonstrated
RIMIM
system's
ability
IRID
decouple
fine
morphology.
Additionally,
effectively
detected
membrane
damage
changes
in
erastin-induced
ferroptotic
HK-2
cells,
with
average
root-mean-square
surface
folds
65.5%
70.0%
higher
than
those
normal
Overall,
provides
method
RI
morphology,
showing
great
potential
live
cells'
cytopathology
detection.
Язык: Английский