Dual-Lock System for High Sensitivity and Selectivity in Redox Enzyme Activation and Imaging

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Reductase expression is a potential indicator of cellular pathology. Single-detection systems for reductases have been developed, however, the development dual-detection remain largely unexplored. We rationally designed dual-lock fluorescent probe that exhibited high signal-to-noise ratio with fluorescence Off-On response exclusively simultaneous activity two reductases, NTR and hNQO1, which are overexpressed in cancer hypoxia. The system comprised naphthalimide fluorophore control mediated by PET ICT, trimethyl-locked quinone group sensitive to nitrobenzyl carbamate NTR. This study employed hypoxia model HeLa cells demonstrate our developed detected more effectively than single-detection systems. Moreover, it enabled noninvasive real-time monitoring zebrafish embryos. Consequently, probe, strategically provides only when both NQO1 active, offers novel diagnostic platform vitro vivo applications, detecting various pathological states.

Язык: Английский

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Green Engineered CuO/SnO₂/HNT Composites: Illuminating the Photocatalytic Path for Organic Pollutant Remediation and Zebrafish Embryo Toxicity Evaluation

Langmuir, Год журнала: 2024, Номер 40(44), С. 23337 - 23355

Опубликована: Окт. 24, 2024

Photocatalysis stands out as a promising technique for treating organic contaminants, yet the quest visible light active composite materials, crafted through cost-effective, eco-friendly, and uncomplicated processes, poses formidable challenges. Here, we introduce successful endeavor, synthesis of CuO/SnO

Язык: Английский

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Feasibility study on integrating morphological and transcriptomic data for identifying teratogenic molecular markers in zebrafish embryo toxicity testing

Computational Toxicology, Год журнала: 2025, Номер unknown, С. 100342 - 100342

Опубликована: Март 1, 2025

Язык: Английский

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The fate and ecological risk of mefentrifluconazole in the water-sediment system: A systematic analysis at the enantiomer level

Environmental Research, Год журнала: 2025, Номер unknown, С. 121682 - 121682

Опубликована: Апрель 1, 2025

Язык: Английский

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