Fibre-based fluorescence-lifetime imaging microscopy: a real-time biopsy guidance tool for suspected lung cancer

Translational Lung Cancer Research, Год журнала: 2024, Номер 13(2), С. 355 - 361

Опубликована: Фев. 1, 2024

: Lung cancer is the most common cause of cancer-related deaths worldwide. Early detection improves outcomes, however, existing sampling techniques are associated with suboptimal diagnostic yield and procedure-related complications. Autofluorescence-based fluorescence-lifetime imaging microscopy (FLIM), a technique which measures endogenous fluorophore decay rates, may aid identification optimal biopsy sites in suspected lung cancer. Our fibre-based system, utilising 488 nm excitation, deliverable via platforms, enables real-time visualisation lifetime analysis distal alveolar structure. We evaluated accuracy system to detect changes fluorescence freshly resected ex vivo adjacent healthy tissue as first step towards future translation. The study compares paired non-small cell (NSCLC) non-cancerous tissues gold standard pathology assess performance technique. Paired NSCLC were obtained from thoracic resection patients (N=21). A clinically compatible endomicroscopy platform was used acquire simultaneous intensity images. Fluorescence lifetimes calculated using computationally-lightweight, rapid determination method. significantly reduced cancer, compared [mean ± deviation (SD), 1.79±0.40 vs. 2.15±0.26 ns, P<0.0001], images demonstrated distortion elastin autofluorescence Fibre-based good characteristics for distinguishing tissue, 81.0% sensitivity 71.4% specificity. novel label-free quantitative analysis, discriminates tissue. This minimally invasive has potential be translated guidance tool, capable optimising

Язык: Английский

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Bronchoscopy with and without needle-based confocal laser endomicroscopy for peripheral lung nodule diagnosis: protocol for a multicentre randomised controlled trial (CLEVER trial)

BMJ Open, Год журнала: 2024, Номер 14(7), С. e081148 - e081148

Опубликована: Июль 1, 2024

Introduction Despite many technological advances, the diagnostic yield of bronchoscopic peripheral lung nodule analysis remains limited due to frequent mispositioning. Needle-based confocal laser endomicroscopy (nCLE) enables real-time microscopic feedback on needle positioning, potentially improving sampling location and yield. Previous studies have defined validated nCLE criteria for malignancy, airway parenchyma. Larger demonstrating effect are lacking. We aim investigate if nCLE-imaging integrated with conventional bronchoscopy results in a higher compared without nCLE. Methods This is parallel-group randomised controlled trial. Recruitment performed at pulmonology outpatient clinics universities general hospitals six different European countries one hospital USA. Consecutive patients malignancy suspected (10–30 mm) an indication will be screened, 208 included. Web-based randomisation (1:1) between two procedures performed. The primary outcome Secondary outcomes include sensitivity repositionings, procedure fluoroscopy duration, complications. Pathologists blinded type; endoscopists not. Ethics dissemination Primary approval by Committee Amsterdam University Medical Center. Dissemination involves publication peer-reviewed journal. Support Financial material support from Mauna Kea Technologies. Trial registration number NCT06079970 .

Язык: Английский

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Confocal Laser Endomicroscopy in Resection of Sinonasal Malignant Melanoma—Preliminary Report on Real-Time Margin Assessment and Support in Surgical Decision-Making

Journal of Clinical Medicine, Год журнала: 2024, Номер 13(15), С. 4483 - 4483

Опубликована: Июль 31, 2024

Background/Objectives: Building upon the rising value of Confocal Laser Endomicroscopy (CLE) in squamous cell carcinoma head and neck, we present first application CLE during resection sinonasal malignant melanomas. This study aims to evaluate potential assist surgeons intraoperative decision-making, with a particular focus on margin assessment within constrained nasal cavity. Methods: Two cases melanoma were included this study. was employed examine visible tumors their margins, both pre- post-endoscopic resection. The findings compared histopathological results as well data carcinoma, for which malignancy criteria had already been established prior projects. Results: provided real-time visualization melanomas successfully differentiating between healthy neoplastic tissue findings. Conclusion: offers assessment, aiding more precise tumor potentially improving patient outcomes. demonstrates feasibility using melanoma, highlighting its ability differentiate intraoperatively.

Язык: Английский

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A preclinical animal study to evaluate the operability and safety of domestic one-way endobronchial valves

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Май 1, 2024

To evaluate the operability and safety of bronchoscopic domestic one-way endobronchial valves (EBV) on animals.

Язык: Английский

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A case of congenital bronchial atresia with tracheobronchial stenosis caused by emphysema: Successful management with thoracoscopic surgery

Heliyon, Год журнала: 2024, Номер 10(11), С. e32753 - e32753

Опубликована: Июнь 1, 2024

Язык: Английский

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Méthodes innovantes de diagnostic et de phénotypage du rejet pulmonaire : au-delà des biopsies transbronchiques

Revue des Maladies Respiratoires Actualités, Год журнала: 2024, Номер 16(1), С. 1S90 - 1S94

Опубликована: Сен. 1, 2024

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Pulmonary endoscopy – central to an interventional pulmonology program

Expert Review of Respiratory Medicine, Год журнала: 2024, Номер 18(11), С. 843 - 860

Опубликована: Окт. 7, 2024

Introduction Pulmonary endoscopy occupies a central role in Interventional Pulmonology and is frequently the mainstay of diagnosis respiratory disease, particular lung malignancy. Older techniques such as rigid bronchoscopy maintain an important airway obstruction. Renewed interest peripheral pulmonary nodule driving major advances technologies to increase diagnostic accuracy advance new potential endoscopic therapeutic options.

Язык: Английский

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Technological advancements in endoscopy and their impact on gastrointestinal nursing

Gastrointestinal Nursing, Год журнала: 2024, Номер 22(Sup8), С. S29 - S34

Опубликована: Окт. 1, 2024

Technological advancements in endoscopy have significantly transformed the landscape of gastrointestinal (GI) nursing. This article explores latest innovations endoscopic techniques, such as high-resolution imaging, ultrasound and therapeutic procedures. It examines implications these for GI nursing practice, focusing on roles responsibilities nurses pre-procedural, intra-procedural post-procedural care. The importance continuous education specialised training is also discussed. Recommendations are provided to enhance practices settings, ensuring optimal patient care safety.

Язык: Английский

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Diagnostic modalities in the mediastinum and the role of bronchoscopy in mediastinal assessment: a narrative review

Mediastinum, Год журнала: 2024, Номер 8, С. 51 - 51

Опубликована: Дек. 1, 2024

Diagnosis of pathology in the mediastinum has proven quite challenging, given wide variability both benign and malignant diseases that affect a diverse array structures. This complexity led to development many different non-invasive invasive diagnostic modalities. Historically, diagnosis relied on imaging modalities such as chest X-ray, computed tomography (CT), magnetic resonance imaging, positron emission topography. Once suspicious lesion was identified with one these techniques, gold standard for mediastinoscopy staging disease. More recently, minimally techniques CT-guided biopsy, endobronchial ultrasound transbronchial needle aspiration, endoscopic fine aspiration have revolutionized mediastinum. review provides comprehensive analysis all available diagnosing mediastinal disease an emphasis bronchoscopic techniques. Literature search performed via PubMed database. We included types articles study designs, including original research, meta-analyses, reviews, abstracts. Minimally ultrasound-transbronchial (EBUS-TBNA) ultrasound-fine (EUS-FNA) demonstrated high yield low complication rate made significant difference time lives patients. There continues be innovation field bronchoscopy new technologies confocal laser endomicroscopy, optical coherence tomography, artificial intelligence. Bronchoscopy is will continue integral modality

Язык: Английский

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Comparison of Synapse 3D system (Version 4.4) and DirectPath system (Version 2.0) in virtual bronchoscopic navigation application for peripheral pulmonary nodules

Therapeutic Advances in Respiratory Disease, Год журнала: 2024, Номер 18

Опубликована: Янв. 1, 2024

Background: Differences between virtual bronchoscopic navigation (VBN) systems and their impacts on the diagnostic yield of transbronchial biopsy (TBB) peripheral pulmonary nodules (PPNs) remain unclear. Objectives: To compare Synapse 3D system (Version 4.4, Fujifilm, Japan) DirectPath 2.0, Olympus, in VBN application PPNs. Design: Retrospective study with self-paired design exploratory retrospective cohort design. Methods: The analyzed patients PPNs using (Group S) D) compared differences two groups bronchial tree reconstruction, pathway planning, VBN-assisted TBB Results: In all, 289 were ultimately. Bronchial reconstruction quality was better Group S ( p < 0.001). Navigation planning duration longer than that D (median 1.35 vs 1.04 s, Automated success rate higher (36.7% 19.7%, 0.001), CT image parameter nodule diameter, bronchus sign, distance from hilum had significant effects it both groups. Fifty-six forty-two localization not significantly different (85.3% 91.2% 67.6% 61.8%, respectively, > 0.05). Conclusion: 4.4) 2.0) own merits. Localization no statistical difference for these systems. Improvements segmentation algorithms most suitable chest scan data them may be breakthrough to improve efficiency VBN, especially poor experienced interventional physicians.

Язык: Английский

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