Exploratory Blood Biomarker Patterns in a Mixed Dementia Cohort DOI Creative Commons
Haşim Gezegen, Merve Alaylıoğlu, Erdi Şahin

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Alzheimer’s disease (AD) diagnosis is challenging due to overlapping symptoms with other dementias. Current diagnostic methods are invasive and costly, highlighting the need for accessible biomarkers. This study investigates performance pathophysiological implications of a novel plasma biomarker panel in mixed dementia cohort, aiming enhance elucidate underlying pathogenic mechanisms. 120 biomarkers were analyzed using NULISA™ platform well-characterized mixt dementia. CSF measured via ELISA. Statistical analyses employed ANOVA, Kruskal-Wallis tests group comparisons. Spearman correlations assessed relationships between Diagnostic accuracy was evaluated regression models ROC curves. Feature importance selection performed random forest analysis. Protein interactions GO enrichment We 248 subjects (130 females, 118 males) 117 AD, 50 MCI, 39 FTD, 25 DLB, 17 Plasma pTau significantly elevated AD compared groups, DLB MCI. Aβ42 highest while NfL FTD. GFAP MCI levels showed negative correlation positive entire cohort. also highly correlated. These stronger amyloid-positive groups but weaker or absent group. pTau, GFAP, negatively correlated MMSE FTD DLB. pTau217 demonstrated best amyloid positivity (AUCs 0.9, 0.84, 0.79, 0.87, respectively). pTau181, pTau217, pTau231, total-tau had lower odds AD. AGRN, CXCL1, SCNB, TEK, UCHL1 higher SNAP25 ratio MAPT, PGF related progression. Random analysis incorporating all biomarkers, age, gender yielded an AUCs 0.85 0.84 0.75 Refining model by including identified as significant improved performance, resulting 0.88 0.87 0.81 demonstrates potential enhancing through targeted refinement.

Language: Английский

Metabolomic and Proteomic Profiling of Serum-Derived Extracellular Vesicles from Early-Stage Amyotrophic Lateral Sclerosis Patients DOI
Yara Al Ojaimi, Nicolas Vallet, Audrey Dangoumau

et al.

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)

Published: Feb. 15, 2025

Language: Английский

Citations

1
Intercellular communication via exosomes: A new paradigm in the pathophysiology of neurodegenerative disorders DOI

Kiran S Satao,

Gaurav Doshi

Life Sciences, Journal Year: 2025, Volume and Issue: 365, P. 123468 - 123468

Published: Feb. 13, 2025

Language: Английский

Citations

0
Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI Open Access

María Ljungström,

Elisa Oltra

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 330 - 330

Published: March 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Language: Английский

Citations

0
Brain Metabolite Profiles are Associated with Selective Neuronal Vulnerability and Underlying Mechanisms in Amyotrophic Lateral Sclerosis DOI
Enam Alhagh Charkhat Gorgich, Zahra Heidari, Hamidreza Mahmoudzadeh‐Sagheb

et al.

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

Amyotrophic lateral sclerosis (ALS) is a lethal neurological syndrome accompanied by selective degeneration of somatic motor neurons and neurochemistry alterations. Nevertheless, eye movement's nuclei are relatively spared from ALS damage. This survey was to probe metabolite changes in the primary cortex (PMC) interstitial nucleus Cajal (INC) patients using proton magnetic resonance spectroscopy (1H-MRS). In this case-control study, 20 with healthy controls underwent 1.5 T MRI multivoxel 1H-MRS. 1H-MRS spectra determine profiles including tNAA, mIns, tCr, tCho, also tNAA/tCr, tNAA/tCho, mIns/tNAA ratios PMC INC were quantified via point resolved pulse (PRESS) sequence two groups. Further, associations between markers forced vital capacity (FVC), functional rating scale (ALSFRS-R), disease progression rate (ΔFS) investigated. PMC, tNAA tNAA/tCr significantly lower than controls, but mIns greater these (p < 0.05). INC, tCho concentrations, ratio increased 0.05) patients, while did not show significant discriminations groups > The tNAA/Cr associated ALSFRS-R = 0.001, r 0.71), FVC 0.03, 0.58), ΔFS 0.01, -0.33). 0.02, 0.41). concentrations 0.04, -0.54) -0.38) negatively positively correlated 0.33) 0.61), respectively. study suggests that patients' brains linked neuronal vulnerability underlying pathophysiology disease.

Language: Английский

Citations

0
Current potential diagnostic biomarkers of amyotrophic lateral sclerosis DOI
Zheqi Xu, Renshi Xu

Reviews in the Neurosciences, Journal Year: 2024, Volume and Issue: 35(8), P. 917 - 931

Published: July 8, 2024

Amyotrophic lateral sclerosis (ALS) currently lacks the useful diagnostic biomarkers. The current diagnosis of ALS is mainly depended on clinical manifestations, which contributes to delay and be difficult make accurate at early stage ALS, hinders therapeutics. more pathogenesis are found last 30 years, including excitotoxicity, oxidative stress, mitochondrial dysfunction, neuroinflammation, altered energy metabolism, RNA misprocessing most recent neuroimaging findings. findings these bring new clues for searching biomarkers ALS. At present, a large number relevant studies about underway. related might lessen reliance manifestations. Among them, cortical signatures patients derived from both structural functional magnetic resonance imaging emerging proteomic neuronal loss glial activation in cerebrospinal fluid as well potential blood, serum, urine, saliva leading phase Here, we reviewed

Language: Английский

Citations

1
Exploring the potential of MFG-E8 in neurodegenerative diseases DOI
Dan Li, Rongchun Wang, Weihong Lu

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Oct. 28, 2024

Milk fat globule-epidermal growth factor 8 (MFG-E8) is a multifunctional glycoprotein regulating intercellular interactions in various biological and pathological processes. This review summarizes the effects mechanisms of MFG-E8 neurodegenerative diseases (NDDs), emphasizing its roles inflammation, apoptosis, oxidative stress. In this review, will also explore potential as diagnostic biomarker therapeutic applications disorders. Recent studies have revealed intriguing characteristics using drug for treating brain While discovery, origin, expression, physiological functions organs tissues are well defined, role remains less understood. particularly true NDDs, indicating unmet medical needs. Elucidating could position treatment NDDs.

Language: Английский

Citations

1
Cerebrospinal Fluid-Derived extracellular Vesicle-Inspired Multifunctional bone regeneration scaffold for cranial defect repair DOI Creative Commons
Jie He, Yifan Zhang, Xiaolan Sun

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158908 - 158908

Published: Dec. 1, 2024

Language: Английский

Citations

0
Evaluation of carotid Intima-Media Thickness (IMT) in amyotrophic lateral sclerosis disease using ultrasonography DOI

Maryam Rezaee Semnani,

Zahra Mirzaasgari, Armin Ariaei

et al.

Journal of Clinical Neuroscience, Journal Year: 2024, Volume and Issue: 124, P. 67 - 72

Published: April 23, 2024

Language: Английский

Citations

0
Exploratory Blood Biomarker Patterns in a Mixed Dementia Cohort DOI Creative Commons
Haşim Gezegen, Merve Alaylıoğlu, Erdi Şahin

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Alzheimer’s disease (AD) diagnosis is challenging due to overlapping symptoms with other dementias. Current diagnostic methods are invasive and costly, highlighting the need for accessible biomarkers. This study investigates performance pathophysiological implications of a novel plasma biomarker panel in mixed dementia cohort, aiming enhance elucidate underlying pathogenic mechanisms. 120 biomarkers were analyzed using NULISA™ platform well-characterized mixt dementia. CSF measured via ELISA. Statistical analyses employed ANOVA, Kruskal-Wallis tests group comparisons. Spearman correlations assessed relationships between Diagnostic accuracy was evaluated regression models ROC curves. Feature importance selection performed random forest analysis. Protein interactions GO enrichment We 248 subjects (130 females, 118 males) 117 AD, 50 MCI, 39 FTD, 25 DLB, 17 Plasma pTau significantly elevated AD compared groups, DLB MCI. Aβ42 highest while NfL FTD. GFAP MCI levels showed negative correlation positive entire cohort. also highly correlated. These stronger amyloid-positive groups but weaker or absent group. pTau, GFAP, negatively correlated MMSE FTD DLB. pTau217 demonstrated best amyloid positivity (AUCs 0.9, 0.84, 0.79, 0.87, respectively). pTau181, pTau217, pTau231, total-tau had lower odds AD. AGRN, CXCL1, SCNB, TEK, UCHL1 higher SNAP25 ratio MAPT, PGF related progression. Random analysis incorporating all biomarkers, age, gender yielded an AUCs 0.85 0.84 0.75 Refining model by including identified as significant improved performance, resulting 0.88 0.87 0.81 demonstrates potential enhancing through targeted refinement.

Language: Английский

Citations

0