Optimizing Cucumber (Cucumis sativus L.) Fruit Metabolomics Under Elevated CO2 and High-Temperature Stress in the Greenhouse DOI Creative Commons

Xian Du,

Yang Song,

Lu Pan

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 11(1), P. 10 - 10

Published: Dec. 26, 2024

Elevated carbon dioxide concentrations can mitigate the significant threats of high-temperature stress to yield and quality cucumber fruit during summer. This study aims elucidate response mechanisms metabolomics under elevated CO2 while also exploring potential benefits in mitigating adverse effects high temperatures. The fruits experimental material (Cucumis sativus L., ’Jinyou 35’) were grown soil conditions greenhouse. We used untargeted methods analyze varying temperatures (normal 25 35 °C 45 °C) (400 ± 20 µmol/mol 1200 µmol/mol) on morpho-physiological traits, yield-associated metabolomic profiles fruits. results showed that, stress, altered 27 differential metabolites, including tyramine, xylitol, linolenic acid, L-asparagine, α-linolenic L-phenylalanine. These alterations are associated with metabolic pathways alanine, aspartate, glutamate, glutathione, glyoxylate, dicarboxylic acids. Compared adding at normal temperatures, modified 38 vitamin B6, L-citrulline, inositol, L-aspartic sucrose, palmitic acid. modifications linked galactose pathway zeatin arginine biosynthetic pathways. accumulation cysteine, glutamic glycine is essential form antioxidant glutathione; thus, a higher amino acid content exhibit an enhanced capacity withstand severe stress. Under conditions, adds complexity changes metabolites within accumulate sugars, organic acids, acids through metabolism (map00052), biosynthesis (map00220), glutamate synthesis (map00250), thereby improving their heat resistance.

Language: Английский

Plant secondary metabolites against biotic stresses for sustainable crop protection DOI
Tanzim Jahan, Md. Nurul Huda, Kaixuan Zhang

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108520 - 108520

Published: Jan. 1, 2025

Language: Английский

Citations

2
Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals the Key Genes Involved in Triterpenoid Biosynthesis of Ganoderma lucidum DOI Creative Commons
Xiaolan Xu, Chunxia Li, Feilun Wu

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 57 - 57

Published: Jan. 13, 2025

Ganoderma lucidum is a traditional Chinese medicinal fungus, and ganoderma triterpenoids (GTs) are one of the main bioactive compounds. These compounds have various pharmacological functions, including anti-tumor, antioxidant, anti-inflammatory, liver-protective, immune-regulating effects. However, manner in which they accumulate, their biosynthesis mechanisms remain unclear. To screen for genes that involved biosynthetic pathway GTs, this study analyzed differential metabolites differentially expressed (DEGs) among different growth stages G. lucidum, primordia (P), matured fruiting body (FM), post-spore bodies (FP) using targeted metabolomics transcriptomics analysis, respectively. The results showed total 699 components were detected, lignans, terpenoids, amino acids derivatives, phenolic acids, others. Among them, 112 types triterpenes detected. Compared with primordia, there eight triterpenoids, three decreasing five increasing FM stage. A comparison between stage FP revealed 13 triterpenoids. analysis 371 DEGs P_vs_FM group, 171 down-regulated 200 up-regulated genes. In FM_vs_FP 2567 identified, 1278 1289 Through transcriptome correlation six TFs two CYP450s significantly associated four triterpenoid components. these positively or negatively correlated addition, interestingly, some flavonoids compounds, been reported plants, also detected indicating it has potential to be engineered into strain capable synthesizing flavonoid This provides useful information about key GT biosynthesis, but further exploration in-depth research needed better understand functions

Language: Английский

Citations

0
Integrated Transcriptome and Metabolome Analysis Elucidates the Defense Mechanisms of Pumpkin Against Gummy Stem Blight DOI Open Access
Qian Zhao, Liyan Zhang,

Weibo Han

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2586 - 2586

Published: March 13, 2025

Gummy stem blight (GSB) is a pervasive disease that causes considerable economic losses in cucurbit crops and poses significant threat to pumpkin production. However, the molecular interaction mechanisms between pathogen remain largely unexplored. In our previous research, we isolated identified Stagonosporopsis cucurbitacearum (Sc) as primary causative agent of Northeast China. Through whole-genome analysis, several pathogenic genes associated with Sc infection pumpkins. this study, performed comprehensive comparative transcriptomic metabolomic analysis unvaccinated Sc-inoculated We observed distinct differences gene expression profiles, these being significantly enriched pathways related plant–pathogen interactions, phytohormone signal transduction, metabolic processes, including phenylpropanoid biosynthesis. Joint revealed biosynthesis pathway was activated Sc-infected Notably, two metabolites involved flavonoid pathways, p-coumaric acid quercetin, exhibited upregulation, suggesting their potential roles conferring resistance GSB. These findings enhance understanding underlying defense response against GSB pumpkins may provide valuable insights for developing strategies control disease.

Language: Английский

Citations

0
Insights into the Role of GhTAT2 Genes in Tyrosine Metabolism and Drought Stress Tolerance in Cotton DOI Open Access
Teame Gereziher Mehari, Jun Tang,

Haijing Gu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1355 - 1355

Published: Feb. 5, 2025

Gossypium hirsutum is a key fiber crop that sensitive to environmental factors, particularly drought stress, which can reduce boll size, increase flower shedding, and impair photosynthesis. The aminotransferase (AT) gene essential for abiotic stress tolerance. A total of 3 species were analyzed via genome-wide analysis, the results unveiled 103 genes in G. hirsutum, 47 arboreum, 53 raimondii. Phylogenetic structure examination, motif subcellular localization prediction, promoter analysis revealed GhAT be classified into five main categories play roles Using RNA-seq expression KEGG enrichment GhTAT2, coexpression network was established, followed by RT-qPCR identify hub genes. Gh_A13G1261, Gh_D13G1562, Gh_D10G1155, Gh_A10G1320, Gh_D06G1003 significantly upregulated leaf root samples following treatment, with Gh_A13G1261 identified as gene. GhTAT2 considerably enriched tyrosine, cysteine, methionine, phenylalanine metabolism isoquinoline alkaloid, tryptophan, tropane, piperidine, pyridine alkaloid biosynthesis. Under manifested significant upregulation amino acids such L-DOPA, L-alanine, L-serine, L-homoserine, L-methionine, L-cysteine, whereas metabolites maleic acid, p-coumaric quinic vanillin, hyoscyamine downregulated. Silencing affected shoot fresh weights plants compared those wild-type under conditions. VIGS-treated seedlings lower than both positive control plants, indicating silencing increases sensitivity stress. In summary, this thorough family lays groundwork detailed study members, specific focus on their contributions

Language: Английский

Citations

0
Optimizing Cucumber (Cucumis sativus L.) Fruit Metabolomics Under Elevated CO2 and High-Temperature Stress in the Greenhouse DOI Creative Commons

Xian Du,

Yang Song,

Lu Pan

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 11(1), P. 10 - 10

Published: Dec. 26, 2024

Elevated carbon dioxide concentrations can mitigate the significant threats of high-temperature stress to yield and quality cucumber fruit during summer. This study aims elucidate response mechanisms metabolomics under elevated CO2 while also exploring potential benefits in mitigating adverse effects high temperatures. The fruits experimental material (Cucumis sativus L., ’Jinyou 35’) were grown soil conditions greenhouse. We used untargeted methods analyze varying temperatures (normal 25 35 °C 45 °C) (400 ± 20 µmol/mol 1200 µmol/mol) on morpho-physiological traits, yield-associated metabolomic profiles fruits. results showed that, stress, altered 27 differential metabolites, including tyramine, xylitol, linolenic acid, L-asparagine, α-linolenic L-phenylalanine. These alterations are associated with metabolic pathways alanine, aspartate, glutamate, glutathione, glyoxylate, dicarboxylic acids. Compared adding at normal temperatures, modified 38 vitamin B6, L-citrulline, inositol, L-aspartic sucrose, palmitic acid. modifications linked galactose pathway zeatin arginine biosynthetic pathways. accumulation cysteine, glutamic glycine is essential form antioxidant glutathione; thus, a higher amino acid content exhibit an enhanced capacity withstand severe stress. Under conditions, adds complexity changes metabolites within accumulate sugars, organic acids, acids through metabolism (map00052), biosynthesis (map00220), glutamate synthesis (map00250), thereby improving their heat resistance.

Language: Английский

Citations

0