Plant secondary metabolites against biotic stresses for sustainable crop protection
Biotechnology Advances,
Год журнала:
2025,
Номер
unknown, С. 108520 - 108520
Опубликована: Янв. 1, 2025
Язык: Английский
Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals the Key Genes Involved in Triterpenoid Biosynthesis of Ganoderma lucidum
Journal of Fungi,
Год журнала:
2025,
Номер
11(1), С. 57 - 57
Опубликована: Янв. 13, 2025
Ganoderma
lucidum
is
a
traditional
Chinese
medicinal
fungus,
and
ganoderma
triterpenoids
(GTs)
are
one
of
the
main
bioactive
compounds.
These
compounds
have
various
pharmacological
functions,
including
anti-tumor,
antioxidant,
anti-inflammatory,
liver-protective,
immune-regulating
effects.
However,
manner
in
which
they
accumulate,
their
biosynthesis
mechanisms
remain
unclear.
To
screen
for
genes
that
involved
biosynthetic
pathway
GTs,
this
study
analyzed
differential
metabolites
differentially
expressed
(DEGs)
among
different
growth
stages
G.
lucidum,
primordia
(P),
matured
fruiting
body
(FM),
post-spore
bodies
(FP)
using
targeted
metabolomics
transcriptomics
analysis,
respectively.
The
results
showed
total
699
components
were
detected,
lignans,
terpenoids,
amino
acids
derivatives,
phenolic
acids,
others.
Among
them,
112
types
triterpenes
detected.
Compared
with
primordia,
there
eight
triterpenoids,
three
decreasing
five
increasing
FM
stage.
A
comparison
between
stage
FP
revealed
13
triterpenoids.
analysis
371
DEGs
P_vs_FM
group,
171
down-regulated
200
up-regulated
genes.
In
FM_vs_FP
2567
identified,
1278
1289
Through
transcriptome
correlation
six
TFs
two
CYP450s
significantly
associated
four
triterpenoid
components.
these
positively
or
negatively
correlated
addition,
interestingly,
some
flavonoids
compounds,
been
reported
plants,
also
detected
indicating
it
has
potential
to
be
engineered
into
strain
capable
synthesizing
flavonoid
This
provides
useful
information
about
key
GT
biosynthesis,
but
further
exploration
in-depth
research
needed
better
understand
functions
Язык: Английский
Integrated Transcriptome and Metabolome Analysis Elucidates the Defense Mechanisms of Pumpkin Against Gummy Stem Blight
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2586 - 2586
Опубликована: Март 13, 2025
Gummy
stem
blight
(GSB)
is
a
pervasive
disease
that
causes
considerable
economic
losses
in
cucurbit
crops
and
poses
significant
threat
to
pumpkin
production.
However,
the
molecular
interaction
mechanisms
between
pathogen
remain
largely
unexplored.
In
our
previous
research,
we
isolated
identified
Stagonosporopsis
cucurbitacearum
(Sc)
as
primary
causative
agent
of
Northeast
China.
Through
whole-genome
analysis,
several
pathogenic
genes
associated
with
Sc
infection
pumpkins.
this
study,
performed
comprehensive
comparative
transcriptomic
metabolomic
analysis
unvaccinated
Sc-inoculated
We
observed
distinct
differences
gene
expression
profiles,
these
being
significantly
enriched
pathways
related
plant–pathogen
interactions,
phytohormone
signal
transduction,
metabolic
processes,
including
phenylpropanoid
biosynthesis.
Joint
revealed
biosynthesis
pathway
was
activated
Sc-infected
Notably,
two
metabolites
involved
flavonoid
pathways,
p-coumaric
acid
quercetin,
exhibited
upregulation,
suggesting
their
potential
roles
conferring
resistance
GSB.
These
findings
enhance
understanding
underlying
defense
response
against
GSB
pumpkins
may
provide
valuable
insights
for
developing
strategies
control
disease.
Язык: Английский
Insights into the Role of GhTAT2 Genes in Tyrosine Metabolism and Drought Stress Tolerance in Cotton
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1355 - 1355
Опубликована: Фев. 5, 2025
Gossypium
hirsutum
is
a
key
fiber
crop
that
sensitive
to
environmental
factors,
particularly
drought
stress,
which
can
reduce
boll
size,
increase
flower
shedding,
and
impair
photosynthesis.
The
aminotransferase
(AT)
gene
essential
for
abiotic
stress
tolerance.
A
total
of
3
species
were
analyzed
via
genome-wide
analysis,
the
results
unveiled
103
genes
in
G.
hirsutum,
47
arboreum,
53
raimondii.
Phylogenetic
structure
examination,
motif
subcellular
localization
prediction,
promoter
analysis
revealed
GhAT
be
classified
into
five
main
categories
play
roles
Using
RNA-seq
expression
KEGG
enrichment
GhTAT2,
coexpression
network
was
established,
followed
by
RT-qPCR
identify
hub
genes.
Gh_A13G1261,
Gh_D13G1562,
Gh_D10G1155,
Gh_A10G1320,
Gh_D06G1003
significantly
upregulated
leaf
root
samples
following
treatment,
with
Gh_A13G1261
identified
as
gene.
GhTAT2
considerably
enriched
tyrosine,
cysteine,
methionine,
phenylalanine
metabolism
isoquinoline
alkaloid,
tryptophan,
tropane,
piperidine,
pyridine
alkaloid
biosynthesis.
Under
manifested
significant
upregulation
amino
acids
such
L-DOPA,
L-alanine,
L-serine,
L-homoserine,
L-methionine,
L-cysteine,
whereas
metabolites
maleic
acid,
p-coumaric
quinic
vanillin,
hyoscyamine
downregulated.
Silencing
affected
shoot
fresh
weights
plants
compared
those
wild-type
under
conditions.
VIGS-treated
seedlings
lower
than
both
positive
control
plants,
indicating
silencing
increases
sensitivity
stress.
In
summary,
this
thorough
family
lays
groundwork
detailed
study
members,
specific
focus
on
their
contributions
Язык: Английский
Optimizing Cucumber (Cucumis sativus L.) Fruit Metabolomics Under Elevated CO2 and High-Temperature Stress in the Greenhouse
Horticulturae,
Год журнала:
2024,
Номер
11(1), С. 10 - 10
Опубликована: Дек. 26, 2024
Elevated
carbon
dioxide
concentrations
can
mitigate
the
significant
threats
of
high-temperature
stress
to
yield
and
quality
cucumber
fruit
during
summer.
This
study
aims
elucidate
response
mechanisms
metabolomics
under
elevated
CO2
while
also
exploring
potential
benefits
in
mitigating
adverse
effects
high
temperatures.
The
fruits
experimental
material
(Cucumis
sativus
L.,
’Jinyou
35’)
were
grown
soil
conditions
greenhouse.
We
used
untargeted
methods
analyze
varying
temperatures
(normal
25
35
°C
45
°C)
(400
±
20
µmol/mol
1200
µmol/mol)
on
morpho-physiological
traits,
yield-associated
metabolomic
profiles
fruits.
results
showed
that,
stress,
altered
27
differential
metabolites,
including
tyramine,
xylitol,
linolenic
acid,
L-asparagine,
α-linolenic
L-phenylalanine.
These
alterations
are
associated
with
metabolic
pathways
alanine,
aspartate,
glutamate,
glutathione,
glyoxylate,
dicarboxylic
acids.
Compared
adding
at
normal
temperatures,
modified
38
vitamin
B6,
L-citrulline,
inositol,
L-aspartic
sucrose,
palmitic
acid.
modifications
linked
galactose
pathway
zeatin
arginine
biosynthetic
pathways.
accumulation
cysteine,
glutamic
glycine
is
essential
form
antioxidant
glutathione;
thus,
a
higher
amino
acid
content
exhibit
an
enhanced
capacity
withstand
severe
stress.
Under
conditions,
adds
complexity
changes
metabolites
within
accumulate
sugars,
organic
acids,
acids
through
metabolism
(map00052),
biosynthesis
(map00220),
glutamate
synthesis
(map00250),
thereby
improving
their
heat
resistance.
Язык: Английский