Current Understanding and Translational Prospects of Tetrahedral Framework Nucleic Acids

JACS Au, Год журнала: 2025, Номер 5(2), С. 486 - 520

Опубликована: Фев. 10, 2025

Tetrahedral framework nucleic acids (tFNAs) represent a promising advancement in acid nanotechnology due to their unique structural properties, high biocompatibility, and multifaceted biomedical applications. Constructed through one-pot annealing method, four single-stranded DNAs self-assemble into stable, three-dimensional tetrahedral nanostructures with enhanced mechanical robustness physiological stability, resisting enzymatic degradation. Their ability permeate mammalian cells without transfection agents, coupled modifiable surfaces, positions tFNAs as versatile carriers for drug gene delivery systems. The tFNA-based platforms exhibit superior therapeutic efficacy, including antioxidative anti-inflammatory effects, alongside efficient cellular uptake tissue penetration. These features underpin role precision medicine, enabling targeted of diverse agents such synthetic compounds, peptides, acids. Additionally, demonstrate significant potential regenerative immune modulation, antibacterial strategies, oncology. By addressing challenges translational integration, stand poised accelerate the development research clinical applications, fostering novel therapies enhancing outcomes across wide spectrum diseases. This Perspective thoroughly details attributes applications critically evaluates tFNAs' potential, outlining inherent implementation exploring solutions these obstacles.

Язык: Английский

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MicroRNAs in Parkinson’s disease: From pathogenesis to diagnostics and therapeutic strategies

Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Hydrogels for Peripheral Nerve Repair: Emerging Materials and Therapeutic Applications

Gels, Год журнала: 2025, Номер 11(2), С. 126 - 126

Опубликована: Фев. 9, 2025

Peripheral nerve injuries pose a significant clinical challenge due to the complex biological processes involved in repair and their limited regenerative capacity. Despite advances surgical techniques, conventional treatments, such as autografts, are faced with limitations like donor site morbidity inconsistent functional outcomes. As such, there is growing interest new, novel, innovative strategies enhance regeneration. Tissue engineering/regenerative medicine its use of biomaterials an emerging example strategy. Within realm tissue engineering, functionalized hydrogels have gained considerable attention ability mimic extracellular matrix, support cell growth differentiation, even deliver bioactive molecules that can promote repair. These be engineered incorporate factors, peptides, stem cells, creating conducive microenvironment for cellular axonal Recent advancements materials well biology led development sophisticated hydrogel systems, not only provide structural support, but also actively modulate inflammation, recruitment, stimulate neurogenesis. This review explores potential peripheral repair, highlighting composition, biofunctionalization, mechanisms action. A comprehensive analysis preclinical studies provides insights into efficacy these promoting growth, neuronal survival, regeneration, and, ultimately, recovery. Thus, this aims illuminate promise transformative tool field bridging gap between complexity feasibility.

Язык: Английский

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Nanotechnology to Overcome Blood–Brain Barrier Permeability and Damage in Neurodegenerative Diseases

Pharmaceutics, Год журнала: 2025, Номер 17(3), С. 281 - 281

Опубликована: Фев. 20, 2025

The blood–brain barrier (BBB) is a critical structure that maintains brain homeostasis by selectively regulating nutrient influx and waste efflux. Not surprisingly, it often compromised in neurodegenerative diseases. In addition to its involvement these pathologies, the BBB also represents significant challenge for drug delivery into central nervous system. Nanoparticles (NPs) have been widely explored as carriers capable of overcoming this effectively transporting therapies brain. However, their potential directly address ameliorate dysfunction has received limited attention. review, we examine how NPs enhance across treat diseases explore emerging strategies restore integrity vital structure.

Язык: Английский

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Advances in genetically modified neural stem cell therapy for central nervous system injury and neurological diseases

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 18, 2024

Neural stem cells (NSCs) have increasingly been recognized as the most promising candidates for cell-based therapies central nervous system (CNS) injuries, primarily due to their pluripotent differentiation capabilities, well remarkable secretory and homing properties. In recent years, extensive research efforts initiated explore therapeutic potential of NSC transplantation CNS yielding significant advancements. Nevertheless, owing formation adverse microenvironment at post-injury leading suboptimal survival, differentiation, integration within host neural network transplanted NSCs, NSC-based often fall short achieving optimal outcomes. To address this challenge, genetic modification has developed an attractive strategy improve outcomes therapies. This is mainly attributed its not only enhance capacity NSCs but also boost a range biological activities, such secretion bioactive factors, anti-inflammatory effects, anti-apoptotic properties, immunomodulation, antioxidative functions, angiogenesis. Furthermore, empowers play more robust neuroprotective role in context nerve injury. review, we will provide overview advances roles mechanisms genetically modified with various genes treatment injuries disorders. Also, update on current technical parameters suitable functional recovery clinical studies are summarized.

Язык: Английский

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Non-Drug and Non-Invasive Therapeutic Options in Alzheimer’s Disease

Biomedicines, Год журнала: 2025, Номер 13(1), С. 84 - 84

Опубликована: Янв. 1, 2025

Despite the massive efforts of modern medicine to stop evolution Alzheimer’s disease (AD), it affects an increasing number people, changing individual lives and imposing itself as a burden on families health systems. Considering that vast majority conventional drug therapies did not lead expected results, this review will discuss newly developing alternative in effort or slow AD. Focused Ultrasound (FUS) its derived Transcranial Pulse Stimulation (TPS) are non-invasive therapeutic approaches. Singly applied technique change permeability blood–brain–barrier (BBB), FUS TPS have demonstrated benefits use treating AD animal human studies. Adipose-derived stem Cells (ADSCs), gene therapy, many other methods (diet, sleep pattern, physical exercise, nanoparticle delivery) also new potential treatments since multimodal approaches represent trend disorder research therapies.

Язык: Английский

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In Vitro models of leukemia development: the role of very small leukemic stem-like cells in the cellular transformation cascade

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 12

Опубликована: Янв. 3, 2025

Recent experimental findings indicate that cancer stem cells originate from transformed very small embryonic-like cells. This finding represents an essential advancement in uncovering the processes drive onset and progression of cancer. In continuously growing cell lines, for first time, our team's follow-up research on leukemia, lung cancer, healthy embryonic kidney revealed stages resembles precursor review explores origin leukemic stem-like establish We explore theoretical model acute myeloid leukemia initiation progresses through various stages, as well basing HL60 line, present its hierarchical stage development vitro, highlighting role these primitive stages. also discuss potential implications further into unique cellular advancing treatment prevention.

Язык: Английский

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Advances in neural stem cells in aging and age-related neurodegenerative diseases

Ageing and Neurodegenerative Diseases, Год журнала: 2025, Номер 5(1)

Опубликована: Янв. 26, 2025

With aging, neural stem cells (NSCs) undergo age-related changes, including metabolic abnormalities, disrupted protein homeostasis, mitochondrial dysfunction, reduced genetic stability, and more notably, a diminished capacity for proliferation differentiation. These changes may contribute to the development of neurodegenerative diseases such as Alzheimer’s disease (AD) Parkinson’s (PD). In these conditions, disease-specific pathological interact with alterations NSCs, resulting in impairment neurogenesis, which further leads cognitive, mood, motor decline. Based on potential therapeutics targeting neurogenesis cell-based therapies restore functions NSCs replace degenerated neurons, aiming ameliorate functional decline diseases. While therapies, cell transplantation secretome therapy, show great treatment diseases, challenges tumorigenesis, immune rejection, extraction or storage need be addressed. A better understanding age- disease-related underlying mechanism driving benefits drawbacks therapeutic approaches provide insights novel disease-modifying interventions future

Язык: Английский

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Protective effects of harpagoside on mitochondrial functions in rotenone‑induced cell models of Parkinson's disease

Biomedical Reports, Год журнала: 2025, Номер 22(4)

Опубликована: Фев. 11, 2025

Parkinson's disease (PD) is the second most common neurodegenerative after Alzheimer's disease. Currently, no radical treatment available for this Harpagoside a proposed neuroprotective iridoid active ingredient that can be derived from Scrophulariae buergeriana, Scrophularia striata and Harpagophytum procumbens. The present study aimed to investigate effects of harpagoside on mitochondrial functions in rotenone-induced cell models (PD). Neuro-2A (N2A) cells were treated with rotenone establish vitro PD. Cell viability survival measured using Counting Kit-8 assay. Biochemical assays spectrophotometry used measure complex I activity, swelling caspase 3 activity. rate was first found significantly decreased by (20 nmol/l) treatment. However, intervention (10 µmol/l) increase N2A differentiated 1 mmol/l dibutyryl-cAMP. At ≥0.1 µmol/l concentration, alleviated swelling, whereas at it counteracted inhibitory 10 inhibited activation. These results suggest has potential protect against injury

Язык: Английский

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Unveiling the pH-Responsive Mechanisms of the Carbon Dot–Proximicin-A Peptide Conjugate for Targeted Cancer Therapy Using Density Functional Theory

Molecules, Год журнала: 2025, Номер 30(4), С. 896 - 896

Опубликована: Фев. 14, 2025

This study investigates the pH-responsive dissociation mechanism of carbon dot (CD) conjugated with anticancer peptide proximicin-A (PROXI) using density functional theory (DFT) simulations. The CD@PROXI system, designed for targeted cancer therapy, releases drug in acidic environments typical sites. DFT simulations, B3LYP-D3BJ and 6-311G (d, p) basis set, optimized conjugate's geometry under neutral conditions. focus was on pH-sensitive C=N bond, existing two protonation states. Key parameters evaluated included HOMO-LUMO gap, bond length, IR spectroscopy, non-covalent interaction (NCI), electron localization function (ELF), states (DOSs), electrostatic potential (ESP). Under pH, system showed stability a gap 3.22 eV, indicating low reactivity. In this decreased to 0.40 suggesting higher reactivity release. spectroscopy indicated weakened bonds conditions, length increasing from 1.288 Å 1.324 Å. NCI analysis revealed increased van der Waals interactions, supporting weakening. ELF at reactive sites, while DOS profiles ESP maps highlighted distinct electronic regions These findings confirm release triggered by tumor microenvironment.

Язык: Английский

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