Genetic Dissection of Diverse Seed Coat Patterns in Cowpea through a Comprehensive GWAS Approach

Plants, Год журнала: 2024, Номер 13(9), С. 1275 - 1275

Опубликована: Май 5, 2024

This study investigates the genetic determinants of seed coat color and pattern variations in cowpea (Vigna unguiculata), employing a genome-wide association approach. Analyzing mapping panel 296 varieties with 110,000 single nucleotide polymorphisms (SNPs), we focused on eight unique patterns: (1) Red (2) Cream seed; (3) White (4) Brown/Tan coat; (5) Pink, (6) Black, (7) Browneye (8) Red/Brown Holstein. Across six GWAS models (GLM, SRM, MLM, MLMM, FarmCPU from GAPIT3, TASSEL5), 13 significant SNP markers were identified led to discovery 23 candidate genes. Among these, four specific genes may play direct role determining pigment. These findings lay foundational basis for future breeding programs aimed at creating aligned consumer preferences market requirements.

Язык: Английский

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Orphan legumes: harnessing their potential for food, nutritional and health security through genetic approaches

Planta, Год журнала: 2022, Номер 256(2)

Опубликована: Июнь 29, 2022

Язык: Английский

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Cowpea Constraints and Breeding in Europe

Plants, Год журнала: 2023, Номер 12(6), С. 1339 - 1339

Опубликована: Март 16, 2023

Cowpea (Vigna unguiculata (L.) Walp.) is a legume with constant rate of cultivation in Southern European countries. Consumer demand for cowpea worldwide rising due to its nutritional content, while Europe constantly attempting reduce the deficit production pulses and invest new, healthy food market products. Although climatic conditions that prevail are not so harsh terms heat drought as tropical climates where mainly cultivated, confronts plethora abiotic biotic stresses yield-limiting factors In this paper, we summarize main constraints breeding methods have been or can be used. A special mention made availability plant genetic resources (PGRs) their potential purposes, aiming promote more sustainable cropping systems shifts become frequent fiercer, environmental degradation expands worldwide.

Язык: Английский

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Combined evaluation of agronomic and quality traits to explore heat germplasm in celery (Apium graveolens L.)

Scientia Horticulturae, Год журнала: 2023, Номер 317, С. 112039 - 112039

Опубликована: Апрель 18, 2023

Язык: Английский

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Genetic Diversity Analysis of Onion (Allium cepa L.) from the Arid Region of Tunisia Using Phenotypic Traits and SSR Markers

Horticulturae, Год журнала: 2023, Номер 9(10), С. 1098 - 1098

Опубликована: Окт. 3, 2023

The present study was carried out to evaluate and characterize, for the first time, genetic potential of a collection onion (Allium cepa L.) local accessions from arid region southern Tunisia. diversity among 135 individuals, belonging 23 accessions, evaluated using microsatellite (SSR) markers phenotypic information. A total 35 alleles were generated with 11 SSRs. polymorphic information content (PIC) value ranged 0.08 0.95, an average PIC 0.43. expected heterozygosity averaged 0.37, observed 0.42. analysis molecular variance (AMOVA) revealed that 79% variation existed within individuals. Structure cluster grouped into two major clusters: landraces pre-breeding lines. For traits evaluated, field trials conducted in different environments. Significant differences shown, most traits, there also significant environmental effect interaction between environment accession. Great variability found all could be exploited create new varieties adapted conditions by selecting appropriate parents hybridization breeding.

Язык: Английский

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Analysis of Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata (L.) Walp) Genotypes Using Single Nucleotide Polymorphism Markers

Plants, Год журнала: 2022, Номер 11(24), С. 3480 - 3480

Опубликована: Дек. 12, 2022

Cowpea (Vigna unguiculata (L.) Walp) is an important legume crop with immense potential for nutritional and food security, income generation, livestock feed in Sub-Saharan Africa. The highly tolerant to heat drought stresses which makes it extremely improving resilience production the face of climate change. This study was carried out assess genetic diversity population structure 90 cowpea accessions using single nucleotide polymorphism (SNP) markers. Out 11,940 SNPs used, 5864 were polymorphic maintained genome analysis. Polymorphic information content (PIC) values ranged from 0.22 0.32 a mean value 0.27. model-based Bayesian STRUCTURE analysis classified into four subpopulations at K = 4, while distance-based cluster grouped three distinct clusters. molecular variance (AMOVA) revealed that 59% 69% total variation attributed among individual populations, respectively, 18% within variations. Furthermore, low heterozygosity high inbreeding coefficient observed this suggests reached acceptable level homozygosity. would serve as reference future selection breeding programs desirable traits systematic conservation these plant resources.

Язык: Английский

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Genetic Diversity Assessment in Grain Legumes

Sustainable development and biodiversity, Год журнала: 2024, Номер unknown, С. 399 - 426

Опубликована: Янв. 1, 2024

Язык: Английский

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Molecular Fingerprinting and Hybridity Authentication in Cowpea Using Single Nucleotide Polymorphism Based Kompetitive Allele-Specific PCR Assay

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Окт. 5, 2021

Optimization of a breeding program for increased genetic gain requires quality assurance (QA) and control (QC) at key phases the process. One vital phase in that QC QA is choice parents successful hybridizations to combine parental attributes create variations. The objective this study was determine diversity confirm hybridity cowpea F 1 progenies using KASP (Kompetitive Allele-Specific PCR)-based single nucleotide polymorphism (SNP) markers. A total 1,436 plants were derived from crossing 220 lines landraces 2 elite sister IT99K-573-1-1 IT99K-573-2-1 as male parents, constituting 225 cross combinations. genotyped with 17 SNP markers via high-throughput genotyping assay. differentiated mean efficiency 37.90% range 3.4–82.8%, revealing unique fingerprints parents. Neighbor-Joining cladogram divided 222 into 3 clusters. Genetic distances between ranged 0 3.74 2.41. Principal component analysis (PCA) depicted considerable overlap more scatters among than s. differentiation s best contributed by 82% As expected, showed significant contrast proportion heterozygous individuals, values 0.02 0.32, respectively. detected true 100% success rate 72% populations. Overall, 79% putative hybrids, 14% selfed plants, 7% undetermined due missing data lack marker demonstrated an effective application KASP-based assay fingerprinting, confirmation hybridity, early detection false plants. results further uncovered need deploy step program.

Язык: Английский

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Diversity of white Guinea yam (Dioscorea rotundataPoir.) cultivars from Benin as revealed by agro-morphological traits and SNP markers

Plant Genetic Resources, Год журнала: 2021, Номер 19(5), С. 437 - 446

Опубликована: Окт. 1, 2021

Abstract White Guinea yam ( Dioscorea rotundata Poir.) is indigenous to West Africa, a region that harbours the crop's tremendous landrace diversity. The knowledge and understanding of local cultivars’ genetic diversity are essential for properly managing resources, conservation, sustainable use their improvement through breeding. This study aimed dissect phenotypic molecular white cultivars from Benin using agro-morphological single nucleotide polymorphism (SNP) markers. Eighty-eight Beninese collected countrywide ethnobotanical survey were phenotyped with 53 traits genotyped 9725 DArT-SNP. Multivariate analysis revealed 30 as most discriminative explained up 80.78% variation. Assessment indices such Shannon–Wiener H ′), inverse Shannon (H.B.), Simpson's λ ) index Pilou evenness J based data depicted moderate in cultivars. Genetic differentiation among country production zones was low due high exchange planting materials farmers different regions. However, there within Hierarchical clusters (HCs) on presence two groups while HCs SNP markers combined identified three groups. Our result provided valuable insights into its proper conservation

Язык: Английский

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Single and multi‐trait genome‐wide association studies identify genomic regions associated with phenological traits in cowpea

Crop Science, Год журнала: 2023, Номер 63(6), С. 3443 - 3456

Опубликована: Авг. 23, 2023

Abstract Understanding the genetic control of traits linked to phenology is essential address strategies and efforts in development resilient cultivars capable adapting climate change. In this study, we performed genome‐wide association studies on a cowpea [ Vigna unguiculata (L.) Walp] mini‐core collection dissect architecture identify genomic regions associated with days flowering, from flowering pod maturity, maturity. Phenotypic data were collected 2 years used test associations 41,533 single‐nucleotide polymorphism markers by single multi‐trait analysis. addition, searched for candidate genes putatively involved phenological within genome‐associated regions. The single‐trait approach identified 28 significant marker‐trait three traits, while 18 associations, both evidencing pleiotropic associations. Five these reported previous studies. Candidate identified, including phosphorylation encoding maternal effect embryo arrest 60, known be maturity respectively. Functional validation will further improve our understanding variation other related crops.

Язык: Английский

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