Frontiers in Molecular Biosciences,
Год журнала:
2024,
Номер
10
Опубликована: Янв. 5, 2024
The
development
of
methods
able
to
modulate
the
binding
affinity
between
proteins
and
peptides
is
paramount
biotechnological
interest
in
view
a
vast
range
applications
that
imply
designed
polypeptides
capable
impair
or
favour
Protein-Protein
Interactions.
Here,
we
applied
peptide
design
algorithm
based
on
shape
complementarity
optimization
electrostatic
compatibility
provided
first
experimental
vitro
proof
efficacy
algorithm.
Focusing
interaction
SARS-CoV-2
Spike
Receptor-Binding
Domain
(RBD)
human
angiotensin-converting
enzyme
2
(ACE2)
receptor,
extracted
23-residues
long
structurally
mimics
major
interacting
portion
ACE2
receptor
silico
five
mutants
such
with
modulated
affinity.
Remarkably,
KD
measurements,
conducted
using
biolayer
interferometry,
matched
predictions.
Moreover,
investigated
molecular
determinants
govern
variation
through
dynamics
simulation,
by
identifying
mechanisms
driving
different
values
at
single
residue
level.
Finally,
sequence
highest
affinity,
comparison
wild
type
peptide,
was
expressed
as
fusion
protein
H
ferritin
(HFt)
24-mer.
Solution
measurements
performed
latter
constructs
confirmed
still
exhibited
expected
trend,
thereby
enhancing
their
RBD
binding.
Altogether,
these
results
indicate
high
potentiality
this
general
method
developing
potent
high-affinity
vectors
for
hindering/enhancing
protein-protein
associations.
Accounts of Chemical Research,
Год журнала:
2021,
Номер
54(17), С. 3313 - 3325
Опубликована: Авг. 20, 2021
Ferritins
are
spherical
iron
storage
proteins
within
cells
that
composed
of
a
combination
24
subunits
two
types,
heavy-chain
ferritin
(HFn)
and
light-chain
(LFn).
They
autoassemble
naturally
into
hollow
nanocage
with
an
outer
diameter
12
nm
interior
cavity
is
8
in
diameter.
In
recent
years,
the
constantly
emerging
safety
issues
concerns
about
unfavorable
uniformity
indefinite
vivo
behavior
traditional
nanomedicines,
characteristics
native
nanocages,
such
as
unique
structure,
excellent
profile,
definite
behavior,
make
ferritin-based
formulations
uniquely
attractive
for
nanomedicine
development.
To
date,
variety
cargo
molecules,
including
therapeutic
drugs
(e.g.,
cisplatin,
carboplatin,
paclitaxel,
curcumin,
atropine,
quercetin,
gefitinib,
daunomycin,
epirubicin,
doxorubicin,
etc.),
imaging
agents
fluorescence
dyes,
radioisotopes,
MRI
contrast
agents),
nucleic
acids
siRNA
miRNA),
metal
nanoparticles
Fe3O4,
CeO2,
AuPd,
CuS,
CoPt,
FeCo,
Ag,
etc.)
have
been
loaded
nanocages
broad
range
biomedical
applications
from
vitro
biosensing
to
targeted
delivery
molecules
living
systems
aid
modified
targeting
ligands
either
genetically
or
chemically.
We
reported
human
HFn
could
selectively
deliver
large
amount
tumors
vivovia
transferrin
receptor
1
(TfR1)-mediated
tumor-cell-specific
followed
by
rapid
internalization.
By
use
intrinsic
tumor-targeting
property
structure
HFn,
cargo-loaded
developed
biological
analysis,
diagnosis,
medicine
view
property,
lack
immunogenicity,
holds
promise
promote
drugs,
diagnostic
agents,
moieties
multifunctional
nanomedicines.Since
report
we
extensively
explored
ideal
nanocarrier
tumor-targeted
anticancer
inorganic
nanoparticles,
radioisotopes.
particular,
genetic
tools,
also
engineered
recognize
broader
disease
biomarkers.
this
Account,
systematically
review
ferritins
characterizing
their
tumor-binding
understanding
mechanism
kinetics
loading
exploring
applications.
finally
discuss
prospect
become
next-generation
nanomedicines.
expect
physicochemical
will
attract
interest
fundamental
drug
research
offer
new
opportunities
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(44)
Опубликована: Март 14, 2023
Proteins
and
enzymes
are
versatile
biomaterials
for
a
wide
range
of
medical
applications
due
to
their
high
specificity
receptors
substrates,
degradability,
low
toxicity,
overall
good
biocompatibility.
Protein
nanoparticles
formed
by
the
arrangement
several
native
or
modified
proteins
into
nanometer-sized
assemblies.
In
this
review,
we
will
focus
on
artificial
nanoparticle
systems,
where
main
structural
element
not
just
an
encapsulated
payload.
While
under
natural
conditions,
only
certain
form
defined
aggregates
nanoparticles,
chemical
modifications
change
in
physical
environment
can
further
extend
pool
available
building
blocks.
This
allows
assembly
many
globular
even
enzymes.
These
advances
preparation
methods
led
emergence
new
generations
nanosystems
that
beyond
transport
vehicles
diverse
applications,
from
multifunctional
drug
delivery
imaging,
nanocatalysis
protein
therapy.
Journal of Drug Delivery Science and Technology,
Год журнала:
2022,
Номер
70, С. 103256 - 103256
Опубликована: Март 11, 2022
In
this
review,
we
focus
on
recent
advances
in
green
nanotechnology,
providing
details
reliable
synthetic
pathways
towards
biocompatible
and
medically
relevant
gold
radioactive
nanoparticles.
We
cover
a
wide
plethora
of
protocols
that
utilize
nanotechnology
with
in-depth
understanding
what
makes
process
green.
section
2,
provide
full
the
intervention
for
production
various
functionalized
nanoparticles
(AuNPs).
Section
3
focuses
characterization
tools
to
be
used
complete
physicochemical,
size
vitro
before
translating
them
into
potential
vivo
applications.
4
science
applications
diagnostic
imaging.
Specifically,
have
highlighted
importance
chemistry
physics
myriad
imaging
including
X-ray
contrast
agents,
single
photon
emission
computed
tomography
(SPECT)
imaging,
positron-emission
(PET)
optical
coherence
(OCT),
fluorescence
magnetic
resonance
(MRI),
surface
enhanced
Raman
spectroscopy
(SERS),
ultrasound
(US)
5
discusses
latest
drug
delivery,
photothermal
therapy
cancer
therapy.
particular,
discuss
nanotechnological
interventions
how
electron
rich
phytochemicals
epigallocatechin
gallate
(EGCG)
from
tea,
mangiferin
(MGF)
mango
allied
can
utilized
their
dual
roles,
as
reducing
agents
well
rendering
tumor
specificity
due
strong
cell
receptor
avidity—to
produce
specific
molecular
agents.
also
Therapy.
Important
aspects
relating
systemic
toxicity
ways
mitigate
issues
effective
implementation
biomedical
sciences
are
discussed
6.
The
topics
covered
intended
answer
questions
why
should
use
processes
is
role
nanomedicine
domain
human
health
hygiene.
Most
importantly,
critical
analysis
ubiquitous
nanomedicine.
Cancer Nanotechnology,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 17, 2025
Emphasizing
the
significance
of
cancer-associated
fibroblasts
(CAFs),
non-malignant
yet
pivotal
players
within
tumor
microenvironment
(TME),
this
review
illuminates
role
inflammatory
subtype
(iCAF)
as
catalysts
in
cancer
proliferation,
metastasis,
and
therapeutic
resistance.
Given
their
paramount
importance,
targeting
CAFs
emerges
a
robust
strategy
evolving
landscape
immunotherapy.
Nanomaterials,
distinguished
by
unique
features
malleability,
hold
considerable
promise
biomedicine,
especially
precision-oriented
domain
therapy.
Their
aptitude
for
modulating
immune
responses,
amplifying
drug
efficacy
through
precise
delivery,
discerningly
focusing
on
cells
TME
situates
nanomaterials
formidable
tools
to
transcend
boundaries
set
conventional
treatments.
This
scrutinizes
convoluted
interplay
among
CAFs,
cells,
TME.
It
further
showcases
widely
utilized
management.
We
underscore
potential
nanoscale
delivery
systems
directed
at
underscoring
transformative
power
revolutionizing
therapies,
enhancing
precision,
culminating
improved
patient
outcomes.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Фев. 25, 2025
Nanocarrier
drug
delivery
systems
(NDDS)
have
gained
momentum
in
the
field
of
anticancer
or
nucleic
acid
due
to
their
capacity
aggrandize
targeting
efficacy
and
therapeutic
outcomes
encapsulated
drugs.
A
disadvantage
NDDS
is
that
repeated
administrations
often
encounter
an
obstacle
known
as
"accelerated
blood
clearance
(ABC)
phenomenon".
This
phenomenon
results
rapid
secondary
dose
from
bloodstream
markedly
augmented
liver
accumulation,
which
substantially
undermines
accurate
drugs
effect
NDDS.
Nevertheless,
underlying
mechanism
this
has
not
been
elucidated
there
currently
no
effective
method
for
its
eradication.
In
light
above,
aim
review
provide
a
comprehensive
summary
potential
countermeasures
ABC
phenomenon,
with
view
rejuvenating
both
slow-release
property
expectation
clinic.
paper,
we
innovatively
introduce
pharmacokinetic
further
elucidate
occurrence
after
discussing
immunological
mechanism,
provides
new
direction
expanding
mechanistic
study
phenomenon.
Whereafter,
conducted
critical
conclusion
strategies
suppression
prevention
terms
physical
structural
properties,
PEG-lipid
derivatives,
dosage
regimen
substances
nanoformulations,
particularly
covering
some
novel
high-performance
nanomaterials
mixed
modification
methods.
Alternatively,
propose
promising
strategy
applying
characteristics
significantly
elevated
hepatic
accumulation
activated
CYP3A1
profile
associated
are
proved
be
conducive
enhancing
treatment
hepatocellular
carcinoma.
Collectively,
instructive
surmounting
wielding
advancing
clinical
applications
translations
