From Exosome Biogenesis to Absorption: Key Takeaways for Cancer Research DOI Open Access

Nicolas Cheuk Hang Lau,

Judy Wai Ping Yam

Cancers, Год журнала: 2023, Номер 15(7), С. 1992 - 1992

Опубликована: Март 27, 2023

Exosomes are mediators of intercellular communication in normal physiology and diseases. While many studies have emerged on the function exosomal cargoes, questions remain regarding origin these exosomes. The packaging secretion exosomes different contexts modify composition, which may turn impact delivery, uptake cargo recipient cells. A mechanistic understanding exosome biology is therefore crucial to investigating complex biological systems development novel therapeutic approaches. Here, we outline steps biogenesis, including endosome formation, MVB sorting extracellular release, as well absorption, targeting, interaction with cells fate internalized In addition providing a framework dynamics, summarize current evidence major pathways regulatory mechanisms. We also highlight various mechanisms observed cancer point out directions improve study design biology. Further research needed illuminate relationship between biogenesis function, will aid translational applications.

Язык: Английский

Mesenchymal stem cell-derived extracellular vesicles in skin wound healing: roles, opportunities and challenges DOI Creative Commons

Jiayi Ding,

Minjiang Chen, Lingfeng Wu

и другие.

Military Medical Research, Год журнала: 2023, Номер 10(1)

Опубликована: Авг. 17, 2023

Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.

Язык: Английский

Процитировано

101
Exosomes as natural nanocarrier-based drug delivery system: recent insights and future perspectives DOI Open Access

Srijita Sen,

Joyal Xavier,

Nitesh Kumar

и другие.

3 Biotech, Год журнала: 2023, Номер 13(3)

Опубликована: Фев. 27, 2023

Язык: Английский

Процитировано

91
Liquid biopsy in cancer current: status, challenges and future prospects DOI Creative Commons
Liwei Ma, Huiling Guo, Yunxiang Zhao

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Дек. 1, 2024

Cancer has a high mortality rate across the globe, and tissue biopsy remains gold standard for tumor diagnosis due to its level of laboratory standardization, good consistency results, relatively stable samples, accuracy results. However, there are still many limitations drawbacks in application tumor. The emergence liquid provides new ideas early prognosis Compared with biopsy, advantages treatment various types cancer, including non-invasive, quickly so on. Currently, detection received widely attention. It is now undergoing rapid progress, it holds significant potential future applications. Around now, biopsies encompass several components such as circulating cells, DNA, exosomes, microRNA, RNA, platelets, endothelial cells. In addition, advances identification indicators have significantly enhanced possibility utilizing clinical settings. this review, we will discuss application, challenges some common tumors from perspective diverse systems tumors, look forward development prospects field cancer treatment.

Язык: Английский

Процитировано

57
Programming assembly of biomimetic exosomes: An emerging theranostic nanomedicine platform DOI Creative Commons
Xiao Xu, Limei Xu, Caining Wen

и другие.

Materials Today Bio, Год журнала: 2023, Номер 22, С. 100760 - 100760

Опубликована: Авг. 11, 2023

Exosomes have emerged as a promising cell-free therapeutic approach. However, challenges in large-scale production, quality control, and heterogeneity must be overcome before they can used clinically. Biomimetic exosomes containing key components of natural been assembled through extrusion, artificial synthesis, liposome fusion to address these limitations. These exosome-mimetics (EMs) possess similar morphology function but provide higher yields, faster size compared conventional exosomes. This article provides an overview the chemical biological properties various synthetic exosome systems, including nanovesicles (NVs), EMs, hybrid We highlight recent advances production applications nanobiotechnology discuss advantages, limitations, potential clinical programming assembly mimetics.

Язык: Английский

Процитировано

54
Extracellular vesicles and their engineering strategies, delivery systems, and biomedical applications DOI
Chunhao Yang,

Yunxin Xue,

Yu Duan

и другие.

Journal of Controlled Release, Год журнала: 2024, Номер 365, С. 1089 - 1123

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

54
Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer DOI Creative Commons
Wei Zheng, Tianchuan Zhu,

Lantian Tang

и другие.

Journal of Translational Medicine, Год журнала: 2023, Номер 21(1)

Опубликована: Июнь 12, 2023

Non-small cell lung cancer (NSCLC) is a worldwide health threat with high annual morbidity and mortality. Chemotherapeutic drugs such as paclitaxel (PTX) have been widely applied clinically. However, systemic toxicity due to the non-specific circulation of PTX often leads multi-organ damage, including liver kidney. Thus, it necessary develop novel strategy enhance targeted antitumor effects PTX.Here, we engineered exosomes derived from T cells expressing chimeric antigen receptor (CAR-Exos), which mesothelin (MSLN)-expressing Lewis (MSLN-LLC) through anti-MSLN single-chain variable fragment (scFv) CAR-Exos. was encapsulated into CAR-Exos (PTX@CAR-Exos) administered via inhalation an orthotopic mouse model.Inhaled PTX@CAR-Exos accumulated within tumor area, reduced size, prolonged survival little toxicity. In addition, reprogrammed microenvironment reversed immunosuppression, attributed infiltrating CD8+ elevated IFN-γ TNF-α levels.Our study provides nanovesicle-based delivery platform promote efficacy chemotherapeutic fewer side effects. This may ameliorate present obstacles clinical treatment cancer.

Язык: Английский

Процитировано

48
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

и другие.

Advanced Science, Год журнала: 2024, Номер 11(30)

Опубликована: Июнь 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Язык: Английский

Процитировано

41
Highly Bioactive MXene-M2-Exosome Nanocomposites Promote Angiogenic Diabetic Wound Repair through Reconstructing High Glucose-Derived Immune Inhibition DOI
Xiaoqi Jiang, Junping Ma,

Kaikai Xue

и другие.

ACS Nano, Год журнала: 2024, Номер 18(5), С. 4269 - 4286

Опубликована: Янв. 25, 2024

The repair of diabetic wounds remains challenging, primarily due to the high-glucose-derived immune inhibition which often leads excessive inflammatory response, impaired angiogenesis, and heightened susceptibility infection. However, means reduce immunosuppression regulate conversion M2 phenotype macrophages under a high-glucose microenvironment using advanced biomaterials for are not yet fully understood. Herein, we report two-dimensional carbide (MXene)-M2 macrophage exosome (Exo) nanohybrids (FM-Exo) promoting wound by overcoming inhibition. FM-Exo showed sustained release macrophage-derived exosomes (M2-Exo) up 7 days exhibited broad-spectrum antibacterial activity. In microenvironment, relative single Exo, could significantly induce optimized M2a/M2c polarization ratio activating PI3K/Akt signaling pathway, proliferation, migration fibroblasts, angiogenic ability endothelial cells. full-thickness model, effectively regulated status promoted their transition phenotype, thereby inhibiting inflammation, angiogenesis through VEGF secretion, improving proper collagen deposition. As result, healing process was accelerated, leading better outcome with reduced scarring. Therefore, this study introduced promising approach address developing bioactive nanomaterials in environment.

Язык: Английский

Процитировано

38
Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade DOI Creative Commons
Saeid Moghassemi, Arezoo Dadashzadeh, Maria João Sousa

и другие.

Bioactive Materials, Год журнала: 2024, Номер 36, С. 126 - 156

Опубликована: Март 2, 2024

Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.

Язык: Английский

Процитировано

35
Biogenesis and delivery of extracellular vesicles: harnessing the power of EVs for diagnostics and therapeutics DOI Creative Commons

Jivin Yu,

Saba Sane, Ji-Eun Kim

и другие.

Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 10

Опубликована: Янв. 3, 2024

Extracellular vesicles (EVs) are membrane-enclosed particles secreted by a variety of cell types. These encapsulate diverse range molecules, including proteins, nucleic acids, lipids, metabolites, and even organelles derived from their parental cells. While EVs have emerged as crucial mediators intercellular communication, they also hold immense potential both biomarkers therapeutic agents for numerous diseases. A thorough understanding EV biogenesis is the development EV-based diagnostic developments since composition can reflect health disease status donor cell. Moreover, when taken up target cells, exert profound effects on gene expression, signaling pathways, cellular behavior, which makes these biomolecules enticing targets interventions. Yet, despite decades research, intricate processes underlying cells subsequent uptake recipient remain poorly understood. In this review, we aim to summarize current insights advancements in mechanisms EVs. By shedding light fundamental governing delivery, review underscores basic mechanistic research pave way developing novel strategies applications.

Язык: Английский

Процитировано

32