Journal of the American Heart Association,
Год журнала:
2024,
Номер
13(9)
Опубликована: Апрель 19, 2024
Cellular
therapies
have
been
investigated
to
improve
blood
flow
and
prevent
amputation
in
peripheral
artery
disease
with
limited
efficacy
clinical
trials.
Alginate-encapsulated
mesenchymal
stromal
cells
(eMSCs)
demonstrated
improved
retention
survival
promoted
vascular
generation
murine
hind
limb
ischemia
through
their
secretome,
but
large
animal
evaluation
is
necessary
for
human
applicability.
We
sought
determine
the
of
eMSCs
disease-induced
assessment
our
durable
swine
model.
Abstract
Regeneration
is
a
complex
process
affected
by
many
elements
independent
or
combined,
including
inflammation,
proliferation,
and
tissue
remodeling.
Stem
cells
class
of
primitive
with
the
potentiality
differentiation,
regenerate
self‐replication,
multidirectional
immunomodulatory
functions.
their
cytokines
not
only
inextricably
linked
to
regeneration
ectodermal
skin
tissues,
but
also
can
be
used
for
treatment
variety
chronic
wounds.
produce
exosomes
in
paracrine
manner.
cell
play
an
important
role
regeneration,
repair,
accelerated
wound
healing,
biological
properties
which
are
similar
stem
cells,
while
safer
more
effective.
Skin
bone
tissues
critical
organs
body,
essential
sustaining
life
activities.
The
weak
repairing
ability
leads
pronounced
impact
on
quality
patients,
could
alleviated
treatment.
However,
there
obstacles
that
trough
improved
bioavailability.
This
paper
summarizes
applications
mechanisms
healing.
We
propose
new
ways
utilizing
through
different
nanoformulations,
liposomes
nanoliposomes,
polymer
micelles,
microspheres,
hydrogels,
scaffold
microneedles,
improve
use
healing
regeneration.
International Journal of Nanomedicine,
Год журнала:
2022,
Номер
Volume 17, С. 3893 - 3911
Опубликована: Сен. 1, 2022
Abstract:
The
recent
rapid
development
in
the
field
of
extracellular
vesicles
(EVs)
based
nanotechnology
has
provided
unprecedented
opportunities
for
nanomedicine
platforms.
As
natural
nanocarriers,
EVs
such
as
exosomes,
exosome-like
nanoparticles
and
outer
membrane
(OMVs),
have
unique
structure/composition/morphology
characteristics,
show
excellent
physical
chemical/biochemical
properties,
making
them
a
new
generation
theranostic
nanomedicine.
Here,
we
reviewed
characteristics
from
perspective
their
formation
biological
function
inflammatory
bowel
disease
(IBD).
Moreover,
can
crucially
participate
interaction
communication
intestinal
epithelial
cells
(IECs)-immune
cells-gut
microbiota
to
regulate
immune
response,
inflammation
homeostasis.
Interestingly,
on
current
representative
examples
exosomes
IBD
treatment,
it
is
shown
that
plant,
milk,
cells-derived
exert
therapeutic
effect
through
components,
proteins,
nucleic
acid,
lipids.
several
drug
loading
methods
target
modification
are
used
improve
capability.
We
also
discussed
application
treatment
IBD.
In
this
review,
aim
better
more
clearly
clarify
underlying
mechanisms
pathogenesis
IBD,
provide
directions
mediated
treatment.
Keywords:
vesicles,
exosome,
nanoparticles,
disease,
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(5), С. 2335 - 2335
Опубликована: Фев. 26, 2021
Critical
limb
ischemia
(CLI)
constitutes
the
most
severe
form
of
peripheral
arterial
disease
(PAD),
it
is
characterized
by
progressive
blockade
vessels,
commonly
correlated
to
atherosclerosis.
Currently,
revascularization
strategies
(bypass
grafting,
angioplasty)
remain
first
option
for
CLI
patients,
although
less
than
45%
them
are
eligible
surgical
intervention
mainly
due
associated
comorbidities.
Moreover,
patients
usually
require
amputation
in
short-term.
Angiogenic
cell
therapy
has
arisen
as
a
promising
alternative
these
"no-option"
with
many
studies
demonstrating
potential
stem
cells
enhance
promoting
vessel
formation
and
blood
flow
recovery
ischemic
tissues.
Herein,
we
provide
an
overview
focused
on
use
angiogenic
therapies
last
years,
from
approaches
testing
different
types
animal/pre-clinical
models
CLI,
clinical
trials
currently
under
evaluation.
Furthermore,
recent
alternatives
related
such
secretomes,
exosomes,
or
even
microRNA,
will
be
also
described.
Frontiers in Endocrinology,
Год журнала:
2021,
Номер
12
Опубликована: Ноя. 29, 2021
Mesenchymal
stem
cell-derived
exosomes
have
been
under
investigation
as
potential
treatments
for
a
diverse
range
of
diseases,
and
many
animal
clinical
trials
achieved
encouraging
results.
However,
it
is
well
known
that
the
biological
activity
key
to
their
therapeutic
properties;
however,
till
date,
has
not
completely
understood.
Previous
studies
provided
different
explanations
mechanisms
exosomes,
including
anti-inflammatory,
immunomodulatory,
anti-aging
mechanisms.
The
pathological
effects
oxidative
stress
often
include
organ
damage,
inflammation,
disorders
material
energy
metabolism.
evidence
gathered
from
research
involving
models
indicates
antioxidant
properties,
which
can
also
explain
anti-inflammatory
cytoprotective
effects.
In
this
study,
we
summarized
in
vivo
vitro
models,
evaluated
anti-oxidant
by
demonstrating
direct
reduction
excessive
reactive
oxygen
species
(ROS),
promotion
intracellular
defence
anti-oxidative
stress,
immunomodulation
inhibiting
excess
ROS,
alteration
mitochondrial
performance.
Exosomes
exert
properties
regulating
redox
environment
explains
variety
be
preserved
among
species.
Journal of Materials Science Materials in Medicine,
Год журнала:
2022,
Номер
33(7)
Опубликована: Июнь 18, 2022
Abstract
Stem
cells
exist
as
normal
in
embryonic
and
adult
tissues.
In
recent
years,
scientists
have
spared
efforts
to
determine
the
role
of
stem
treating
many
diseases.
can
self-regenerate
transform
into
some
somatic
cells.
They
would
also
a
special
position
future
various
clinical
fields,
drug
discovery,
other
scientific
research.
Accordingly,
detection
safe
low-cost
methods
obtain
such
is
one
main
objectives
Jaw,
face,
mouth
tissues
are
rich
sources
cells,
which
more
accessible
than
so
cell
tissue
engineering
treatments
dentistry
received
much
attention
years.
This
review
study
examines
three
essential
elements
practice,
including
derived
from
intra-
extra-oral
sources,
growth
factors,
scaffolds.
Medicina,
Год журнала:
2023,
Номер
59(8), С. 1449 - 1449
Опубликована: Авг. 10, 2023
Soft
tissue
regeneration
holds
significant
promise
for
addressing
various
clinical
challenges,
ranging
from
craniofacial
and
oral
defects
to
blood
vessels,
muscle,
fibrous
regeneration.
Mesenchymal
stem
cells
(MSCs)
have
emerged
as
a
promising
tool
in
regenerative
medicine
due
their
unique
characteristics
potential
differentiate
into
multiple
cell
lineages.
This
comprehensive
review
explores
the
role
of
MSCs
different
aspects
soft
regeneration,
including
application
nerve
vessel
muscle
By
examining
latest
research
findings
advancements,
this
article
aims
provide
insights
current
state
MSC-based
therapies
medicine.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Май 14, 2024
Abstract
Background
Perianal
fistulas
(PF)
affect
one-third
patients
with
Crohn’s
disease
(CD)
limited
therapeutic
options.
There
is
dearth
of
literature
on
safety
and
efficacy
bone
marrow-derived
mesenchymal
stromal
cells
(BMSCs)
in
this
population.
Methods
An
open-label,
phase
I/II,
single-arm
study
was
conducted
involving
local
administration
human
allogeneic
perianal
fistula
refractory
to
standard
therapies.
Clinical
severity
biomarkers
were
assessed
at
baseline
periodically
until
week
104
,
MRI
24
104.
Primary
secondary
objectives
assess
respectively.
Fistula
remission
complete
closure
openings
<
2
cm
collection
MRI,
response
decrease
drainage
by
≥
50%.
Change
activity
index,
quality-of-life
Van
Assche
index
over
time
using
mixed-effect
linear
regression
model.
Results
Ten
(male:8,
mean
age:27.4
±
12.0years)
recruited.
Self-resolving
procedure-related
adverse
events
occurred
three
patients,
no
follow-up
events.
In
intention
treat
analysis
24,
two
(20%)
achieved
seven
(70%)
had
response.
At
52,
maintained
weeks,
one
(10%)
remission.
Statistically
significant
(
P
=
0.008),
Index
0.008)
improvement
0.001)
observed
time.
Conclusions
Allogeneic
BMSCs
are
safe
effective
for
the
treatment
fistulizing
CD
clinical
radiological
healing.
Trial
registration
The
prospectively
registered
trials
registry
–
India
(CTRI),
CTRI/2020/01/022743
14
January
2020,
http://ctri.nic.in
.
Stem Cell Research & Therapy,
Год журнала:
2022,
Номер
13(1)
Опубликована: Июль 16, 2022
Abstract
Background
Pulmonary
arterial
hypertension
(PAH)
is
associated
with
oxidative
stress
and
affects
the
survival
homing
of
transplanted
mesenchymal
stem
cells
(MSCs)
as
well
cytokine
secretion
by
MSCs,
thereby
altering
their
therapeutic
potential.
In
this
study,
we
preconditioned
MSCs
prostaglandin
E1
(PGE1)
performed
in
vitro
vivo
cell
experiments
to
evaluate
effects
rats
PAH.
Methods
We
studied
relationship
between
PGE1
vascular
endothelial
growth
factor
(VEGF)
secretion,
B-cell
lymphoma
2
(Bcl-2)
expression,
C-X-C
chemokine
receptor
4
(CXCR4)
expression
MSC
apoptosis
migration
through
hypoxia-inducible
(HIF)
pathway
vitro.
The
experimental
were
randomly
divided
into
five
groups:
(I)
control
group,
(II)
monocrotaline
(MCT)
(III)
MCT
+
non-preconditioned
(Non-PC)
(IV)
PGE1-preconditioned
(PGE1-PC)
(V)
+PGE1+YC-1-PC
group.
methane
dicarboxylic
aldehyde
(MDA)
levels,
rat
lungs,
mean
pulmonary
artery
pressure,
systolic
right
ventricular
hypertrophy
index,
wall
thickness
index
(%WT),
relative
area
(%WA)
arterioles.
Results
Preconditioning
increased
protein
levels
HIF-1
alpha
(HIF-1α)
which
can
reduce
increase
CXCR4,
migration,
secretion.
Upon
injection
PGE1-PC
%WT,
%WA
decreased
exhibited
better
than
non-PC
MSCs.
Interestingly,
lificiguat
(YC-1),
an
inhibitor
HIF
pathway,
blocked
preconditioning.
Conclusions
Our
findings
indicate
that
modulates
properties
regulating
providing
insights
mechanism
preconditioning
be
used
improve
potential
