Association between gut microbiota and spinal stenosis: a two-sample mendelian randomization study

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Апрель 19, 2024

Considerable evidence has unveiled a potential correlation between gut microbiota and spinal degenerative diseases. However, only limited studies have reported the direct association stenosis. Hence, in this study, we aimed to clarify relationship using two-sample mendelian randomization (MR) approach.

Язык: Английский

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Gut microbiome dysbiosis is associated with lumbar degenerative spondylolisthesis in symptomatic patients

JOR Spine, Год журнала: 2024, Номер 7(4)

Опубликована: Окт. 10, 2024

Abstract Background Lumbar degenerative spondylolisthesis (LDS), characterized as degeneration of the intervertebral disc and structural changes facet joints, is a condition with varying degrees instability that may lead to pain, canal stenosis, subsequent surgical intervention. However, etiology LDS remains inconclusive. Gut microbiome dysbiosis stimulate systemic inflammation in various disorders. role such upon spine health under‐studied. The current study assessed association gut symptomatic patients or without LDS. Methods A cross‐sectional analysis within framework prospective was performed. DNA extracted from fecal samples collected adult ( n = 21) 12). Alpha beta diversity differences microbial community between groups. Taxon‐by‐taxon identified features differential relative abundance Subject demographics imaging parameters were also assessed. Results There no significant group age, sex, race, body mass index, smoking/alcohol history, pain profiles, spinopelvic alignment, Modic p >0.05). subjects had significantly higher severity 0.018) alpha levels compared non‐LDS 0.002–0.003). Significant structure observed groups 0.046). Subjects exhibited distinct at phylum level, Firmicutes Bacteroidota ratio 0.003). Differential six taxa two groups, demonstrating an increase putative pro‐inflammatory bacteria Dialister, CAG‐352 ) decrease anti‐inflammatory Slackia , Escherichia‐Shigella ). Conclusion This first report patients, noting bacterial taxa. work provides foundation for future studies addressing disease.

Язык: Английский

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Genetic Causal Association Between Skin Microbiota and Biological Aging: Evidence From a Mendelian Randomization Analysis

Journal of Cosmetic Dermatology, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 1, 2025

ABSTRACT Background The skin microbiota, a complex community of microorganisms residing on the skin, plays crucial role in maintaining health and overall homeostasis. Recent research has suggested that alterations composition function microbiota may influence aging process. However, causal relationships between specific biological remain unclear. Mendelian randomization (MR) analysis provides powerful tool to explore these links by utilizing genetic variants as instrumental variables, thereby minimizing confounding factors reverse causality often complicate observational studies. Methods We utilized two‐sample MR approach with population‐based cross‐sectional data from two German cohorts, KORA FF4 ( n = 324) PopGen 273). In total, GWAS summary 1656 samples datasets accelerated age were analyzed investigate relationship aging. primary was performed using inverse variance weighted (IVW) method random effects further supported MR‐Egger regression, Cochran's Q test, range sensitivity analyses. Results revealed for acceleration (BioageAccel), IVW identified protective certain including Alphaproteobacteria_Dry p 0.046), Asv033_sebaceous 0.043), Burkholderiales_Moist 0.008), Proteobacteria_Moist 0.042). Similar observed 0.045) 0.012) median analysis. contrast, Paracoccus_Moist 0.013) Proteobacteria_Sebaceous 0.005) associated When PhenoAge outcome, linked like Asv005_Dry 0.026), ASV039_Dry 0.003), Betaproteobacteria_Sebaceous 0.038), Chryseobacterium_Moist findings also ASV011_Dry 0.021), ASV023_Dry 0.040), Bacteroidales_Dry 0.022), Enhydrobacter_Moist 0.002), Rothia_Moist 0.038). Conclusions This study reveals potential confirm findings, randomized controlled trials (RCTs) are necessary.

Язык: Английский

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Prevention of surgical site infection: need to focus on the enemy within

Journal of Joint Surgery and Research, Год журнала: 2025, Номер 3(1), С. 73 - 77

Опубликована: Фев. 27, 2025

Язык: Английский

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Causal Correlations Between Plasma Metabolites, Inflammatory Proteins, and Chronic Obstructive Pulmonary Disease: A Mendelian Randomization and Bioinformatics-Based Investigation

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 4057 - 4073

Опубликована: Март 1, 2025

An increasing number of studies have demonstrated a strong correlation between metabolism, inflammation, and chronic obstructive pulmonary disease (COPD). However, it remains unclear if there is causal relationship these factors. This study employed the Mendelian randomization (MR) approach to investigate associations factors explore mediating roles key inflammatory proteins. MR was used assess plasma metabolites, proteins, COPD. Sensitivity analyses were performed verify robustness findings. Mediation analysis conducted proteins in metabolism-COPD pathway. We constructed protein-protein interaction (PPI) network explored potential mechanism through gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment. Single-cell sequencing transcriptome datasets for auxiliary validation. Finally, experimental validation using human lung tissue. identified 63 10 metabolite ratios, 48 that associated with COPD, all which exhibited relationships. Furthermore, three as mediators metabolite-to-COPD PPI network, GO KEGG enrichment revealed biological pathways they involved. Validation expression intermediary tissue NRXN3 expressed endothelial cells exerted protective effect against COPD development. The among These findings offer novel insights into metabolism-inflammation-COPD mechanisms, suggesting interventions targeting metabolic processes may represent promising strategy preventing onset or progression

Язык: Английский

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A Mendelian randomization study to reveal gut-disc axis: causal associations between gut microbiota with intervertebral disc diseases

European Spine Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Язык: Английский

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Guiqi Huoxue Capsule Alleviates Cervical Spondylosis in Rats: Insights from 16S rRNA Sequencing, Lipidomics, and Network Pharmacology

Journal of Ethnopharmacology, Год журнала: 2025, Номер 347, С. 119752 - 119752

Опубликована: Апрель 7, 2025

Язык: Английский

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Exploring the role of gut microbiota in intervertebral disc degeneration: insights from bidirectional Mendelian randomization analysis

European Spine Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Although previous studies have indicated a potential association between the gut microbiota and intervertebral disc degeneration (IVDD), precise nature of this relationship remains unclear. The objective study is to further explore causal IVDD using bidirectional Mendelian randomization approach, with aim identifying microbial characteristics associated IVDD. Using data from genome-wide (GWAS) involving 412 species 227,388 controls 29,508 cases Inverse variance weighted (IVW) was used as primary (MR) analysis, complemented by median, MR-Egger regression, mode simple methods. Extensive sensitivity analyses were performed confirm robustness results assess heterogeneity horizontal pleiotropy. This revealed positive genetic predisposition 6 types through IVW method, indicating that increased levels these may lead higher risk Conversely, found negative effects on IVDD, suggesting protective effect against Reverse MR analysis such possibilities 1 5 relationships microbiota. Cochran's Q test, MR-PRESSO all yielded p-values greater than 0.05, there no significant or pleiotropy in We approach identify various Our findings lay foundation for exploration pathogenesis treatment strategies provide new research biomarkers IVDD-related metabolic

Язык: Английский

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Roles of organokines in intervertebral disc homeostasis and degeneration

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Март 12, 2024

The intervertebral disc is not isolated from other tissues. Recently, abundant research has linked homeostasis and degeneration to various systemic diseases, including obesity, metabolic syndrome, diabetes. Organokines are a group of diverse factors named for the tissue origin, adipokines, osteokines, myokines, cardiokines, gastrointestinal hormones, hepatokines. Through endocrine, paracrine, autocrine mechanisms, organokines modulate energy homeostasis, oxidative stress, balance in tissues mediate cross-organ communication. These molecules involved regulation cellular behavior, inflammation, matrix metabolism under physiological pathological conditions. In this review, we aimed summarize impact on underlying signaling mechanism. We focused regulatory mechanisms provide basis development early diagnostic therapeutic strategies degeneration.

Язык: Английский

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Gut microbiota and radiculopathy: a bidirectional two-sample Mendelian randomization study

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 18, 2024

Abstract Background Previous studies have suggested a potential link between the gut microbiome and radiculopathy, but causal relationship remains unclear. Therefore, aim of this study was to determine effect on radiculopathy using Mendelian randomization (MR) approach single nucleotide polymorphisms (SNPs) associated with as instrumental variables Methods Summary data from genome-wide association microbiota (the MiBioGen) FinnGen biobank) were acquired. The inverse variance weighting (IVW) chosen main MR Analysis method. weighted median, MR-Egger regression, model, simple model provided additional supplements. pleiotropy residual sum outlier (MR-PRESSO) regression performed evaluate horizontal eliminate single-nucleotide (SNPs). Cochran’s Q-test applied for heterogeneity detection. Results We identified eight microbial taxa that causally (P<0.05). Cochrane Q test produced results did not indicate (P>0.05), indicating lack statistical significance. Furthermore, both intercept MR-PRESSO global indicated our findings influenced by further supporting reliability results. In reverse analysis, no evidence found suggest has an impact microbiota. Conclusion four protective against may elevate risk condition. Our confirmed thereby providing theoretical foundation development targeted prevention strategies. Keywords

Язык: Английский

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