Advanced Healthcare Materials,
Год журнала:
2024,
Номер
14(4)
Опубликована: Дек. 23, 2024
Intra-articular
glucocorticoid
injections
are
effective
in
controlling
inflammation
and
pain
arthritides
but
restricted
by
short
duration
of
action
risk
joint
degeneration.
Controlled
drug
release
using
biocompatible
hydrogels
offers
a
unique
solution,
limitations
situ
gelation
restrict
their
application.
Gellan
sheared
(GSHs)
retain
the
advantages
hydrogels,
however
microstructures
lend
themselves
to
intra-articular
application
-
capable
shear
thinning
under
force
restructuring
at
rest
enhance
residence.
This
study
examined
GSHs
for
extended
delivery
prednisolone
(10
mg
mL-1);
demonstrating
links
between
material
mechanics,
steroid
release,
preclinical
assessment
efficacy
synoviocyte
culture
transgenic(TNF)197Gkl
(TNFtg)
murine
model
arthritis.
demonstrated
sustained
with
typical
Fickian
profiles
over
18
days.
Moreover,
systems
showed
good
stability
culture,
inherent
cell-compatibility
suppression
inflammatory
activation.
In
TNFtg
animals,
suppressed
synovitis
(70.08%,
p
<
0.05),
pannus
formation
(45.01%,
increased
articular
cartilage
(82.23%,
0.05)
relative
vehicle
controls.
The
profile
from
injectable
GSH
formulations
holds
promise
treatment
management
such
as
rheumatoid
osteoarthritis,
representing
step-change
suppress
long-term
inflammation.
Journal of Extracellular Vesicles,
Год журнала:
2025,
Номер
14(3)
Опубликована: Март 1, 2025
ABSTRACT
Mesenchymal
stem/stromal
cells
(MSCs)
are
a
valuable
source
of
paracrine
factors,
as
they
have
remarkable
secretory
capacity,
and
there
is
sizeable
knowledge
base
to
develop
industrial
clinical
production
protocols.
Promising
cell‐free
approaches
for
tissue
regeneration
immunomodulation
driving
research
towards
secretome
applications,
among
which
extracellular
vesicles
(EVs)
steadily
gaining
attention.
However,
the
manufacturing
application
EVs
limited
by
insufficient
yields,
gaps,
low
standardization.
Facing
these
limitations,
hydrogels
represent
versatile
three‐dimensional
(3D)
culture
platform
that
can
incorporate
matrix
(ECM)
components
mimic
natural
stem
cell
environment
in
vitro;
via
niche‐mimicking
properties,
regulate
MSCs’
morphology,
adhesion,
proliferation,
differentiation
secretion
capacities.
impact
hydrogel's
architectural,
biochemical
biomechanical
properties
on
remains
poorly
understood,
field
still
its
infancy
interdependency
parameters
compromises
comparability
studies.
Therefore,
this
review
summarizes
discusses
reported
effects
hydrogel
encapsulation
MSC‐EVs.
Considering
cell‐material
interactions
overall
activity
MSCs,
we
identify
persistent
challenges
from
standardization
process
control,
outline
future
paths
research,
such
synergic
use
bioreactors
enhance
MSC‐EV
generation.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 24, 2025
Exosomes-loaded
hydrogels
have
potential
value
in
wound
treatment.
Current
studies
focus
on
improving
hydrogels'
biocompatibility
and
optimizing
different
stem
cell-derived
exosomes
for
better
therapeutic
effect.
Herein,
we
present
a
novel
biocompatible
recombinant
human
collagen
(RHC)
hydrogel
loading
with
MSCs-derived
promoting
healing.
We
modify
the
RHC
methacrylate
anhydride
(MA)
at
optimal
concentration,
generating
(RHCMA)
ideal
physiochemical
properties
exosome
delivery
(MSC-exos@RHCMA).
Exosomes
derived
from
adipose-derived
MSCs
(ADSC-exos),
bone
marrow-derived
(BMSC-exos)
umbilical
cord
(ucMSC-exos)
are
harvested
culture
supernatants
loaded
into
RHCMA,
respectively.
These
three
systems
exhibit
desired
sustained
release
features,
can
significantly
improve
cell
proliferation
migration.
In
addition,
these
MSC-exos@RHCMAs
show
excellent
performance
treating
wounds
of
rats.
Notably,
demonstrated
that
healing
effect
occurs
best
under
treatment
ucMSC-exos@RHCMA,
following
inflammatory
resolution,
angiogenesis,
formation.
results
would
supply
important
clinical
application
MSC-exos
future.
