Exploratory Blood Biomarker Patterns in a Mixed Dementia Cohort DOI Creative Commons
Haşim Gezegen, Merve Alaylıoğlu, Erdi Şahin

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Alzheimer’s disease (AD) diagnosis is challenging due to overlapping symptoms with other dementias. Current diagnostic methods are invasive and costly, highlighting the need for accessible biomarkers. This study investigates performance pathophysiological implications of a novel plasma biomarker panel in mixed dementia cohort, aiming enhance elucidate underlying pathogenic mechanisms. 120 biomarkers were analyzed using NULISA™ platform well-characterized mixt dementia. CSF measured via ELISA. Statistical analyses employed ANOVA, Kruskal-Wallis tests group comparisons. Spearman correlations assessed relationships between Diagnostic accuracy was evaluated regression models ROC curves. Feature importance selection performed random forest analysis. Protein interactions GO enrichment We 248 subjects (130 females, 118 males) 117 AD, 50 MCI, 39 FTD, 25 DLB, 17 Plasma pTau significantly elevated AD compared groups, DLB MCI. Aβ42 highest while NfL FTD. GFAP MCI levels showed negative correlation positive entire cohort. also highly correlated. These stronger amyloid-positive groups but weaker or absent group. pTau, GFAP, negatively correlated MMSE FTD DLB. pTau217 demonstrated best amyloid positivity (AUCs 0.9, 0.84, 0.79, 0.87, respectively). pTau181, pTau217, pTau231, total-tau had lower odds AD. AGRN, CXCL1, SCNB, TEK, UCHL1 higher SNAP25 ratio MAPT, PGF related progression. Random analysis incorporating all biomarkers, age, gender yielded an AUCs 0.85 0.84 0.75 Refining model by including identified as significant improved performance, resulting 0.88 0.87 0.81 demonstrates potential enhancing through targeted refinement.

Язык: Английский

Metabolomic and Proteomic Profiling of Serum-Derived Extracellular Vesicles from Early-Stage Amyotrophic Lateral Sclerosis Patients DOI
Yara Al Ojaimi, Nicolas Vallet, Audrey Dangoumau

и другие.

Journal of Molecular Neuroscience, Год журнала: 2025, Номер 75(1)

Опубликована: Фев. 15, 2025

Язык: Английский

Процитировано

1

Intercellular communication via exosomes: A new paradigm in the pathophysiology of neurodegenerative disorders DOI

Kiran S Satao,

Gaurav Doshi

Life Sciences, Год журнала: 2025, Номер 365, С. 123468 - 123468

Опубликована: Фев. 13, 2025

Язык: Английский

Процитировано

0

Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI Open Access

María Ljungström,

Elisa Oltra

Genes, Год журнала: 2025, Номер 16(3), С. 330 - 330

Опубликована: Март 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Язык: Английский

Процитировано

0

Brain Metabolite Profiles are Associated with Selective Neuronal Vulnerability and Underlying Mechanisms in Amyotrophic Lateral Sclerosis DOI
Enam Alhagh Charkhat Gorgich, Zahra Heidari, Hamidreza Mahmoudzadeh‐Sagheb

и другие.

ACS Chemical Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Март 29, 2025

Amyotrophic lateral sclerosis (ALS) is a lethal neurological syndrome accompanied by selective degeneration of somatic motor neurons and neurochemistry alterations. Nevertheless, eye movement's nuclei are relatively spared from ALS damage. This survey was to probe metabolite changes in the primary cortex (PMC) interstitial nucleus Cajal (INC) patients using proton magnetic resonance spectroscopy (1H-MRS). In this case-control study, 20 with healthy controls underwent 1.5 T MRI multivoxel 1H-MRS. 1H-MRS spectra determine profiles including tNAA, mIns, tCr, tCho, also tNAA/tCr, tNAA/tCho, mIns/tNAA ratios PMC INC were quantified via point resolved pulse (PRESS) sequence two groups. Further, associations between markers forced vital capacity (FVC), functional rating scale (ALSFRS-R), disease progression rate (ΔFS) investigated. PMC, tNAA tNAA/tCr significantly lower than controls, but mIns greater these (p < 0.05). INC, tCho concentrations, ratio increased 0.05) patients, while did not show significant discriminations groups > The tNAA/Cr associated ALSFRS-R = 0.001, r 0.71), FVC 0.03, 0.58), ΔFS 0.01, -0.33). 0.02, 0.41). concentrations 0.04, -0.54) -0.38) negatively positively correlated 0.33) 0.61), respectively. study suggests that patients' brains linked neuronal vulnerability underlying pathophysiology disease.

Язык: Английский

Процитировано

0

Current potential diagnostic biomarkers of amyotrophic lateral sclerosis DOI
Zheqi Xu, Renshi Xu

Reviews in the Neurosciences, Год журнала: 2024, Номер 35(8), С. 917 - 931

Опубликована: Июль 8, 2024

Amyotrophic lateral sclerosis (ALS) currently lacks the useful diagnostic biomarkers. The current diagnosis of ALS is mainly depended on clinical manifestations, which contributes to delay and be difficult make accurate at early stage ALS, hinders therapeutics. more pathogenesis are found last 30 years, including excitotoxicity, oxidative stress, mitochondrial dysfunction, neuroinflammation, altered energy metabolism, RNA misprocessing most recent neuroimaging findings. findings these bring new clues for searching biomarkers ALS. At present, a large number relevant studies about underway. related might lessen reliance manifestations. Among them, cortical signatures patients derived from both structural functional magnetic resonance imaging emerging proteomic neuronal loss glial activation in cerebrospinal fluid as well potential blood, serum, urine, saliva leading phase Here, we reviewed

Язык: Английский

Процитировано

1

Exploring the potential of MFG-E8 in neurodegenerative diseases DOI
Dan Li, Rongchun Wang, Weihong Lu

и другие.

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 15

Опубликована: Окт. 28, 2024

Milk fat globule-epidermal growth factor 8 (MFG-E8) is a multifunctional glycoprotein regulating intercellular interactions in various biological and pathological processes. This review summarizes the effects mechanisms of MFG-E8 neurodegenerative diseases (NDDs), emphasizing its roles inflammation, apoptosis, oxidative stress. In this review, will also explore potential as diagnostic biomarker therapeutic applications disorders. Recent studies have revealed intriguing characteristics using drug for treating brain While discovery, origin, expression, physiological functions organs tissues are well defined, role remains less understood. particularly true NDDs, indicating unmet medical needs. Elucidating could position treatment NDDs.

Язык: Английский

Процитировано

1

Cerebrospinal Fluid-Derived extracellular Vesicle-Inspired Multifunctional bone regeneration scaffold for cranial defect repair DOI Creative Commons
Jie He, Yifan Zhang, Xiaolan Sun

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158908 - 158908

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0

Evaluation of carotid Intima-Media Thickness (IMT) in amyotrophic lateral sclerosis disease using ultrasonography DOI

Maryam Rezaee Semnani,

Zahra Mirzaasgari, Armin Ariaei

и другие.

Journal of Clinical Neuroscience, Год журнала: 2024, Номер 124, С. 67 - 72

Опубликована: Апрель 23, 2024

Язык: Английский

Процитировано

0

Exploratory Blood Biomarker Patterns in a Mixed Dementia Cohort DOI Creative Commons
Haşim Gezegen, Merve Alaylıoğlu, Erdi Şahin

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Alzheimer’s disease (AD) diagnosis is challenging due to overlapping symptoms with other dementias. Current diagnostic methods are invasive and costly, highlighting the need for accessible biomarkers. This study investigates performance pathophysiological implications of a novel plasma biomarker panel in mixed dementia cohort, aiming enhance elucidate underlying pathogenic mechanisms. 120 biomarkers were analyzed using NULISA™ platform well-characterized mixt dementia. CSF measured via ELISA. Statistical analyses employed ANOVA, Kruskal-Wallis tests group comparisons. Spearman correlations assessed relationships between Diagnostic accuracy was evaluated regression models ROC curves. Feature importance selection performed random forest analysis. Protein interactions GO enrichment We 248 subjects (130 females, 118 males) 117 AD, 50 MCI, 39 FTD, 25 DLB, 17 Plasma pTau significantly elevated AD compared groups, DLB MCI. Aβ42 highest while NfL FTD. GFAP MCI levels showed negative correlation positive entire cohort. also highly correlated. These stronger amyloid-positive groups but weaker or absent group. pTau, GFAP, negatively correlated MMSE FTD DLB. pTau217 demonstrated best amyloid positivity (AUCs 0.9, 0.84, 0.79, 0.87, respectively). pTau181, pTau217, pTau231, total-tau had lower odds AD. AGRN, CXCL1, SCNB, TEK, UCHL1 higher SNAP25 ratio MAPT, PGF related progression. Random analysis incorporating all biomarkers, age, gender yielded an AUCs 0.85 0.84 0.75 Refining model by including identified as significant improved performance, resulting 0.88 0.87 0.81 demonstrates potential enhancing through targeted refinement.

Язык: Английский

Процитировано

0