Causal relationship between gut microbiota and lung squamous cell carcinoma: a bidirectional two-sample Mendelian randomization study DOI
Weijian Song, Jianwei Shi,

Minjun Du

и другие.

Postgraduate Medical Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

Abstract Purpose This study aims to explore the potential causal relationship between gut microbiota and lung squamous cell carcinoma (LUSC). Methods A bidirectional two-sample Mendelian randomization analysis was conducted using genome-wide association (GWAS) data from LUSC. Gut served as exposure factor, with instrumental variables selected a GWAS involving 18 340 participants. LUSC were drawn European cohort including 29 266 cases 56 450 controls. Inverse-variance weighted (IVW) method used primary method, Benjamini-Hochberg applied adjust for multiple comparisons. An independent dataset (ieu-a-967, containing 3275 15 038 controls) replication ensure robustness. Results IVW found that Butyricicoccus (OR = 0.79, 95% CI: 0.63–0.99, P .042) Coprobacter 0.85, 0.74–0.97, .018) significantly protective against In contrast, Victivallis 1.11, 1.00–1.23, .045) Ruminococcus 1.28, 1.03–1.60, .028) increased risk. Replication in confirmed significant associations Coprobacter. No reverse causality or pleiotropy detected. Conclusion provides evidence of specific risk, highlighting new microbial targets prevention treatment strategies cancer. Key messages What is already known on this topic? Previous studies have suggested links composition development various cancers, However, exact (LUSC) has remained unclear. Traditional observational struggled determine direction due confounding factors, making further investigation necessary through more robust methods such (MR). adds? MR novel genetic indicating certain microbiotas are causally associated Specifically, appears reduce risk LUSC, while increases These findings highlight role gut-lung axis open up avenues exploring modulators cancer How might affect research, practice, policy? The implications may influence future research into by targeting microbiota. Additionally, it could inform clinical practices aimed at modulating lower potentially influencing dietary probiotic interventions susceptibility. Furthermore, these results shape public health policies focus avenue management.

Язык: Английский

Genetically predicted plasma metabolites mediate the causal relationship between gut microbiota and osteosarcoma DOI Creative Commons

Xiuyu Qin,

Zhuming Fan,

Shigang Qiao

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 1, 2025

Previous research has demonstrated a close connection between the development of osteosarcoma (OS) and variations in abundance specific gut microbiota (GM). Generally speaking, GM play role human health mostly through metabolites. However, causal relationship GM, plasma metabolites, OS remains unclear. Hence, our study, we aim to clarify this OS, by employing Mendelian randomization (MR) approach. In pooled data were derived from public genome-wide association study (GWAS) (GCST90032172 GCST90032644), metabolites (GCST90199621 GCST90204603) (finngen_R10_C3_OSTEOSARCOMA_EXALLC). The two-sample two-step MR methods used for current analysis: (1) genetic causality on OS; (2) mediation effects For evaluating previously described relationship, inverse variance weighted (IVW) method was primarily used, with complementary approaches including median, MR-Egger, mode, simple mode. Moreover, MR-Egger intercept test mendelian pleiotropy residual sum outlier (MR-PRESSO) employed assess horizontal multiplicity. reliability is verified "leave-one-out" sensitivity analysis Cochran's Q heterogeneity. STROBE-MR checklist reporting studies study. First, according IVW results, 13 types specifically, identified have potential OS. After FDR correction, Phocea defined as strain clear (FDR-adjusted p < 0.05). Second, total 48 30 currently known 7 not yet studied, 11 metabolite ratios. Finally, further explored whether mediate And result, two Eugenol sulfate levels (mediated proportion: 7.74% (14.2%, 1.3%)) N-acetylphenylalanine 3.52% (6.18%, 0.867%)), that may link identified. All above results subjected analysis. revealed Importantly, also mediating modulating Of course, needs be conducted verify findings.

Язык: Английский

Процитировано

0
The causal association between type 2 diabetes and spinal stenosis: A Mendelian randomization analysis DOI Creative Commons

Zhaopeng Fan,

Bohong Chen,

Le Ding

и другие.

Medicine, Год журнала: 2024, Номер 103(39), С. e39894 - e39894

Опубликована: Сен. 27, 2024

Spinal stenosis is a prevalent degenerative spinal disease and one of the main causes pain dysfunction in older adults. Substantial evidence indicates potentially relevant association between type 2 diabetes mellitus (T2DM) stenosis. However, causality these disorders remains unclear. Therefore, we intended to elucidate this relationship using Mendelian Randomization (MR) analysis study. Based on genome-wide study (GWAS) data T2DM stenosis, performed bidirectional 2-sample MR evaluate We assessed heterogeneity Cochran’s Q statistic horizontal pleiotropy MR-Egger-intercept. “Leave-one-out” was determine reliability causal relationships. In addition, conducted multivariate clarify direct influence after accounting for effect body mass index (BMI) Our results indicated that Individuals with had heightened risk (odds ratio [OR]: 1.050; 95% CI: 1.004–1.098, P = .031). Moreover, no reverse existed The sensitivity suggest steady robust. Multivariate demonstrated not related BMI (OR, 1.047; 1.003–1.093; .032). analyses possible positive unrelated BMI.

Язык: Английский

Процитировано

2
Exploring the Ocular Surface Microbiome and Tear Proteome in Glaucoma DOI Open Access

Livia A. Spörri,

Anne‐Christine Uldry, Marco Kreuzer

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6257 - 6257

Опубликована: Июнь 6, 2024

Although glaucoma is a leading cause of irreversible blindness worldwide, its pathogenesis incompletely understood, and intraocular pressure (IOP) the only modifiable risk factor to target disease. Several associations between gut microbiome glaucoma, including IOP, have been suggested. There growing evidence that interactions microbes on ocular surface, termed surface (OSM), tear proteins, collectively called proteome, may also play role in diseases such as glaucoma. This study aimed find characteristic features OSM proteins patients with The whole-metagenome shotgun sequencing 32 conjunctival swabs identified Actinobacteria, Firmicutes, Proteobacteria dominant phyla cohort. species Corynebacterium mastitidis was found healthy controls, their microbiomes be enriched genes phospholipase pathway compared patients. Despite these minor differences OSM, showed an enrichment many associated immune system controls. In contrast this emphasizes potential involvement immunological processes These findings contribute design new therapeutic approaches targeting other diseases.

Язык: Английский

Процитировано

1
Causal relationship between gut microbiota and lung squamous cell carcinoma: a bidirectional two-sample Mendelian randomization study DOI
Weijian Song, Jianwei Shi,

Minjun Du

и другие.

Postgraduate Medical Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

Abstract Purpose This study aims to explore the potential causal relationship between gut microbiota and lung squamous cell carcinoma (LUSC). Methods A bidirectional two-sample Mendelian randomization analysis was conducted using genome-wide association (GWAS) data from LUSC. Gut served as exposure factor, with instrumental variables selected a GWAS involving 18 340 participants. LUSC were drawn European cohort including 29 266 cases 56 450 controls. Inverse-variance weighted (IVW) method used primary method, Benjamini-Hochberg applied adjust for multiple comparisons. An independent dataset (ieu-a-967, containing 3275 15 038 controls) replication ensure robustness. Results IVW found that Butyricicoccus (OR = 0.79, 95% CI: 0.63–0.99, P .042) Coprobacter 0.85, 0.74–0.97, .018) significantly protective against In contrast, Victivallis 1.11, 1.00–1.23, .045) Ruminococcus 1.28, 1.03–1.60, .028) increased risk. Replication in confirmed significant associations Coprobacter. No reverse causality or pleiotropy detected. Conclusion provides evidence of specific risk, highlighting new microbial targets prevention treatment strategies cancer. Key messages What is already known on this topic? Previous studies have suggested links composition development various cancers, However, exact (LUSC) has remained unclear. Traditional observational struggled determine direction due confounding factors, making further investigation necessary through more robust methods such (MR). adds? MR novel genetic indicating certain microbiotas are causally associated Specifically, appears reduce risk LUSC, while increases These findings highlight role gut-lung axis open up avenues exploring modulators cancer How might affect research, practice, policy? The implications may influence future research into by targeting microbiota. Additionally, it could inform clinical practices aimed at modulating lower potentially influencing dietary probiotic interventions susceptibility. Furthermore, these results shape public health policies focus avenue management.

Язык: Английский

Процитировано

0