Do Melanocytes Have a Role in Controlling Epidermal Bacterial Colonisation and the Skin Microbiome? DOI Creative Commons

Omera Bi,

David Caballero‐Lima, Stephen Sikkink

и другие.

Experimental Dermatology, Год журнала: 2025, Номер 34(3)

Опубликована: Март 1, 2025

In addition to producing melanin protect epidermal keratinocytes against DNA damage, melanocytes may have important roles in strengthening innate immunity pathogens. We developed a functional, pigmented, human full-thickness 3D skin equivalent determine whether the presence of impacts bacterial growth and regulates expression genes involved immune response. introduced primary construct 3-cell with dermal fibroblasts keratinocytes. Immunohistochemistry verified appropriate ratio spatial organisation melanocytes. Alpha-MSH induced melanogenesis, confirming an physiological compared this 2-cell version without response inoculation 3 species bacteria: Staphylococcus epidermidis, Corynebacterium striatum, Cutibacterium acnes. There was significant decrease colonisation bacteria equivalents containing functional increased immune-response (S100A9, DEFB4A, IL-4R) following microorganism exposure; however, there were marked differences between unpigmented pigmented equivalents. This physiologically relevant 3D-skin opens up new avenues for studying complex pigmentation disorders, melanoma, UV as well rapidly evolving field microbiome balance commensal pathogenic species.

Язык: Английский

Microbiome Dynamics: A Paradigm Shift in Combatting Infectious Diseases DOI Open Access
Mohamed Kamel,

Sami Aleya,

Majed Alsubih

и другие.

Journal of Personalized Medicine, Год журнала: 2024, Номер 14(2), С. 217 - 217

Опубликована: Фев. 18, 2024

Infectious diseases have long posed a significant threat to global health and require constant innovation in treatment approaches. However, recent groundbreaking research has shed light on previously overlooked player the pathogenesis of disease-the human microbiome. This review article addresses intricate relationship between microbiome infectious unravels its role as crucial mediator host-pathogen interactions. We explore remarkable potential harnessing this dynamic ecosystem develop innovative strategies that could revolutionize management diseases. By exploring latest advances emerging trends, aims provide new perspective combating by targeting

Язык: Английский

Процитировано

23
Tumor microbiome – an integral part of the tumor microenvironment DOI Creative Commons
Sona Ciernikova, Aneta Sevcikova,

Viola Stevurkova

и другие.

Frontiers in Oncology, Год журнала: 2022, Номер 12

Опубликована: Ноя. 24, 2022

The tumor microenvironment (TME) plays a significant role in progression and cancer cell survival. Besides malignant cells non-malignant components, including immune cells, elements of the extracellular matrix, stromal endothelial microbiome is considered to be an integral part TME. Mounting evidence from preclinical clinical studies evaluated presence type-specific intratumoral bacteria. Differences composition between cancerous tissues benign controls suggest importance microbiome-based approach. Complex host-microbiota crosstalk within TME affects biology via regulation oncogenic pathways, response modulation, interaction with microbiota-derived metabolites. Significantly, involvement tumor-associated microbiota drug metabolism highlights therapeutic implications. This review aims summarize current knowledge about emerging various types solid malignancies. utility treatment also discussed. Moreover, we provide overview trials evaluating patients. research focusing on communication gut microbiomes may bring new opportunities for targeting increase efficacy improve patient outcomes.

Язык: Английский

Процитировано

40
Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy DOI Creative Commons
Natalia Szóstak, Luiza Handschuh, Anna Samelak-Czajka

и другие.

Cancer Immunology Research, Год журнала: 2024, Номер 12(4), С. 427 - 439

Опубликована: Фев. 5, 2024

Abstract Recent research indicates that gut microbiota may be vital in the advancement of melanoma. In this study, we found melanoma patients exhibited a distinct mycobiota structure compared with healthy participants. Candida albicans, dubliniensis, and Neurospora crassa were more abundant samples from melanoma, whereas Saccharomyces cerevisiae Debaryomyces hansenii less abundant. During anti–PD-1 treatment, relative amount Malassezia restricta C. albicans increased. A higher level paradoxus was associated positive response to Tetrapisispora blattae lack clinical benefits. High levels M. elevated serum lactate dehydrogenase, being overweight linked increased risk progression poorer treatment. Thus, study has revealed melanoma-associated mycobiome dysbiosis, characterized by altered fungal composition fungi species progression, identifying role for progression.

Язык: Английский

Процитировано

9
Enhanced skin cancer diagnosis using optimized CNN architecture and checkpoints for automated dermatological lesion classification DOI Creative Commons

M. Mohamed Musthafa,

T R Mahesh, V. Vinoth Kumar

и другие.

BMC Medical Imaging, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 2, 2024

Abstract Skin cancer stands as one of the foremost challenges in oncology, with its early detection being crucial for successful treatment outcomes. Traditional diagnostic methods depend on dermatologist expertise, creating a need more reliable, automated tools. This study explores deep learning, particularly Convolutional Neural Networks (CNNs), to enhance accuracy and efficiency skin diagnosis. Leveraging HAM10000 dataset, comprehensive collection dermatoscopic images encompassing diverse range lesions, this introduces sophisticated CNN model tailored nuanced task lesion classification. The model’s architecture is intricately designed multiple convolutional, pooling, dense layers, aimed at capturing complex visual features lesions. To address challenge class imbalance within an innovative data augmentation strategy employed, ensuring balanced representation each category during training. Furthermore, optimized layer configuration augmentation, significantly boosting precision detection. learning process using Adam optimizer, parameters fine-tuned over 50 epochs batch size 128 ability discern subtle patterns image data. A Model Checkpoint callback ensures preservation best iteration future use. proposed demonstrates 97.78% notable 97.9%, recall F2 score 97.8%, underscoring potential robust tool classification cancer, thereby supporting clinical decision-making contributing improved patient outcomes dermatology.

Язык: Английский

Процитировано

8
Gut Microbiota–Tumor Microenvironment Interactions: Mechanisms and Clinical Implications for Immune Checkpoint Inhibitor Efficacy in Cancer DOI Creative Commons

Sawsan Sudqi Said,

Wisam Nabeel Ibrahim

Cancer Management and Research, Год журнала: 2025, Номер Volume 17, С. 171 - 192

Опубликована: Янв. 1, 2025

Cancer immunotherapy has transformed cancer treatment in recent years, with immune checkpoint inhibitors (ICIs) emerging as a key therapeutic approach. ICIs work by inhibiting the mechanisms that allow tumors to evade detection. Although have shown promising results, especially solid tumors, patient responses vary widely due multiple intrinsic and extrinsic factors within tumor microenvironment. Emerging evidence suggests gut microbiota plays pivotal role modulating at site may even influence outcomes patients receiving ICIs. This review explores complex interactions between microenvironment, examining how these could impact effectiveness of ICI therapy. Furthermore, we discuss dysbiosis, an imbalance composition, contribute resistance ICIs, highlight microbiota-targeted strategies potentially overcome this challenge. Additionally, studies investigating diagnostic potential profiles patients, considering microbial markers might aid early detection stratification By integrating insights from preclinical clinical studies, aim shed light on microbiome modulation adjunct tool, paving way for personalized approaches optimize outcomes.

Язык: Английский

Процитировано

1
Probiotics and gut microbiota modulation: implications for skin health and disease management DOI

Elahe Parhizkar,

Parisa Vosough,

Maryam Baneshi

и другие.

Archives of Microbiology, Год журнала: 2025, Номер 207(3)

Опубликована: Фев. 23, 2025

Язык: Английский

Процитировано

1
Determinants of resistance and response to melanoma therapy DOI

Bailey M. Robertson,

Mitchell E. Fane, Ashani T. Weeraratna

и другие.

Nature Cancer, Год журнала: 2024, Номер 5(7), С. 964 - 982

Опубликована: Июль 17, 2024

Язык: Английский

Процитировано

8
The cutaneous microbiome in skin cancer – A systematic review DOI

Ruojun Ding,

ShengYang Bertrand Lian,

Yew Chong Tam

и другие.

JDDG Journal der Deutschen Dermatologischen Gesellschaft, Год журнала: 2024, Номер 22(2), С. 177 - 184

Опубликована: Янв. 20, 2024

The overall incidence of skin cancer has risen over the past half a decade worldwide and is associated with significant morbidity mortality. Recent advances in molecular testing have allowed us to better characterize microbiome alterations cancer. However, literature specific remain heterogenous scattered. A systematic review was performed identify existing its usefulness providing microbiome-based biomarkers. search databases (PubMed, Medline, EMBASE, GoogleScholar) conducted from June July 2022 accordance PRISMA guidelines. total 1,543 articles were identified, which 16 selected for inclusion (11 on keratinocytes 5 melanoma). Increased Staphylococcus (S.) aureus prevalence decline commensal organisms seen squamous cell carcinoma (SCC) actinic keratosis (AK), compared healthy skin. While melanoma appears be distinct skin, limited data available draw meaningful conclusions. Our summarizes current evidence keratinocyte cancers melanoma. study establishes that these altered this dysbiosis involves both pathogenic organisms.

Язык: Английский

Процитировано

7
Skin Microbiome and Treatment-Related Skin Toxicities in Patients With Cancer: A Mini-Review DOI Creative Commons
Brianna N. Richardson,

Jolinta Lin,

Zachary S. Buchwald

и другие.

Frontiers in Oncology, Год журнала: 2022, Номер 12

Опубликована: Июль 15, 2022

The human skin hosts millions of bacteria, fungi, archaea, and viruses. These microbes play a crucial role in immunological physiological functions, as well the development diseases, including cancer when balance between commensals pathogens is interrupted. Due to linkages inflammation processes microbes, viral links cancer, new theories have supported dysbiotic microbiome plays treatment-related toxicities. This review focuses on its toxicities, particularly from chemotherapy, radiation therapy, immunotherapy. current literature found changes diversity abundance during treatments such lower microbiome, an increased Proteobacteria/Firmicutes ratio, higher pathogenic Staphylococcus aureus . may be associated with severity acute dermatitis, hand-foot syndrome immunotherapy-induced rash. Several clinical guidelines issued potential interventions (e.g., use topical corticosteroids, phototherapy, non-pharmaceutical care products) prevent treat effectiveness these promising alleviating toxicities should further tested among patients.

Язык: Английский

Процитировано

24
The gut microbiome in bullous pemphigoid: implications of the gut-skin axis for disease susceptibility DOI Creative Commons
Xiaolin Liu, Nina van Beek,

Aleksa Čepić

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 10, 2023

Bullous pemphigoid (BP) is an autoimmune blistering disease that primarily affects the elderly. An altered skin microbiota in BP was recently revealed. Accumulating evidence points toward a link between gut and diseases; however, composition of patients remains largely underexplored, with only one pilot study to date, very limited sample size no functional profiling microbiota. To thoroughly investigate function patients, explore possible links conditions microbiota, we here investigated 66 (81.8% firstly diagnosed) suffering from age-, sex-, center-matched controls (CL) non-inflammatory diseases (132 total participants), using 16S rRNA gene shotgun sequencing data. Decreased alpha-diversity overall microbial community observed patients. Similar trends are subclassifications including first diagnoses relapsed cases. Furthermore, observe set disease-associated features, reduced Faecalibacterium prausnitzii greater abundance pathways related gamma-aminobutyric acid (GABA) metabolism Interestingly, F. well-known microbiomarker inflammatory diseases, which has been reported be microbiome atopic dermatitis psoriasis Moreover, GABA plays multiple roles maintaining health, inhibition itching by acting as neurotransmitter, attenuating lesions balancing Th1 Th2 levels, elasticity increasing expression type I collagen. These findings thus suggest alterations present may play role disease, certain key microbes functions contribute dysbiosis activity. Further studies underlying mechanisms gut-skin interaction clearly warranted, could aid development potential therapeutic interventions.

Язык: Английский

Процитировано

15