Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single Living Cells DOI
Huijun Wang, Lu Zhang, Chen Fan

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(12), P. 6759 - 6767

Published: Dec. 9, 2024

Detections of internal substances and morphologies for label-free living cells are crucial revealing malignant diseases. With the phase serving as a coupling refractive index (RI) (marker substances) thickness (morphology), existing decoupling methods mainly rely on complex integrated systems or extensive optical field information. Developing simple rapid remains challenge. This study introduces morphology imaging microscope (RIMIM) system utilizing polarization multiplexing single cells. By simultaneous degree circular (DOCP) noninterferometric quantitative (QPI), intracellular distribution (IRID) can be decoupled. The calculated from is input into depolarization decay model (CDDM) to retrieve IRID. Subsequently, decoupled result using retrieved Experiments conducted mouse forestomach carcinoma (MFC) human kidney-2 (HK-2) demonstrated RIMIM system's ability IRID decouple fine morphology. Additionally, effectively detected membrane damage changes in erastin-induced ferroptotic HK-2 cells, with average root-mean-square surface folds 65.5% 70.0% higher than those normal Overall, provides method RI morphology, showing great potential live cells' cytopathology detection.

Language: Английский

Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients DOI
Andrea J. Radtke, Ekaterina Postovalova, Arina Varlamova

et al.

Cancer Cell, Journal Year: 2024, Volume and Issue: 42(3), P. 444 - 463.e10

Published: Feb. 29, 2024

Language: Английский

Citations

21
Cell cross talk within the lymphoma tumor microenvironment: follicular lymphoma as a paradigm DOI
Camille Laurent, Sascha Dietrich, Karin Tarte

et al.

Blood, Journal Year: 2023, Volume and Issue: 143(12), P. 1080 - 1090

Published: Dec. 14, 2023

Language: Английский

Citations

16
The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques DOI Creative Commons
Andrea J. Radtke, Ifeanyichukwu U. Anidi, Leanne Arakkal

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(3), P. e3003070 - e3003070

Published: March 19, 2025

Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.

Language: Английский

Citations

0
Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients DOI Creative Commons
Luciana Valvano, Rocchina Vilella, Fiorella D’Auria

et al.

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: April 15, 2025

Non-Hodgkin lymphomas (NHLs) are the most common hematological malignancies worldwide. Among these, B-cell (B-NHLs) second leading cause of death in hematologic neoplasms. In this study, a detailed immunophenotypic analysis lymphocytes bone marrow aspirate (BMA) 75 patients with four different subtypes B-NHLs was performed at diagnosis. The samples were analyzed by flow cytometry (FC) using stain-lyse-no wash technique and comprehensive six-color antibody panel. Our data showed trend percentage values distinct lymphocyte subsets, which did not seem to correlate worse prognosis, except for B cells diffuse large lymphoma (DLBCL), significantly higher stage IV than stages II III. ROC curve that value could be used predict disease. Total greater presented lower disease progression, specifically mantle cell (MCL) marginal zone (MZL). contrast, natural killer (NK) T DLBCL follicular (FL), progressed more frequently. Interestingly, double positive (DPT) helper (Th), we observed good prognosis. Specifically, univariate Cox regression analyses indicated Th diagnosis better prognostic predictor DLBCL. These preliminary findings encourage us further investigate role subpopulations NHL.

Language: Английский

Citations

0
The essential microenvironmental role of oligomannoses specifically inserted into the antigen-binding sites of lymphoma cells DOI
Freda K. Stevenson, Francesco Forconi

Blood, Journal Year: 2023, Volume and Issue: 143(12), P. 1091 - 1100

Published: Nov. 22, 2023

Language: Английский

Citations

5
Impact of positive CD4 cells on event‐free survival in follicular lymphoma patients DOI Creative Commons
Cong Li, Na Guo,

Shuiyun Han

et al.

Cancer Medicine, Journal Year: 2024, Volume and Issue: 13(17)

Published: Sept. 1, 2024

Abstract Objective Previous results about prognostic value of CD4+ T cells in follicular lymphoma (FL) remain controversial. Methods Immunohistochemistry was used to examine expression positive CD4 103 patients with FL 1‐3A. Early failure described as failing achieve event‐free survival (EFS) at 12 or 24 months. Results There were 49 (47.6%) male and 54 (52.4%) females, a median age years. Compared <20% cells, ≥20% exhibited significant lower risk early (2‐year EFS rate: 56.7% vs 73.5%, p = 0.047). When stratified based on cell combined FLIPI, the ( 0.002) OS 0.007) significantly different. Conclusions This study demonstrated that higher predicts lymphoma, combination analysis FLIPI could better predict disease relapse outcome.

Language: Английский

Citations

1
AI-Based Computational H&E Staining Enables Spatial Transcriptomic Analysis in Classic Hodgkin Lymphoma DOI
Michael E. Kallen, Laura Wake, Rima Koka

et al.

International Journal of Surgical Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Language: Английский

Citations

0
The follicular lymphoma and chronic lymphocytic leukemia proliferative microenvironment at single-cell resolution DOI Creative Commons
Antonio Ferreira, Shumei Wang, Larysa Poluben

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract Lymph nodes (LNs) are secondary lymphoid organs where lymphocytes interact with antigen presenting cells to initiate adaptive immune responses within microenvironments established by resident stromal cells. LNs also the major site of growth follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL), B cell neoplasms that alter architecture in highly stereotypic ways. To characterize FL CLL LN microenvironment, we developed a pipeline for single-cell RNA sequencing all We observed proliferation specialized niches commences transient upregulation MYC, subsequent downregulation which may act limit potential these indolent neoplasms. Proliferating neoplastic follicles co-localized dendritic cells, whereas proliferating were spatially associated distinct set fibroblasts expressing CCL19 localized centers. used informatic analyses microscopy identify validate interacting sets ligand-receptor pairs between fibroblasts, including interactions involving CD74-MIF, TNFRSF13C (BAFF receptor), immunomodulatory factors such as CD55 Galectin-9 (Gal9), adhesion molecules. Our highlight common features two microenvironment-dependent provide roadmap identifying vulnerabilities new therapeutic strategies. Key points Nodal have similar proliferative programs predicted Proliferation occurs lymph node defined different types fibroblasts.

Language: Английский

Citations

0
Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single Living Cells DOI
Huijun Wang, Lu Zhang, Chen Fan

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(12), P. 6759 - 6767

Published: Dec. 9, 2024

Detections of internal substances and morphologies for label-free living cells are crucial revealing malignant diseases. With the phase serving as a coupling refractive index (RI) (marker substances) thickness (morphology), existing decoupling methods mainly rely on complex integrated systems or extensive optical field information. Developing simple rapid remains challenge. This study introduces morphology imaging microscope (RIMIM) system utilizing polarization multiplexing single cells. By simultaneous degree circular (DOCP) noninterferometric quantitative (QPI), intracellular distribution (IRID) can be decoupled. The calculated from is input into depolarization decay model (CDDM) to retrieve IRID. Subsequently, decoupled result using retrieved Experiments conducted mouse forestomach carcinoma (MFC) human kidney-2 (HK-2) demonstrated RIMIM system's ability IRID decouple fine morphology. Additionally, effectively detected membrane damage changes in erastin-induced ferroptotic HK-2 cells, with average root-mean-square surface folds 65.5% 70.0% higher than those normal Overall, provides method RI morphology, showing great potential live cells' cytopathology detection.

Language: Английский

Citations

0