Cell investigation., Год журнала: 2024, Номер 1(1), С. 100006 - 100006
Опубликована: Дек. 5, 2024
Язык: Английский
ImmunoTargets and Therapy, Год журнала: 2025, Номер Volume 14, С. 25 - 33
Опубликована: Янв. 1, 2025
Our previous study has demonstrated that high expression of immune checkpoints (ICs) was significantly associated with adverse clinical outcomes in patients acute myeloid leukemia (AML). This aims to investigate the significance alteration IC co-expression for evaluating prognosis AML following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Quantitative real-time PCR (qRT-PCR) data bone marrow (BM) samples from 62 de novo patients, including 37 who received allo-HSCT and 25 chemotherapy only, were used prognostic analysis. High PD-1, PD-L1, PD-L2, CTLA-4, LAG-3 poor overall survival (OS) receiving allo-HSCT, while levels LAG-3, other than not correlated OS chemotherapy. Importantly, PD-L1/CTLA-4 best combination model predicting especially combined minimal residual disease (MRD). ICs BM related outcomes, increasing PD-L1 CTLA-4 might be one biomarkers predict AML.
Язык: Английский
Процитировано
0
International Immunopharmacology, Год журнала: 2025, Номер 149, С. 114215 - 114215
Опубликована: Фев. 3, 2025
Язык: Английский
Процитировано
0
European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177652 - 177652
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Hematology, Год журнала: 2025, Номер 30(1)
Опубликована: Апрель 24, 2025
Shikonin (SHK), extracted from the traditional Chinese herb Lithospermum erythrorhizon, demonstrates a wide range of pharmacological activities. This study aimed to explore role and underlying mechanisms 5-methylcytosine (m5C) RNA methyltransferase NOL1/NOP2/SUN domain (NSUN)2 in acute myelocytic leukemia (AML). To assess cell viability death, we employed Cell Counting Kit-8 propidium iodide staining. Ferroptosis-related markers were evaluated using commercial kits Western blot analysis. The m5C levels ferroptosis-associated mRNAs quantified by methylated immunoprecipitation (MeRIP)-qPCR. specific sites on transferrin receptor (TFRC) mRNA identified through dual-luciferase reporter assay, while interaction between NSUN2 TFRC was investigated (RIP). SHK vivo explored xenografted tumor model. Our findings revealed that significantly reduced induced death ferroptosis HL-60 NB4 cells. Notably, treatment led an upregulation expression. Inhibition reversed effects SHK, restoring reducing ferroptosis. Mechanistically, enhanced expression via m5C-dependent methylation. Overexpression similarly decreased increased ferroptosis, mitigated upon silencing TFRC. In vivo, effectively suppressed growth mice. summary, our demonstrated promoted AML modulating NSUN2-mediated methylation These provided novel insights into potential therapeutic strategies for AML.
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12297 - 12309
Опубликована: Ноя. 1, 2024
T-cell acute lymphoblastic leukemia (T-ALL) is a malignant hematological disease with limited targeted therapy options. Overexpression of B-cell lymphoma/leukemia 11B frequently observed in T-ALL and contributes to leukemogenesis. Knockdown BCL11B inhibits cell proliferation induces apoptosis, making it potential therapeutic target. However, the clinical application siRNA therapies hindered by challenges such as poor delivery efficiency outcomes.
Язык: Английский
Процитировано
1
Cell investigation., Год журнала: 2024, Номер 1(1), С. 100006 - 100006
Опубликована: Дек. 5, 2024
Язык: Английский
Процитировано
1