Harnessing m1A modification: a new frontier in cancer immunotherapy DOI Creative Commons
Xinru Wang, Xiaoqing Ma, Siyu Chen

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Дек. 2, 2024

N1-methyladenosine (m1A) modification is an epigenetic change that occurs on RNA molecules, regulated by a suite of enzymes including methyltransferases (writers), demethylases (erasers), and m1A-recognizing proteins (readers). This significantly impacts the function various biological processes affecting structure, stability, translation, metabolism, gene expression RNA. Thereby, m1A closely associated with occurrence progression cancer. review aims to explore role in tumor immunity. affects immune responses directly regulating cells indirectly modulating microenvironment. Besides, we also discuss implications m1A-mediated metabolic reprogramming its nexus checkpoint inhibitors, unveiling promising avenues for immunotherapeutic intervention. Additionally, m1AScore, established based patterns modification, can be used predict prognosis guide personalized therapy. Our underscores significance as burgeoning frontier cancer biology immuno-oncology, potential revolutionize treatment strategies.

Язык: Английский

Writers, readers, and erasers RNA modifications and drug resistance in cancer DOI Creative Commons
Di Chen, Xinyu Gu,

Yeltai Nurzat

и другие.

Molecular Cancer, Год журнала: 2024, Номер 23(1)

Опубликована: Авг. 30, 2024

Drug resistance in cancer cells significantly diminishes treatment efficacy, leading to recurrence and metastasis. A critical factor contributing this is the epigenetic alteration of gene expression via RNA modifications, such as N6-methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytosine (m5C), 7-methylguanosine (m7G), pseudouridine (Ψ), adenosine-to-inosine (A-to-I) editing. These modifications are pivotal regulating splicing, translation, transport, degradation, stability. Governed by "writers," "readers," "erasers," impact numerous biological processes progression, including cell proliferation, stemness, autophagy, invasion, apoptosis. Aberrant can lead drug adverse outcomes various cancers. Thus, targeting modification regulators offers a promising strategy for overcoming enhancing efficacy. This review consolidates recent research on role prevalent resistance, with focus m6A, m1A, m5C, m7G, Ψ, A-to-I Additionally, it examines regulatory mechanisms linked underscores existing limitations field.

Язык: Английский

Процитировано

16
RNA modifications in cancer DOI Creative Commons

Han Wu,

Shi Chen, Xiang Li

и другие.

MedComm, Год журнала: 2025, Номер 6(1)

Опубликована: Янв. 1, 2025

Abstract RNA modifications are emerging as critical cancer regulators that influence tumorigenesis and progression. Key modifications, such N6‐methyladenosine (m 6 A) 5‐methylcytosine 5 C), implicated in various cellular processes. These regulated by proteins write, erase, read modulate stability, splicing, translation, degradation. Recent studies have highlighted their roles metabolic reprogramming, signaling pathways, cell cycle control, which essential for tumor proliferation survival. Despite these scientific advances, the precise mechanisms affect remain inadequately understood. This review comprehensively examines role play proliferation, metastasis, programmed death, including apoptosis, autophagy, ferroptosis. It explores effects on epithelial–mesenchymal transition (EMT) immune microenvironment, particularly metastasis. Furthermore, modifications’ potential therapies, conventional treatments, immunotherapy, targeted is discussed. By addressing aspects, this aims to bridge current research gaps underscore therapeutic of targeting improve treatment strategies patient outcomes.

Язык: Английский

Процитировано

1
ALKBH3‐Mediated M1A Demethylation of METTL3 Endows Pathological Fibrosis:Interplay Between M1A and M6A RNA Methylation DOI Creative Commons
Liying Tu, Shuchen Gu, Ruoqing Xu

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract Epigenetic modifications serve as crucial molecular switches for pathological fibrosis; howbeit the role of m 1 A in this condition remains enigmatic. Herein, it is found that ALKBH3 exerts a pro‐fibrotic effect skin fibrosis by reshaping N6‐methyladenosine (m 6 A) RNA modification pattern. First, exhibited specific upregulation within hypertrophic scars (HTS), accompanied N1‐methyladenosine hypomethylation. Moreover, multiomics analyses identified METTL3, critical writer enzyme involved modification, downstream candidate target ALKBH3. Therapeutically, ablation inhibited progression HTS both vitro and vivo, while exogenous replenishment METTL3 counteracted antifibrotic effect. Mechanistically, recognizes methylation sites prevents YTHDF2‐dependent mRNA decay transcript. Subsequently, stabilizes collagen type I alpha chain ( COL1A1 ) fibronectin1 FN1 mRNAs, two major components extracellular matrix, therefore eliciting transformation HTS. This observation bridges understanding link between methylation, fundamental modifications, underscoring participation “RNA crosstalk” events.

Язык: Английский

Процитировано

1
Nanoparticle Formulations for Intracellular Delivery in Colorectal Cancer Therapy DOI
Burcu Üner,

Erdogan Oguzhan Akyildiz,

Kübra Kolci

и другие.

AAPS PharmSciTech, Год журнала: 2025, Номер 26(3)

Опубликована: Март 7, 2025

Язык: Английский

Процитировано

0
The secret life of N1-methyladenosine: a review on its regulatory functions DOI
Nathalie Nguyen, Xi-Sheng Liu, Anindya Dutta

и другие.

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169099 - 169099

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
RNA modifications in the tumor microenvironment: insights into the cancer-immunity cycle and beyond DOI Creative Commons
Yuan Ding, Cuicui Liu, Ke‐Da Yu

и другие.

Experimental Hematology and Oncology, Год журнала: 2025, Номер 14(1)

Опубликована: Апрель 2, 2025

Язык: Английский

Процитировано

0
Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas DOI Creative Commons
Tim Hohmann, Urszula Grabiec,

Faramarz Dehghani

и другие.

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(4), С. 266 - 266

Опубликована: Апрель 9, 2025

Current research discusses the putative importance of RNA modification in tumor diseases. These modifications include predominantly pseudouridinylation, ortho-methylations on ribose residues, as well methylations organic bases. Such chemical directly influence fundamental properties such transcript stability, alternative splicing, and translation efficiency, all which are basic requirements for (tumor) cell proliferation, metabolism, migration, apoptosis resistance, etc. In this comparative study, two RNA-modifying factors, pseudouridine synthase 7 (PUS7, pseudouridinylation) WT1-associated protein (WTAP, m6A methylation), were identified using data from human renal carcinoma (RCC) tumors. PUS7 WTAP showed a statistically significant correlation with relevant proliferation prognosis markers CXCR4, TP53, PTEN, NRAS, immune checkpoints HLA-G LGALS9 associated effect overall survival. Furthermore, analyses also further target mRNAs biology WTAP. particular, components direct relevance mitosis, cycle, division, WNT pathway, identified.

Язык: Английский

Процитировано

0
Chemical and Enzyme‐Mediated Chemical Reactions for Studying Nucleic Acids and Their Modifications DOI

Tengwei Li,

Chongguang Cheng,

Jianzhao Liu

и другие.

ChemBioChem, Год журнала: 2024, Номер 25(15)

Опубликована: Май 14, 2024

Nucleic acids are genetic information-carrying molecules inside cells. Apart from basic nucleotide building blocks, there exist various naturally occurring chemical modifications on nucleobase and ribose moieties, which greatly increase the encoding complexity of nuclei acids, contribute to alteration nucleic acid structures, play versatile regulation roles in gene expression. To study functions certain biological contexts, robust tools specifically label identify these macromolecules their modifications, illuminate structures highly necessary. In this review, we summarize recent technique advances using enzyme-mediated reactions structures. By highlighting principles techniques, aim present a perspective advancement field as well offer insights into developing specific precise enzyme catalysis utilized for modifications.

Язык: Английский

Процитировано

2
Single-cell transcriptomics reveals writers of RNA modification-mediated immune microenvironment and cardiac resident Macro-MYL2 macrophages in heart failure DOI Creative Commons

Yao‐Lin Yang,

Xiaowei Li, Hai‐Bin Chen

и другие.

BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 16, 2024

Heart failure (HF), which is caused by cardiac overload and injury, linked to significant mortality. Writers of RNA modification (WRMs) play a crucial role in the regulation epigenetic processes involved immune response cardiovascular disease. However, potential roles these writers immunological milieu HF remain unknown. We comprehensively characterized expressions 28 WRMs using datasets GSE145154 GSE141910 map microenvironment patients. Based on expression WRMs, cells were scored. Single-cell transcriptomics analysis (GSE145154) revealed dysregulation as well differential from non-HF (NHF) samples. WRM-scored positively correlated with response, high WRM score group exhibited elevated cell infiltration. are differentiation T myeloid cells. scores subtypes significantly reduced compared NHF group. identified myogenesis-related resident macrophage population heart, Macro-MYL2, that was an increased cardiomyocyte structural genes (MYL2, TNNI3, TNNC1, TCAP, TNNT2) regulated TRMT10C. pattern, data (GSE141910) two distinct clusters samples, each functional enrichments characteristics. Our study demonstrated relationship between HF, novel population, myogenesis. These results provide perspective underlying mechanisms therapeutic targets for HF. Further experiments required validate Macro-MYL2 subtype milieu.

Язык: Английский

Процитировано

2
The landscape of <i>N</i><sup>1</sup>-methyladenosine (m<sup>1</sup>A) modification in mRNA of the decidua in severe preeclampsia DOI Creative Commons
Jing Tong, Hua Li, Liang Zhang

и другие.

Biomolecules and Biomedicine, Год журнала: 2024, Номер 24(6), С. 1827 - 1847

Опубликована: Июль 3, 2024

Recent discoveries in mRNA modification have highlighted N1-methyladenosine (m1A), but its role preeclampsia (PE) pathogenesis remains unclear. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and (RNA-seq) to identify m1A peaks the expression profile of decidua humans with early-onset PE (EPE), late-onset (LPE), normal pregnancy (NP). We assessed patterns preeclamptic using 10 modulators. Our bioinformatic analysis focused on differentially mRNAs (DMGs) expressed (DEGs) pairwise comparisons EPE vs. NP, LPE LPE, as well m1A-related DEGs. The identified 3110, 2801, 2818 DMGs, respectively. discerned three different from data. Further revealed that key PE-related DMGs DEGs predominantly influence signaling pathways critical for decidualization, including cAMP, MAPK, PI3K-Akt, Notch, TGF-β pathways. Additionally, these modifications impact related vascular smooth muscle contraction, estrogen signaling, relaxin contributing dysfunction. findings demonstrate exhibits unique gene profiles significantly alter essential both decidualization These differences provide valuable insights into molecular mechanisms influencing process function PE. regulators could potentially serve potent biomarkers or therapeutic targets PE, warranting further investigation.

Язык: Английский

Процитировано

1