Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 6, 2023
Abstract
Background
Uveal
melanoma
is
the
most
common
non-cutaneous
and
an
intraocular
malignancy
affecting
nearly
7,000
individuals
per
year
worldwide.
Of
these,
approximately
50%
will
progress
to
metastatic
disease
for
which
there
are
currently
no
effective
therapies.
Despite
advances
in
molecular
profiling
stratification
of
uveal
tumors,
little
known
regarding
their
underlying
biology
metastasis.
Our
group
has
identified
a
disseminated
neoplastic
cell
population
characterized
by
co-expression
immune
proteins,
circulating
hybrid
cells
(hybrids),
patients
with
melanoma.
Compared
tumor
cells,
lack
expression
hybrids
detected
at
increased
prevalence
peripheral
blood
can
be
used
as
non-invasive
biomarker
predict
progression.
Methods
To
ascertain
mechanisms
enhanced
dissemination
we
within
primary
tumors
using
single
RNA
sequencing
evaluated
gene
predicted
ligand-receptor
interactions
relation
other
tumor.
We
then
verified
upregulated
pathways
patient-matched
cyclic
immunofluorescence
quantified
protein
relative
non-hybrid
cells.
Results
Among
top
genes
were
those
involved
motility
cytoskeletal
rearrangement,
evasion,
altered
cellular
metabolism.
In
blood,
examining
concordant
each
pathway
category:
TMSB10
(cell
motility),
CD74
(immune
evasion)
GPX1
(metabolism).
Both
expressed
on
significantly
higher
numbers
compared
more
than
tumor-resident
hybrids.
Lastly,
that
express
signaling
implicated
promoting
metastasis
including
GAS6-AXL,
CXCL12-CXCR4,
LGALS9-P4HB
IGF1-IGFR1.
Conclusion
These
findings
highlight
importance
TMSB10,
successful
survival
circulation.
results
contribute
understanding
progression
between
microenvironment
may
promote
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Авг. 2, 2024
Abstract
Metastasis
remains
a
pivotal
characteristic
of
cancer
and
is
the
primary
contributor
to
cancer-associated
mortality.
Despite
its
significance,
mechanisms
governing
metastasis
are
not
fully
elucidated.
Contemporary
findings
in
domain
biology
have
shed
light
on
molecular
aspects
this
intricate
process.
Tumor
cells
undergoing
invasion
engage
with
other
cellular
entities
proteins
en
route
their
destination.
Insights
into
these
engagements
enhanced
our
comprehension
principles
directing
movement
adaptability
metastatic
cells.
The
tumor
microenvironment
plays
role
facilitating
proliferation
by
enabling
navigate
through
stromal
barriers.
Such
attributes
influenced
genetic
epigenetic
changes
occurring
surrounding
milieu.
A
profound
understanding
process’s
biological
indispensable
for
devising
efficacious
therapeutic
strategies.
This
review
delves
recent
developments
concerning
metastasis-associated
genes,
important
signaling
pathways,
microenvironment,
metabolic
processes,
peripheral
immunity,
mechanical
forces
metastasis.
In
addition,
we
combine
advances
particular
emphasis
prospect
developing
effective
interventions
including
most
popular
immunotherapies
nanotechnology
combat
We
also
identified
limitations
current
research
metastasis,
encompassing
drug
resistance,
restricted
animal
models,
inadequate
biomarkers
early
detection
methods,
as
well
heterogeneity
among
others.
It
anticipated
that
comprehensive
will
significantly
contribute
advancement
research.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 23, 2024
Bone
is
a
common
organ
for
solid
tumor
metastasis.
Malignant
bone
becomes
insensitive
to
systemic
therapy
after
colonization,
followed
by
poor
prognosis
and
high
relapse
rate.
Immune
cells
in
situ
constitute
unique
immune
microenvironment,
which
plays
crucial
role
the
context
of
This
review
firstly
focuses
on
lymphatic
metastatic
cancer,
including
their
function
dissemination,
invasion,
growth
possible
cytotoxicity-induced
eradication.
Subsequently,
we
examine
myeloid
cells,
namely
macrophages,
myeloid-derived
suppressor
dendritic
megakaryocytes,
evaluating
interaction
with
cytotoxic
T
lymphocytes
contribution
As
important
components
skeletal
tissue,
osteoclasts
osteoblasts
derived
from
marrow
stromal
engaging
‘vicious
cycle’
accelerate
osteolytic
We
also
explain
concept
dormancy
investigate
underlying
microenvironment
it.
Additionally,
thorough
emerging
treatments
malignancy
clinical
research,
especially
immunotherapy,
presented,
indicating
current
challenges
opportunities
research
development
metastasis
therapies.
Genes,
Год журнала:
2024,
Номер
15(4), С. 435 - 435
Опубликована: Март 29, 2024
Salmonella
typhimurium
(S.
typhimurium),
a
prevalent
cause
of
foodborne
infection,
induces
significant
changes
in
the
host
transcriptome
and
metabolome.
The
lack
therapeutics
with
minimal
or
no
side
effects
prompts
scientific
community
to
explore
alternative
therapies.
This
study
investigates
therapeutic
potential
probiotic
mixture
comprising
Lactobacillus
acidophilus
(L.
1.3251)
plantarum
9513)
against
S.
typhimurium,
utilizing
metabolomic
analyses,
novel
approach
that
has
not
been
previously
documented.
Twenty-four
SPF-BALB/c
mice
were
divided
into
four
groups:
control
negative
group
(CNG);
positive
(CPG);
probiotic-supplemented
non-challenged
(LAPG);
Salmonella-challenged
(LAPST).
An
RNA-sequencing
analysis
small
intestinal
(ileum)
tissue
revealed
2907
upregulated
394
downregulated
DEGs
LAPST
vs.
CPG
group.
A
functional
highlighted
their
significantly
altered
gene
ontology
(GO)
terms
related
metabolism,
gut
integrity,
cellular
development,
immunity
(p
≤
0.05).
KEGG
showed
differentially
expressed
genes
(DEGs)
primarily
involved
pathways
immunity,
such
as
MAPK,
PI3K-Akt,
AMPK,
tryptophan
glycine,
serine,
threonine
ECM–receptor
interaction,
others.
Additionally,
fecal
metabolic
identified
1215
305
metabolites
group,
implying
involvement
including
bile
secretion,
propanoate
arginine
proline
amino
acid
biosynthesis,
protein
digestion
absorption,
which
are
vital
for
maintaining
barrier
metabolism.
In
conclusion,
these
findings
suggest
administration
improves
maintains
homeostasis
enhances
metabolism
infection.
Biomarker Research,
Год журнала:
2024,
Номер
12(1)
Опубликована: Июль 20, 2024
Abstract
Background
Uveal
melanoma
is
the
most
common
non-cutaneous
and
an
intraocular
malignancy
affecting
nearly
7,000
individuals
per
year
worldwide.
Of
these,
approximately
50%
will
progress
to
metastatic
disease
for
which
there
are
currently
no
effective
curative
therapies.
Despite
advances
in
molecular
profiling
stratification
of
uveal
tumors,
little
known
regarding
their
underlying
biology
metastasis.
Our
group
has
identified
a
disseminated
neoplastic
cell
population
characterized
by
co-expression
immune
proteins,
circulating
hybrid
cells
(hybrids),
patients
with
melanoma.
Compared
tumor
cells,
lack
expression
hybrids
detected
at
increased
prevalence
peripheral
blood
can
be
used
as
non-invasive
biomarker
predict
progression.
Methods
To
ascertain
mechanisms
enhanced
dissemination
we
within
primary
tumors
using
single
RNA
sequencing
(
n
=
8)
evaluated
gene
predicted
ligand-receptor
interactions
relation
other
tumor.
We
then
verified
upregulated
pathways
patient-matched
4)
cyclic
immunofluorescence
quantified
protein
relative
non-hybrid
cells.
Results
Among
top
genes
were
those
involved
motility
cytoskeletal
rearrangement,
evasion,
altered
cellular
metabolism.
In
blood,
examining
concordant
each
pathway
category:
TMSB10
(cell
motility),
CD74
(immune
evasion)
GPX1
(metabolism).
Both
expressed
on
significantly
higher
numbers
compared
more
than
tumor-resident
hybrids.
Lastly,
that
express
signaling
implicated
promoting
metastasis
including
GAS6-AXL,
CXCL12-CXCR4,
LGALS9-P4HB
IGF1-IGFR1.
Conclusion
These
findings
highlight
importance
TMSB10,
successful
survival
circulation.
results
contribute
understanding
progression
between
microenvironment
may
promote
Oncology Letters,
Год журнала:
2025,
Номер
29(5), С. 1 - 12
Опубликована: Март 6, 2025
The
majority
of
cancer‑related
deaths
result
from
tumor
metastasis,
with
bone
metastasis
occurring
in
almost
all
types
malignant
tumors.
Understanding
the
mechanism
by
which
tumors
metastasize
to
is
critical
for
identification
novel
therapeutic
targets.
A
large
amount
research
has
been
carried
out
using
animal
models,
and
these
models
have
crucial
advancing
fundamental
understanding
cancer.
However,
current
are
limited;
although
they
can
mimic
specific
stages
metastatic
process,
not
able
replicate
entire
process
tumorigenesis
metastasis.
present
review
describes
molecular
changes
that
occur
intraosseous
microenvironment
metastases,
including
osteolytic
osteoblastic
types,
summarizes
advancements
Biomarker Research,
Год журнала:
2025,
Номер
13(1)
Опубликована: Март 5, 2025
The
tumour
microenvironment
is
the
"hotbed"
of
cells,
providing
abundant
extracellular
support
for
growth
and
metastasis.
However,
not
static
constantly
remodelled
by
a
variety
cellular
components,
including
through
mechanical,
biological
chemical
means
to
promote
Focal
adhesion
plays
an
important
role
in
cell-extracellular
matrix
adhesion.
An
in-depth
exploration
focal
metastasis,
especially
their
contribution
at
biomechanical
level,
direction
current
research.
In
this
review,
we
first
summarize
assembly
adhesions
explore
kinetics
cells.
Then,
describe
detail
various
stages
its
key
functions
cell
migration,
invasion,
remodelling.
Finally,
anti-tumour
strategies
targeting
progress
development
some
inhibitors
against
proteins.
paper,
time
that
play
positive
feedback
pro-tumour
metastatic
remodelling
summarizing
five
processes
multidimensional
way.
It
beneficial
researchers
have
deeper
understanding
behaviour
metastasis
potential
as
therapeutic
target,
new
ideas
prevention
treatment
metastases.
