Methylene blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache
The Analyst,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Interleukin-6
(IL-6)
protects
neurons
by
inhibiting
the
expression
of
factors
related
to
neuronal
injury
in
nervous
headache
patients.
The
development
rapid
and
sensitive
IL-6
detection
methods
will
be
very
advantageous
for
easing
pain
such
In
this
work,
an
immobilization-free
immunodetection
method
is
explored
voltammetric
screening
serum
samples
from
Initially,
methylene-blue-encapsulated
liposomes
(MBLS)
labeled
with
anti-IL-6
antibodies
are
confined
capture
antibody-coated
microplate
through
a
sandwich-type
immunoreaction,
subsequently
subjected
lysis
treatment.
After
that,
lytic
solution
transferred
into
cell
including
Nafion-modified
working
electrode.
Methylene
blue
molecules
positive
charge
captured
on
negatively
charged
Nafion
membrane,
thus
generating
signal.
peak
currents
relative
amount
solution.
Under
optimized
experimental
situations,
MBLS-based
split-type
electrochemical
sensing
protocols
have
acceptable
0.01
100
pg
mL-1,
allow
at
concentration
as
low
9.1
fg
mL-1.
batch-to-batch
coefficients
variation
were
≤11.95%.
Good
anti-interference
capability
was
achieved
against
other
biomolecules.
Seven
human
specimens
two
diluted
obtained
patients
determined
immunoassay,
well-matched
results
comparison
those
ELISA
protocol.
Язык: Английский
Fraxin Alleviates Atherosclerosis by Inhibiting Oxidative Stress and Inflammatory Responses via the TLR4/PI3K/Akt Pathway
Current Issues in Molecular Biology,
Год журнала:
2025,
Номер
47(5), С. 308 - 308
Опубликована: Апрель 27, 2025
Fraxin
is
a
bioactive
compound
derived
from
Cortex
Fraxini.
It
known
for
its
diverse
biological
activities
and
numerous
benefits,
including
anti-inflammatory,
antioxidant,
analgesic,
antimicrobial,
antiviral,
immunomodulatory
effects.
Despite
growing
interest
in
natural
compounds
cardiovascular
diseases
Fraxin’s
atheroprotective
properties
molecular
targets
have
not
yet
been
fully
elucidated.
To
address
this
gap,
our
research
employed
an
integrated
approach
combining
network
pharmacology,
docking
simulations,
vitro
validation
to
systematically
unravel
therapeutic
mechanisms
against
atherosclerosis
(AS).
The
results
showed
that
84
potential
AS
were
predicted
through
public
databases,
the
key
target
TLR4
was
identified
by
protein–protein
interaction
analysis.
GO
enrichment
KEGG
pathway
analysis
revealed
these
significantly
enriched
PI3K-Akt
oxidative
stress
responses
pathways.
Subsequently
conducted
studies
validated
modulates
TLR4/PI3K/Akt
signaling
suppress
reactive
oxygen
species
generation
downregulate
pro-inflammatory
cytokines
Il1b,
Il6,
Tnf
thereby
slowing
atherosclerotic
disease
advancement.
This
investigation
methodically
delineates
underlying
management,
establishing
scientific
foundation
translation
into
clinical
practice.
Язык: Английский
Tumor microenvironment and immune-related myositis: addressing muscle wasting in cancer immunotherapy
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Май 2, 2025
Cancer
immunotherapy,
which
leverages
the
immune
system
to
target
neoplastic
cells,
has
undergone
significant
transformation
in
recent.
However,
immunotherapy
may
have
negative
effects
on
skeletal
muscle
function,
causing
wasting
and
functional
decline
cancer
patients.
In
this
study,
we
review
mechanisms
by
influences
muscle,
focusing
immune-related
myositis,
inflammation,
metabolic
alterations
within
tumor
microenvironment
(TME).
The
key
methodologies,
including
biomechanical
assessment
techniques
such
as
electrical
impedance
myography
ultrasound
imaging,
are
discussed
provide
valuable
insights
into
process
that
maintain
integrity
function
patients
receiving
immunotherapy.
Moreover,
dual
of
suppression
damage
described,
revealing
significance
inflammatory
cytokines,
checkpoints,
disturbances
TME.
Importantly,
propose
combination
therapies
integrating
nutritional
interventions
or
anti-inflammatory
potential
approaches
for
mitigating
wasting.
This
study
highlights
need
deeper
investigations
optimize
improve
its
efficacy
preserving
health,
thereby
improving
patient
outcomes
quality
life.
Язык: Английский
Antimicrobial Peptides Expressed by the Polyaminoglycoside Nanosystem for Bacterial Peritonitis Management via Inflammation Modulation
Molecular Pharmaceutics,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 30, 2025
Peritonitis,
a
prevalent
and
dangerous
condition,
can
result
from
trauma,
surgery,
liver
cirrhosis,
peritoneal
dialysis,
gastrointestinal
issues.
If
not
effectively
managed,
it
cause
systemic
infection,
leading
to
complications,
such
as
sepsis
bacteremia.
LL37,
an
antimicrobial
peptide
of
the
innate
immune
system,
exhibits
broad-spectrum
antibacterial
activity
potent
immune-modulating
functions,
serving
critical
defense
against
infections.
In
this
study,
we
developed
combined
system
delivering
therapeutic
plasmid
(pLL37)
via
cationic
poly(aminoglycoside)
(SS-HPT)
treat
bacterial
peritonitis
in
immunocompromised
populations.
This
combines
direct
action
inflammation
modulation.
The
SS-HPT
carrier,
containing
tobramycin,
provides
immediate
effects
by
inhibiting
protein
synthesis.
Simultaneously,
expressed
LL37
enhances
membrane
disruption
modulates
inflammatory
response
regulating
cytokine
release
cell
differentiation.
Treatment
efficacy
was
comprehensively
evaluated
using
survival
rate
assessments,
clinical
symptom
analysis,
marker
detection,
histopathological
observation,
molecular
biological
methods.
Results
indicated
that
significantly
improved
rates,
reduced
responses,
ameliorated
damage,
preventing
treating
its
complications.
conclusion,
SS-HPT/pLL37
offers
promising
new
strategy
for
treatment
peritonitis.
Язык: Английский