Bagcilar Medical Bulletin,
Год журнала:
2023,
Номер
8(2), С. 113 - 126
Опубликована: Май 3, 2023
Degenerative
joint
disease
is
a
condition
that
affects
joints
and
commonly
referred
to
as
osteoarthritis
(OA).This
form
of
arthritis
most
prevalent
among
women
tends
become
more
frequent
people
age.The
pathogenesis
OA
involves
an
imbalance
cytokines
in
favor
pro-inflammatory
cytokines.However,
the
steroid
hormone
dehydroepiandrosterone
(DHEA)
exerts
chondroprotective
effects
regulates
balance
catabolic
factors
such
thrombospondin
motif
disintegrin
metalloproteinase
(ADAMTS),
thereby
playing
role
against
OA.Pro-inflammatory
induce
aggrecanases,
ADAMTS5,
which
degrade
extracellular
matrix
contribute
OA.The
molecule
nerve
growth
factor
(NGF),
associated
with
pain
OA,
important
for
cartilage
homeostasis,
DHEA
can
modulate
by
interfering
NGF
receptors.This
review
covers
roles
DHEA,
their
relationship
pathways,
use
current
treatments.We
also
anticipate
these
pathways
will
be
crucial
developing
new
strategies
prevent
treat
understanding
interactions
may
make
it
possible
enhanced
quality
life
patients
OA.
International Journal of Biological Sciences,
Год журнала:
2024,
Номер
20(8), С. 2994 - 3007
Опубликована: Янв. 1, 2024
Osteoarthritis
(OA)
is
a
challenging
degenerative
joint
disease
to
manage.
Previous
research
has
indicated
that
cell-free
fat
extract
(CEFFE)
may
hold
potential
for
OA
treatment.
This
study
investigated
the
role
of
Annexin
A5
(AnxA5)
within
CEFFE
in
regulating
macrophage
polarization
and
protecting
chondrocytes.
The
reconstruction
of
a
stable,
nipple-shaped
cartilage
graft
that
precisely
matches
the
natural
nipple
in
shape
and
size
on
contralateral
side
is
clinical
challenge.
While
3D
printing
technology
can
efficiently
accurately
manufacture
customized
complex
structures,
it
faces
limitations
due
to
inadequate
blood
supply,
which
hampers
stability
grafts
produced
using
this
technology.
To
address
issue,
we
employed
biodegradable
biomaterial,
Poly(lactic-co-glycolic
acid)
(PLGA),
loaded
with
Cell-Free
Fat
Extract
(Ceffe).
Ceffe
has
demonstrated
ability
promote
angiogenesis
cell
proliferation,
making
an
ideal
bio-ink
for
bioprinting
precise
grafts.
We
utilized
Ceffe/PLGA
scaffold
create
porous
structure
shape.
This
exhibited
favorable
porosity
pore
size,
ensuring
stable
maintenance
satisfactory
biomechanical
properties.
Importantly,
could
release
sustained
manner.
Our
vitro
results
confirmed
scaffold's
good
biocompatibility
its
angiogenesis,
as
evidenced
by
supporting
chondrocyte
proliferation
endothelial
migration
tube
formation.
Furthermore,
after
8
weeks
vivo
culture,
seeded
chondrocytes
regenerated
into
support
Compared
pure
PLGA
group,
showed
remarkable
vascular
formation,
highlighting
beneficial
effects
Ceffe.
These
findings
suggest
our
designed
represents
promising
strategy
regeneration,
laying
foundation
subsequent
reconstruction.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(12), С. 9828 - 9828
Опубликована: Июнь 6, 2023
Female
infertility
and
reproduction
is
an
ongoing
rising
healthcare
issue,
resulting
in
delaying
the
decision
to
start
a
family.
Therefore,
this
review,
we
examine
potential
novel
metabolic
mechanisms
involved
ovarian
aging
according
recent
data
how
these
may
be
addressed
through
new
medical
treatments.
We
treatments
currently
available
based
mostly
on
experimental
stem
cell
procedures
as
well
caloric
restriction
(CR),
hyperbaric
oxygen
treatment
mitochondrial
transfer.
Understanding
connection
between
reproductive
pathways
has
offer
significant
scientific
breakthrough
preventing
prolonging
female
fertility.
Overall,
field
of
emerging
that
expand
fertility
window
perhaps
even
reduce
need
for
artificial
techniques.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
11
Опубликована: Июнь 22, 2023
Background:
The
regenerative
capabilities
of
derivatives
derived
from
the
fat
layer
lipoaspirate
have
been
demonstrated.
However,
large
volume
fluid
has
not
attracted
extensive
attention
in
clinical
applications.
In
this
study,
we
aimed
to
isolate
factors
and
extracellular
vesicles
human
evaluate
their
potential
therapeutic
efficacy.
Methods:
Lipoaspirate
(LF-FVs)
were
prepared
characterized
by
nanoparticle
tracking
analysis,
size-exclusion
chromatography
adipokine
antibody
arrays.
LF-FVs
was
evaluated
on
fibroblasts
vitro
rat
burn
model
vivo.
Wound
healing
process
recorded
days
2,
4,
8,
10,
12
16
post-treatment.
scar
formation
analyzed
histology,
immunofluorescent
staining
scar-related
gene
expression
at
day
35
Results:
results
analysis
indicated
that
enriched
with
proteins
vesicles.
Specific
adipokines
(adiponectin
IGF-1)
detected
LF-FVs.
vitro,
augmented
proliferation
migration
a
dose-dependent
manner.
vivo,
showed
significantly
accelerated
wound
healing.
Moreover,
improved
quality
healing,
including
regenerating
cutaneous
appendages
(hair
follicles
sebaceous
glands)
decreasing
healed
skin.
Conclusion:
successfully
liquid,
which
cell-free
Additionally,
they
found
improve
model,
suggesting
could
be
potentially
used
for
regeneration
settings.
The FASEB Journal,
Год журнала:
2024,
Номер
38(14)
Опубликована: Июль 12, 2024
Abstract
High‐quality
fat
(HQF)
improves
the
survival
rate
of
and
volumetric
filling
compared
to
traditional
Coleman
fat.
However,
this
HQF
strategy
inevitably
leads
a
significant
amount
unused
being
wasted.
“CEFFE”
(cell‐free
extract)
is
an
acellular
aqueous‐phase
liquid,
rich
in
bioactive
proteins.
The
remaining
from
preparing
can
be
further
processed
into
CEFFE
promote
HQF.
was
obtained
CEFFE,
then
transplanted
subcutaneously
nude
mice.
Animal
studies
showed
that
significantly
improved
Histological
analysis
revealed
HQF,
by
enhancing
cell
proliferation
activity,
reducing
apoptosis,
increasing
angiogenesis,
improving
inflammatory
state.
Under
simulated
anaerobic
conditions,
also
viability
In
vitro,
demonstrated
enhanced
through
multiple
mechanisms.
Transcriptomic
qPCR
increased
expression
angiogenesis‐related
genes
ADSCs
while
their
proliferation‐related
gene
suppressing
three
differentiation‐related
genes.
Moreover,
functional
experiments
CEFFE‐induced
exhibited
stronger
adipogenic
differentiation
abilities.
Tube
formation
migration
assays
promoted
tube
HUVECs,
indicating
its
inherent
pro‐angiogenic
properties.
facilitated
development
M0
M2
macrophages,
suggesting
role
This
innovative
clinical
optimizes
transplantation
strategy,
minimizing
wastage
efficiency
utilization.
Bali Medical Journal,
Год журнала:
2023,
Номер
12(1), С. 899 - 903
Опубликована: Март 1, 2023
Link
of
Video
Abstract:
https://youtu.be/XrtI29YbnTs
Background:
Osteoarthritis
(OA)
is
the
most
common
form
arthritis,
which
cause
pain
and
disability
that
can
affect
quality
life.
The
inflammation
in
OA
chronic
characterized
by
infiltration
immune
cells
secretion
cytokines.
Previous
treatment
used
hyaluronic
acid
intraarticular
injection,
but
several
studies
show
no
satisfactory
clinical
outcome.
This
study
aims
to
determine
compare
anti-inflammatory
anti-catabolism
effects
stem
cell-derived
secretomes
versus
as
gold
standard
for
OA.
Methods:
research
was
conducted
using
an
experimental
randomized
post-test-only
control
group
design.
According
veterinarians,
population
this
were
local
rabbits,
aged
6-8
months,
weighing
>
1,500
grams,
healthy.
Data
analyzed
SPSS
version
22.0
Windows.
Results:
Twenty-two
subjects
included
each
group.
From
descriptive
analysis,
it
found
MMP-13
levels
7.55
±
0.23
(Treatment)
(ng/mL)
8.36
1.04
(Control)
(ng/mL),
ADAMTS-5
0.83
0.08
1.14
0.33
IL-1β
23.0
5±
2.48
26.81
5.57
(ng/mL).
inferential
test,
all
results
statistically
significant
(MMP-13=
0.81;
p
=
0.021;
95%CI
-1.48
–
(-0.13))
(Agrekanase
2
0.32;
0.006;
95%
CI
-0.53
(-0.09))
(IL-1β
3.76;
0.023;
-7.59
(-0.08)).
Conclusion:
Administration
mesenchymal
resulted
significantly
lower
MMP-13,
Agrekanase
2,
compared
administration
rabbit
knee
osteoarthritis
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 22, 2024
Abstract
The
reconstruction
of
a
stable,
nipple-shaped
cartilage
graft
that
precisely
matches
the
natural
nipple
in
shape
and
size
on
contralateral
side
is
clinical
challenge.
While
3D
printing
technology
can
efficiently
accurately
manufacture
customized
complex
structures,
it
faces
limitations
due
to
inadequate
blood
supply,
which
hampers
stability
grafts
produced
using
this
technology.
To
address
issue,
we
employed
biodegradable
biomaterial,
Poly(lactic-co-glycolic
acid)
(PLGA),
loaded
with
Cell-Free
Fat
Extract
(Ceffe).
Ceffe
has
demonstrated
ability
promote
angiogenesis
cell
proliferation,
making
an
ideal
bio-ink
for
bioprinting
precise
grafts.
We
utilized
Ceffe/PLGA
scaffold
create
porous
structure
shape.
This
exhibited
favorable
porosity
pore
size,
ensuring
stable
maintenance
satisfactory
biomechanical
properties.
Importantly,
could
release
sustained
manner.
Our
vitro
results
confirmed
scaffold's
good
biocompatibility
its
angiogenesis,
as
evidenced
by
supporting
chondrocyte
proliferation
endothelial
migration
tube
formation.
Furthermore,
after
8
weeks
vivo
culture,
seeded
chondrocytes
regenerated
into
support
Compared
pure
PLGA
group,
showed
remarkable
vascular
formation,
highlighting
beneficial
effects
Ceffe.
These
findings
suggest
our
designed
represents
promising
strategy
regeneration,
laying
foundation
subsequent
reconstruction.
