Organoids,
Год журнала:
2024,
Номер
3(3), С. 203 - 246
Опубликована: Сен. 4, 2024
Recent
advancements
in
vascular
organoid
(VO)
and
vessel-on-chip
(VoC)
technologies
have
revolutionized
our
approach
to
studying
human
diseases,
offering
unprecedented
insights
through
more
physiologically
relevant
models.
VOs
generated
from
pluripotent
stem
cells
exhibit
remarkable
self-organization
capabilities,
forming
complex
three-dimensional
structures
that
closely
mimic
blood
vessel
architecture
function,
while
VoCs
are
engineered
with
microfluidic
systems
meticulously
recreate
the
physical
functional
attributes
of
vessels.
These
innovative
constructs
serve
as
powerful
tools
for
investigating
development,
disease
progression,
therapeutic
efficacy.
By
enabling
creation
patient-specific
VoCs,
they
pave
way
personalized
medicine
approaches,
allowing
researchers
delve
into
genetic
variations,
intricate
cellular
interactions,
dynamic
processes
exceptional
resolution.
The
synergy
between
newly
developed
cutting-edge
has
further
amplified
their
potential,
unveiling
novel
mechanisms
underlying
pathologies
identifying
promising
targets.
Herein,
we
summarize
different
types
present
an
extensive
overview
on
generation
applications
VoCs.
We
will
also
highlight
clinical
translational
challenges
future
perspectives
around
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(2), С. 993 - 993
Опубликована: Янв. 12, 2024
Regenerative
medicine
harnesses
the
body's
innate
capacity
for
self-repair
to
restore
malfunctioning
tissues
and
organs.
Stem
cell
therapies
represent
a
key
regenerative
strategy,
but
effectively
harness
their
potential
necessitates
nuanced
understanding
of
stem
niche.
This
specialized
microenvironment
regulates
critical
behaviors
including
quiescence,
activation,
differentiation,
homing.
Emerging
research
reveals
that
dysfunction
within
endogenous
neural
niches
contributes
neurodegenerative
pathologies
impedes
regeneration.
Strategies
such
as
modifying
signaling
pathways,
or
epigenetic
interventions
niche
homeostasis
signaling,
hold
promise
revitalizing
neurogenesis
repair
in
diseases
like
Alzheimer's
Parkinson's.
Comparative
studies
highly
species
provide
evolutionary
clues
into
niche-mediated
renewal
mechanisms.
Leveraging
bioelectric
cues
crosstalk
between
gut,
brain,
vascular
further
illuminates
promising
therapeutic
opportunities.
techniques
single-cell
transcriptomics,
organoids,
microfluidics,
artificial
intelligence,
silico
modeling,
transdifferentiation
will
continue
unravel
complexity.
By
providing
comprehensive
synthesis
integrating
diverse
views
on
components,
developmental
transitions,
dynamics,
this
review
unveils
new
layers
complexity
integral
behavior
function,
which
unveil
novel
prospects
modulate
function
revolutionary
treatments
diseases.
Frontiers in Medicine,
Год журнала:
2025,
Номер
11
Опубликована: Янв. 24, 2025
The
liver
is
a
vital
organ
responsible
for
numerous
metabolic
processes
in
the
human
body,
including
metabolism
of
drugs
and
nutrients.
After
damage,
can
rapidly
return
to
its
original
size
if
causative
factor
promptly
eliminated.
However,
when
harmful
stimulus
persists,
liver’s
regenerative
capacity
becomes
compromised.
Substantial
theoretical
feasibility
has
been
demonstrated
at
levels
gene
expression,
molecular
interactions,
intercellular
dynamics,
complemented
by
successful
animal
studies.
robust
model
carrier
that
closely
resemble
physiology
are
still
lacking
translating
these
theories
into
practice.
potential
regeneration
central
focus
ongoing
research.
Over
past
decade,
advent
organoid
technology
provided
improved
models
materials
advancing
research
efforts.
Liver
represents
novel
vitro
culture
system.
several
years
refinement,
organoids
now
accurately
replicate
morphological
structure,
nutrient
drug
metabolism,
secretory
functions,
providing
disease
Regenerative
medicine
aims
or
tissue
functions
repair
replace
damaged
tissues,
restore
their
structure
function,
stimulate
tissues
organs
within
body.
possess
same
function
as
tissue,
offering
serve
viable
replacement
liver,
aligning
with
goals
medicine.
This
review
examines
role
Abstract
In
the
past
few
years,
emergence
of
organoids
and
organ-on-a-chip
(OOAC)
technologies,
which
are
complementary
to
animal
models
two-dimensional
cell
culture
methods
can
better
simulate
internal
environment
human
body,
provides
a
new
platform
for
traditional
Chinese
medicine
(TCM)
studies.
Organoids
OOAC
techniques
have
been
increasingly
applied
in
fields
drug
screening,
assessment
development,
personalized
therapies,
developmental
biology,
there
some
application
cases
TCM
this
review,
we
summarized
current
status
using
organoid
technologies
research
provide
key
insights
future
study.
It
is
believed
that
will
play
more
important
roles
make
greater
contributions
innovative
development
TCM.
Virtual and Physical Prototyping,
Год журнала:
2024,
Номер
19(1)
Опубликована: Авг. 19, 2024
Tissue
engineering,
an
interdisciplinary
field,
aims
to
restore,
maintain,
or
enhance
tissue
function
by
developing
biological
substitutes.
To
establish
optimal
microenvironment,
biofabrication
is
acknowledged
as
a
revolutionary
technology
facilitating
the
construction
of
multiscale
three-dimensional
architectures
utilising
various
living
cells
and
biomaterials.
A
critical
challenge
in
this
domain
precise
assembly
soft
matter-delicate
pliable
structures
-
essential
for
creating
functional
tissues
organs.
This
review
focuses
on
increasing
use
robotic
systems
address
challenge,
exploring
their
application
manipulating
matter
assembly.
Robotic
technologies
offer
innovative
solutions
handling
with
precision
control.
discusses
how
can
be
leveraged
fabricate
complex
organs,
detailing
role
replicating
structural
complexities
natural
tissues.
Further,
examining
latest
advancements
robotics-assisted
highlights
potential
robotics
revolutionise
organ
fabrication,
offering
promising
longstanding
challenges
field.
These
technological
hold
capacity
engineer
presenting
considerable
translational
clinical
applications.
Endosteal
bone
marrow
(BM)
niches
are
crucial
to
sustain
non-steady-state
hematopoiesis
but
challenging
be
modelled
in
their
cellular
and
molecular
complexity
standardized,
human
settings.
We
report
a
developmentally-guided
approach
generate
macro-scale
organotypic
model
of
BM
endosteal
(engineered
vascularized
osteoblastic
niche,
eVON)
based
on
induced
pluripotent
stem
cells
(hiPSC)
porous
hydroxyapatite
scaffolds.
Vascular
derived
from
the
same
hiPSC
self-assembled
into
complex
long-lasting
vascular
networks
integrated
within
osteogenic
matrix.
The
system
supported
vitro
was
stable
upon
implantation
vivo.
Transcriptomic
analysis
revealed
osteogenic,
neural
expressing
key
niche
signals
(e.g.,
CXCL12,
KITLG
VEGFA)
human-specific
patterns.
eVON
could
perturbed
at
(removing
cells)
(deregulating
VEGF
signaling)
levels
study
contribution
vasculature
myelopoiesis.
offers
unprecedented
possibilities
dissect
pathophysiological
BM.
Abstract
The
formation
of
a
blood
vessel
network
is
crucial
for
organ
development
and
regeneration.
Over
the
past
three
decades,
central
molecular
mechanisms
governing
growth
have
been
extensively
studied.
Recent
evidence
indicates
that
vascular
endothelial
cells—the
specialized
cells
lining
inner
surface
vessels—exhibit
significant
heterogeneity
to
meet
specific
needs
different
organs.
This
review
focuses
on
current
understanding
cell
heterogeneity,
which
includes
both
intra-organ
inter-organ
heterogeneity.
Intra-organ
encompasses
arterio-venous
tip-stalk
specialization,
while
refers
organ-specific
transcriptomic
profiles
functions.
Advances
in
single-cell
RNA
sequencing
(scRNA-seq)
enabled
identification
new
subpopulations
comparison
gene
expression
patterns
across
subsets
cells.
Integrating
scRNA-seq
with
other
high-throughput
technologies
promises
deepen
our
at
epigenetic
level
spatially
resolved
context.
To
further
explore
human
organoids
offer
powerful
tools
studying
function
three-dimensional
culture
systems
investigating
endothelial-tissue
interactions
using
Developing
presents
unique
opportunities
unravel
its
implications
disease.
Emerging
technologies,
such
as
organoids,
are
poised
transform
pave
way
innovative
therapeutic
strategies
address
diseases.
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