Evaluation and preservation of fertility in patients with hematologic malignancies

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217569 - 217569

Опубликована: Фев. 1, 2025

Язык: Английский

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Mitochondrial Oxidative Phosphorylation Alterations in Placental Tissues from Early- and Late-Onset Preeclampsia

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 3951 - 3951

Опубликована: Апрель 22, 2025

Preeclampsia (PE), a pregnancy complication characterized by high blood pressure and organ damage, has been suggested to be associated with mitochondrial dysfunction, although evidence remains limited. This study aimed investigate the activity of oxidative phosphorylation (OXPHOS) enzymes expression related proteins in placental tissues from women diagnosed early-onset preeclampsia (eoPE, <34 weeks gestation), late-onset (loPE, ≥34 normotensive controls. Placental samples were analyzed using immunohistochemistry, western blotting, enzymatic assays assess OXPHOS complexes. Complex I was increased 80% eoPE 56% loPE, positive correlations between normalized complex expression, gestational age at delivery (r = 0.85, p 0.01), birth weight 0.88, 0.004) loPE. Relative II loPE showed duration 0.76, 0.03) 0.77, 0.03), while controls, correlated 0.64, 0.03). Additionally, IV enzyme negatively maternal −0.69, The observed highlight metabolism as promising biomarker for predicting disease progression guiding therapeutic interventions preeclampsia. Unraveling its precise role PE pathogenesis is critical advancing diagnostic precision improving maternal-fetal outcomes.

Язык: Английский

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Maternal age is associated with apoptotic gene abundance patterns in blastocoel fluid-conditioned media from euploid embryos: a pilot study

Journal of Assisted Reproduction and Genetics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract Purpose This retrospective study measured global gene abundance using RNASeq of blastocoel fluid-conditioned media from euploid ICSI-generated embryos to identify genes and signaling pathways associated with maternal age. Methods Blastocoel was obtained following trophectoderm biopsy day-5 blastocysts. Media for were 24 blastocysts (9 patients aged 35 or older). Transcriptome analysis identified differentially expressed when comparing advanced age those younger than 35. Further on the conducted another group samples RT-qPCR. Results Twenty-five protein encoding in under Genes proteins SHARPIN BCL2L12 showed a statistically significant increase ( p < 0.05) Abundance RT-qPCR additional revealed elevated successful implantation alongside decrease CASP8 abundance. pattern opposite Conclusions uncovered differential apoptotic by assessing These unique patterns may provide insight into regulation apoptosis women age, how this pathway impact outcomes.

Язык: Английский

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The Importance of Mitochondrial Processes in the Maturation and Acquisition of Competences of Oocytes and Embryo Culture

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4098 - 4098

Опубликована: Апрель 25, 2025

Mitochondria, as multifunctional and partially independent structures, play a crucial role in determining essential life processes. Recently, their significance reproductive biology has gained increasing attention. This review aims to comprehensively analyse the of mitochondrial processes oocyte maturation embryo culture. A comprehensive literature was conducted highlight importance activity early stages formation. Proper function provides energy, maintains genomic stability, ensures optimal conditions for fertilisation progression. Understanding these is optimise culture identify new biomarkers that improve success assisted technologies (ARTs). Enhancing female cells key improving quality, which can lead better vitro transfer. Furthermore, advances diagnostic techniques, such genome sequencing, offer more precise understanding relationship between health quality. However, fully functions only part challenge. Expanding knowledge interactions mitochondria other cellular structures future advancements medicine. complex relationships will provide deeper insight into outcomes development.

Язык: Английский

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Revealing the Landscape Crosstalk Between Reproductive System and Organs Aging

The FASEB Journal, Год журнала: 2025, Номер 39(8)

Опубликована: Апрель 27, 2025

ABSTRACT The reproductive system is a vital component of the human body. In modern society, due to various socio‐economic reasons, an increasing number couples are choosing postpone childbearing. Research into impact aging on becoming increasingly important. As people age, there decline in across levels, from testes males spermatogonia cells, and ovaries females oocytes. not only affects itself but also has implications for other organs systems Conversely, can damage system. This review organizes changes that occur within as result focuses interactions between systems. Additionally, this summarizes current therapies aimed at delaying aging, which may provide insights future interventions targeting

Язык: Английский

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Dysregulation of alternative splicing patterns in the ovaries of reproductively aged mice

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 21, 2025

Abstract Female reproductive aging is marked by a decline in oocyte quality, yet the underlying molecular mechanisms remain incompletely understood. Here, we used long-read direct RNA-sequencing to map transcript isoform changes mouse ovaries across age. Comparing young and aged mice under controlled gonadotropin stimulation, identified widespread alternative splicing changes, including shifts exon usage, splice site selection, boundaries. Aged exhibited increased diversity, favoring distal start end sites, significant rise skipping intron retention events. Many of these age-biased events altered open reading frames, introduced premature stop codons, or disrupted conserved protein domains. Notably, mitochondrial genes were disproportionately affected. We highlight Ndufs4 , Complex I subunit, as case which promotes truncated lacking canonical Pfam domain. Structural modeling suggests this variant could impair assembly, providing mechanistic link between dysfunction ovary. These findings support existence splicing-energy-aging axis ovarian physiology, wherein declining function adaptive maladaptive are intertwined. Our study reveals that not merely byproduct but dynamic, transcriptome-wide regulatory layer may influence competence longevity. insights new avenues for investigating post-transcriptional underscore need consider isoform-level regulation models decline. In brief Reproductive associated with patterns identifies novel transcripts resulting from alterations usage have important roles physiology well aging.

Язык: Английский

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On the role of artificial intelligence in analysing oocytes during in vitro fertilisation procedures

Artificial Intelligence in Medicine, Год журнала: 2024, Номер 157, С. 102997 - 102997

Опубликована: Окт. 8, 2024

Nowadays, the most adopted technique to address infertility problems is in vitro fertilisation (IVF). However, its success rate limited, and associated procedures, known as assisted reproduction technology (ART), suffer from a lack of objectivity at laboratory level clinical practice. This paper deals with applications Artificial Intelligence (AI) techniques IVF procedures. intelligence considered promising tool for ascertaining quality embryos, critical step IVF. Since oocyte influences final embryo quality, we present systematic review literature on AI-based used assess quality; analyse results discuss several research directions. In particular, highlight how can support process examine their current presented literature. Then, challenges must face fully deploying solutions medical Among them, availability high-quality data sets well standardised imaging protocols formats, use physics-informed simulation machine learning techniques, study informative, descriptive yet observable features, and, above all, studies oocytes specifically about live birth potential. An improved understanding determinants improve rates while reducing costs, risks long-term cultures, bioethical concerns.

Язык: Английский

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Endocrine-Disrupting Chemicals and Female Reproductive Aging

Seminars in Reproductive Medicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Female reproductive aging often affects women's emotional, physical, and physiological well-being. Ovarian is characterized by fluctuations in hormones determines the age at which menopause occurs. Understanding potentially modifiable factors that influence this process essential for addressing health disparities, improving quality of life, informing relevant public strategies. This review synthesizes vivo, vitro, epidemiological findings about effects endocrine-disrupting chemicals (EDCs), specifically heavy metals perfluoroalkyl polyfluoroalkyl substances (PFAS) on female aging. Most vivo vitro studies have demonstrated alter ovarian morphology, folliculogenesis, steroidogenesis. Evidence regarding PFAS limited inconsistent. Epidemiological consistently shown are associated with a higher risk diminished reserve (indicated decreased anti-Müllerian hormone) earlier menopause, hormone changes. exposure has been estradiol but not significantly reserve. Gaps literature require more comprehensive research EDCs aging, including reserve, vasomotor symptoms, to inform future interventions reduce hazardous exposures improve health.

Язык: Английский

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Folicular dynamics, endocrine profiles and endometrial gene expression in fertile and subfertile cows

Reproduction Fertility and Development, Год журнала: 2025, Номер 37(4)

Опубликована: Фев. 2, 2025

Context Subfertility has a negative impact on the productivity of beef cow herds; thus, it is relevant to unravel mechanisms. Aims To study follicular development, body composition, concentrations progesterone and metabolic hormones their gene expression in endometrium during oestrus cycle. Methods Fertile subfertile Hereford cows were classified at 25 (n =84) 60 =25) months old, as (1) fertile, pregnant first insemination 25months, that became pregnantmore than three five times 60months =5), or (2) subfertile, i.e. failed inseminations fewer two opportunities =6). Key results had greater insuling-like growth factor 1 (IGF-1), leptin adiponectin (P P Conclusions Differences plasma fluid endometrial are associated with success pregnancy cows. Implications Strategies improve follicle microenvironments needed herds.

Язык: Английский

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Pyruvate Regulates the Expression of DLAT to Promote Follicular Growth

Cells, Год журнала: 2025, Номер 14(6), С. 444 - 444

Опубликована: Март 17, 2025

Increasing evidence has suggested that dihydrolipoamide S-acetyltransferase (DLAT), a subunit of the pyruvate dehydrogenase complex, is crucial for metabolism and regulation cell death. The excessive death granulosa cells (GCs) hinders progression follicular growth. However, relationship between DLAT growth poorly understood. Here, we found significantly shortened age pubertal initiation in mice promoted by promoting proliferation GCs. In addition, up-regulated expression high level was observed large follicles, which were associated with Mechanistically, increased mRNA protein levels pathways such as PCNA MCL1 to promote GC proliferation. Additionally, bound CASP3 CASP9 proteins inhibit apoptosis Taken together, these results reveal mechanism regulated growth, represents promising target supports new strategies improving follicles.

Язык: Английский

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