Highly integrated, self-powered and activatable bipedal DNA nanowalker for imaging of base excision repair in living cells

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 18, 2024

Язык: Английский

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Advancements in Functional Tetrahedral DNA Nanostructures for Multi-biomarker Biosensing: Applications in Disease Diagnosis, Food Safety, and Environmental Monitoring

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101486 - 101486

Опубликована: Янв. 21, 2025

Язык: Английский

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Smart Health Monitoring: Review of Electrochemical Biosensors for Cortisol Monitoring

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Abstract Cortisol, also known as the stress hormone, is a crucial corticosteroid hormone that significantly increases secretion in human body when facing notable stress. Monitoring cortisol levels for personal management and diagnosis treatment of certain diseases. Electrochemical biosensors combine efficient sensitivity electrochemical technology with high specificity biological recognition processes, making them widely applicable analysis fluid components. This work outlines working mechanism biosensors, focusing particularly on sensing elements such antibodies, aptamers, molecularly imprinted polymers. It provides detailed explanations operational principles these different elements. summarizes evaluates latest advancements detecting fluids, discussing influence sensor design performance. Subsequently, through comparative various performances, further discusses challenges translating laboratory achievements into practical applications, including enhancing key metrics reusability, reproducibility, long‐term stability, continuous monitoring capability, response time. Finally, it offers insights recommendations achieving real‐time, continuous, biosensors.

Язык: Английский

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Biosensor-based methods for exosome detection with applications to disease diagnosis

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117362 - 117362

Опубликована: Март 1, 2025

Язык: Английский

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Advances in electrochemical biosensors for the detection of tumor-derived exosomes

Frontiers in Chemistry, Год журнала: 2025, Номер 13

Опубликована: Март 26, 2025

Exosomes, released from diverse cells as nanoscale lipid bilayer vesicles, mediate intercellular communication and participate in various physiological pathological processes. Thereinto, tumor-derived exosomes (T-EXOs) with molecular cargoes of parent tumor act attractive biomarkers for liquid biopsy. The amount T-EXOs their levels contained specific proteins nucleic acids are closely associated cancer burden classification. Nevertheless, the size relatively low abundance exosomes, well complex body matrix pose daunting challenges efficient isolation sensitive detection T-EXOs. Biosensing fast, convenient accurate method, has been widely employed over past decades. Among them, electrochemical sensors can sensitively detect by measuring change electrical signal caused oxidation or reduction at working electrode surface. This review aims to summarize recent advance biosensors quantification, protein RNA analysis exosomes. Further, future perspectives exosome-based biopsy have discussed.

Язык: Английский

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Integrating All-rounder TiO₂ Accelerated Electrochemiluminescence with Dual-Quenching PDA@COF Probes for Sensitive Quantification and Protein Profiling of Tumorous Exosomes

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(45), С. 61692 - 61702

Опубликована: Ноя. 4, 2024

Exosomes have been perceived as promising biomarkers for noninvasive cancer diagnosis and treatment monitoring. However, the sensitive accurate quantification phenotyping of exosomes remains challenging. Herein, a versatile electrochemiluminescence (ECL) aptasensor was proposed analysis tumorous exosomes. Specifically, ternary nanohybrid (Ru-HAuTiO2), by covalently linking ECL luminophore Ru(dcbpy)32+ with gold nanoparticles (AuNPs)-decorated hollow urchin-like TiO2 (HTiO2), ingeniously designed highly luminescent self-enhanced nanoemitter. Notably, porous HTiO2 played an "all-rounder" role, including carrier luminophores AuNPs, coreaction accelerator, specific exosome capturing scaffold through Ti–phosphate coordination interaction. On other hand, polydopamine modified covalent organic framework (PDA@COF) employed quencher to remarkably attenuate Ru-HAuTiO2 dual-quenching mechanism, further labeled aptamer (Apt) exosomal surface protein. Based on forming Ru-HAuTiO2/exosome/Apt-PDA@COF sandwich structure electrode, "signal on–off" platform constructed, realizing detection within range 3.1 × 103 particles/mL 1 108 low limit 1.41 particles/mL, achieving phenotypic profiling proteins different This work provides alternative method

Язык: Английский

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From biogenesis to aptasensors: advancements in analysis for tumor-derived extracellular vesicles research

Theranostics, Год журнала: 2024, Номер 14(10), С. 4161 - 4183

Опубликована: Янв. 1, 2024

Extracellular vesicles (EVs) are enclosed by a nanoscale phospholipid bilayer membrane and typically range in size from 30 to 200 nm.They contain high concentration of specific proteins, nucleic acids, lipids, reflecting but not identical the composition parent cell.The inherent characteristics variety EVs give them extensive unique advantages field cancer identification treatment.Recently, have been recognized as potential tumor markers for detection cancer.Aptamers, which molecules single-stranded DNA or RNA, demonstrate remarkable specificity affinity their targets adopting distinct tertiary structures.Aptamers offer various over protein counterparts, such reduced immunogenicity, ability convenient large-scale synthesis, straightforward chemical modification.In this review, we summarized biogenesis, sample collection, isolation, storage characterization, finally provided comprehensive survey analysis techniques that based on aptamers.

Язык: Английский

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Solid dielectric electrochemical polishing of 3D-printed parts: Performance and mechanisms

International Journal of Mechanical Sciences, Год журнала: 2024, Номер unknown, С. 109822 - 109822

Опубликована: Ноя. 1, 2024

Язык: Английский

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Exosomes in esophageal cancer: a promising frontier for liquid biopsy in diagnosis and therapeutic monitoring

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Дек. 17, 2024

Esophageal cancer is a common and lethal digestive system malignancy, both treatment efficacy patient survival rates face significant challenges. In recent years, exosomes have emerged as crucial mediators of intercellular communication, demonstrating tremendous clinical potential, particularly in the diagnosis, treatment, prognostic evaluation esophageal cancer. These not only serve biomarkers for early diagnosis prognosis but also modulate tumor growth, metastasis, drug resistance by delivering bioactive molecules. Importantly, can act carriers cancer-related therapeutic agents, optimizing gene therapy strategies to enhance while reducing toxicity side effects. Despite facing challenges applications such purification, enrichment, standardization analytical methods, maintain broad prospects application with potential significantly improve outcomes quality life. This review focuses on innovative role cancer, exploring their value safety disease monitoring assessment response. Furthermore, this study outlines limitations transitioning exosome research from basic studies applications, well solutions future directions address these obstacles.

Язык: Английский

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Exploring the Tumor Microenvironment in Oral Squamous Cell Carcinoma: Implications for Novel Therapeutic Approaches

Oral Oncology Reports, Год журнала: 2024, Номер unknown, С. 100666 - 100666

Опубликована: Окт. 1, 2024

Язык: Английский

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