Cell investigation., Journal Year: 2024, Volume and Issue: 1(1), P. 100006 - 100006
Published: Dec. 5, 2024
Language: Английский
ImmunoTargets and Therapy, Journal Year: 2025, Volume and Issue: Volume 14, P. 25 - 33
Published: Jan. 1, 2025
Our previous study has demonstrated that high expression of immune checkpoints (ICs) was significantly associated with adverse clinical outcomes in patients acute myeloid leukemia (AML). This aims to investigate the significance alteration IC co-expression for evaluating prognosis AML following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Quantitative real-time PCR (qRT-PCR) data bone marrow (BM) samples from 62 de novo patients, including 37 who received allo-HSCT and 25 chemotherapy only, were used prognostic analysis. High PD-1, PD-L1, PD-L2, CTLA-4, LAG-3 poor overall survival (OS) receiving allo-HSCT, while levels LAG-3, other than not correlated OS chemotherapy. Importantly, PD-L1/CTLA-4 best combination model predicting especially combined minimal residual disease (MRD). ICs BM related outcomes, increasing PD-L1 CTLA-4 might be one biomarkers predict AML.
Language: Английский
Citations
0
International Immunopharmacology, Journal Year: 2025, Volume and Issue: 149, P. 114215 - 114215
Published: Feb. 3, 2025
Language: Английский
Citations
0
European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177652 - 177652
Published: April 1, 2025
Language: Английский
Citations
0
Hematology, Journal Year: 2025, Volume and Issue: 30(1)
Published: April 24, 2025
Shikonin (SHK), extracted from the traditional Chinese herb Lithospermum erythrorhizon, demonstrates a wide range of pharmacological activities. This study aimed to explore role and underlying mechanisms 5-methylcytosine (m5C) RNA methyltransferase NOL1/NOP2/SUN domain (NSUN)2 in acute myelocytic leukemia (AML). To assess cell viability death, we employed Cell Counting Kit-8 propidium iodide staining. Ferroptosis-related markers were evaluated using commercial kits Western blot analysis. The m5C levels ferroptosis-associated mRNAs quantified by methylated immunoprecipitation (MeRIP)-qPCR. specific sites on transferrin receptor (TFRC) mRNA identified through dual-luciferase reporter assay, while interaction between NSUN2 TFRC was investigated (RIP). SHK vivo explored xenografted tumor model. Our findings revealed that significantly reduced induced death ferroptosis HL-60 NB4 cells. Notably, treatment led an upregulation expression. Inhibition reversed effects SHK, restoring reducing ferroptosis. Mechanistically, enhanced expression via m5C-dependent methylation. Overexpression similarly decreased increased ferroptosis, mitigated upon silencing TFRC. In vivo, effectively suppressed growth mice. summary, our demonstrated promoted AML modulating NSUN2-mediated methylation These provided novel insights into potential therapeutic strategies for AML.
Language: Английский
Citations
0
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12297 - 12309
Published: Nov. 1, 2024
T-cell acute lymphoblastic leukemia (T-ALL) is a malignant hematological disease with limited targeted therapy options. Overexpression of B-cell lymphoma/leukemia 11B frequently observed in T-ALL and contributes to leukemogenesis. Knockdown BCL11B inhibits cell proliferation induces apoptosis, making it potential therapeutic target. However, the clinical application siRNA therapies hindered by challenges such as poor delivery efficiency outcomes.
Language: Английский
Citations
1
Cell investigation., Journal Year: 2024, Volume and Issue: 1(1), P. 100006 - 100006
Published: Dec. 5, 2024
Language: Английский
Citations
1