Comparison of nanovesicles derived from Panax notoginseng at different size: physical properties, composition, and bioactivity DOI Creative Commons

Xiaohang Chen,

Liyu Huang,

Mengyuan Zhang

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 22, 2024

Aim Plant-derived nanovesicles have emerged as potential agents for combating tumors. In this study, we investigated the inhibitory effects of Panax notoginseng -derived (PnNVs) on proliferation and migration squamous cell carcinoma. Additionally, explored relationship between plant tuber size physical properties, composition bioactivity these nanovesicles. Methods We isolated PnNVs from tubers varying sizes: small-sized (s_PnNVs), medium-sized (m_PnNVs) large-sized (l_PnNVs), evaluated size, potential, morphology. Cellular uptake efficiency was assessed using confocal microscopy flow cytometry. The ability different to inhibit oral carcinoma cells plate cloning, CCK8 assay, scratch healing assay. Off-target metabolomics used compare metabolic compounds PnNVs. Results Our findings revealed that s_PnNVs exhibited lower but had highest cellular efficiency, whereas m_PnNVs were characterized by smallest lowest efficiency. Notably, demonstrated most effective inhibition growth migration. Compositional analyses showed rich in proteins contained levels RNA, with l_PnNVs having protein content. Furthermore, untargeted analysis a significant increase expression specific antitumour-related metabolites compared l_PnNVs. Conclusion Overall, our results underscore influence which they are derived, emphasizing its importance experimental design study reproducibility.

Язык: Английский

Exosomes: A Promising Drug Delivery Tool in Hepatic Drug Delivery DOI
Neha Kanojia,

Komal Thapa,

Thakur Gurjeet Singh

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106761 - 106761

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Acid-responsive aggregated carrot-derived nanoantioxidants alleviate oxidative stress and restore osteoblast activity DOI Creative Commons
Jiao Peng, Rongyan Liu, Junyi Xu

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 12, 2025

Excessive generation of reactive oxygen species is a hallmark the osteoporotic bone microenvironment, which leads to damage mitochondrial function and deactivation osteoblasts. Fruits vegetables are rich sources antioxidants, play key role in scavenging free radicals maintaining body's homeostasis. Herein, we have developed type vesicle coming from carrots as nanoantioxidants counteract oxidative stress restore vitality osteoblasts for reversing osteoporosis. Nanovesicles derived carrot juice using straightforward extrusion method, resulting stable membrane structures containing various lipids homologous active phytochemicals. can maintain under normal physiological conditions (pH 7.4) transform into aggregates response acidic extracellular pH osteoporosis 4.0). As anticipated, nanovesicles passively target aggregate bone, ease stress, function, promote osteoblastogenesis, reduce loss mice. This work presents first demonstration novel realize long-awaited osteogenesis, contributing exploration brand-new idea

Язык: Английский

Процитировано

0

Engineering Strategies of Plant-Derived Exosome-Like Nanovesicles: Current Knowledge and Future Perspectives DOI Creative Commons
Yuhan Li, Yulong Wang,

Hongrui Zhao

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12793 - 12815

Опубликована: Ноя. 1, 2024

Plant-derived exosome-like nanovesicles (PELNs) from edible plants, isolated by ultracentrifugation, size exclusion chromatography or other methods, were proved to contain a variety of biologically active and therapeutically specific components. Recently, investigations in the field PELN-based biomedicine have been conducted, which positioned those as promising tools for prevention treatment several diseases, with their natural origin potentially offering superior biocompatibility bioavailability. However, inadequate targeting limited therapeutic effects constrain utility clinical translation PELNs. Thus, strategies aiming at bridging gap engineering PELNs great interest. Those approaches include membrane hybridization, physical chemical surface functionalization encapsulation payloads. Herein, we provide comprehensive overview biogenesis composition, isolation purification methods characterization PELNs, well functions. Current knowledge on construction biomedical application engineered reviewed. Additionally, future directions perspectives this discussed order further enrich expand prospects

Язык: Английский

Процитировано

1

Comparison of nanovesicles derived from Panax notoginseng at different size: physical properties, composition, and bioactivity DOI Creative Commons

Xiaohang Chen,

Liyu Huang,

Mengyuan Zhang

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 22, 2024

Aim Plant-derived nanovesicles have emerged as potential agents for combating tumors. In this study, we investigated the inhibitory effects of Panax notoginseng -derived (PnNVs) on proliferation and migration squamous cell carcinoma. Additionally, explored relationship between plant tuber size physical properties, composition bioactivity these nanovesicles. Methods We isolated PnNVs from tubers varying sizes: small-sized (s_PnNVs), medium-sized (m_PnNVs) large-sized (l_PnNVs), evaluated size, potential, morphology. Cellular uptake efficiency was assessed using confocal microscopy flow cytometry. The ability different to inhibit oral carcinoma cells plate cloning, CCK8 assay, scratch healing assay. Off-target metabolomics used compare metabolic compounds PnNVs. Results Our findings revealed that s_PnNVs exhibited lower but had highest cellular efficiency, whereas m_PnNVs were characterized by smallest lowest efficiency. Notably, demonstrated most effective inhibition growth migration. Compositional analyses showed rich in proteins contained levels RNA, with l_PnNVs having protein content. Furthermore, untargeted analysis a significant increase expression specific antitumour-related metabolites compared l_PnNVs. Conclusion Overall, our results underscore influence which they are derived, emphasizing its importance experimental design study reproducibility.

Язык: Английский

Процитировано

0