Soil conditions and the plant microbiome boost the accumulation of monoterpenes in the fruit of Citrus reticulata ‘Chachi’

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Март 28, 2023

The medicinal material quality of Citrus reticulata 'Chachi' differs depending on the bioactive components influenced by planting area. Environmental factors, such as soil nutrients, plant-associated microbiome and climatic conditions, play important roles in accumulation citrus. However, how these environmental factors mediate production plants remains understudied.Here, a multi-omics approach was used to clarify role nutrients root-associated monoterpenes peel C. procured from core (geo-authentic product region) non-core (non-geo-authentic geographical regions. environment (high salinity, Mg, Mn K) enhanced monoterpene content promoting expression salt stress-responsive genes terpene backbone synthase host region. microbial effects citrus region were further verified synthetic community (SynCom) experiments. Rhizosphere microorganisms activated synthesis promoted through interactions with immune system. Endophyte derived potential for might enhance providing precursors monoterpenes.Overall, this study demonstrated that both properties impacted peel, thus an essential basis increasing fruit via reasonable fertilization precision microbiota management. Video Abstract.

Язык: Английский

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Geographical, climatic, and soil factors control the altitudinal pattern of rhizosphere microbial diversity and its driving effect on root zone soil multifunctionality in mountain ecosystems

The Science of The Total Environment, Год журнала: 2023, Номер 904, С. 166932 - 166932

Опубликована: Сен. 9, 2023

Shifts in rhizosphere soil microorganisms of dominant plants' response to climate change profoundly impact mountain ecosystem multifunctionality; relatively little is known about the relationship between them and how they depend on long-term environmental drivers. Here, we conducted analyses microbial altitudinal pattern, community assembly, co-occurrence network 6 plants six typical vegetation zones ranging from 1350 2900 m (a.s.l.) Helan Mountains by absolute quantitative sequencing technology, finally related microbiomes root zone multifunctionality ('soil multifunctionality' hereafter), dependence was explored. It found that pattern bacterial fungal diversities differed significantly. Higher more potential interactions Stipa breviflora Carex coninux were at lowest highest altitudes. Bacterial α diversity, identity some taxa, had significant positive or negative effects multifunctionality. The effect sizes diversity greater than those effects. These results indicated balance microbes determines As number phylum level increases, there will be a net gain Our study reveals geographical climatic factors can directly modulate properties thereby affecting driving multifunctionality, points rather fungi being strongly associated with This work has important ecological implications for predicting multiple environment-plant-soil-microorganisms ecosystems respond future change.

Язык: Английский

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Additive fungal interactions drive biocontrol of Fusarium wilt disease

New Phytologist, Год журнала: 2023, Номер 238(3), С. 1198 - 1214

Опубликована: Фев. 6, 2023

Summary Host‐associated fungi can help protect plants from pathogens, and empirical evidence suggests that such microorganisms be manipulated by introducing probiotic to increase disease suppression. However, we still generally lack the mechanistic knowledge of what determines success application, hampering development reliable suppression strategies. We conducted a three‐season consecutive microcosm experiment in which amended banana Fusarium wilt disease‐conducive soil with Trichoderma ‐amended biofertilizer or lacking this inoculum. High‐throughput sequencing was complemented cultivation‐based methods follow changes fungal microbiome explore potential links plant health. application increased biomass decreasing incidence up 72%, effect attributed microbiome, including reduction oxysporum density enrichment pathogen‐suppressing ( Humicola ). These were accompanied an expansion microbial carbon resource utilization potential, features contribute further demonstrated actions ‐ consortia, results suggest niche overlap pathogen induction systemic resistance may mechanisms driving observed biocontrol effects. Together, demonstrate inoculants modify composition functioning resident suppress soilborne disease.

Язык: Английский

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Vertically stratified methane, nitrogen and sulphur cycling and coupling mechanisms in mangrove sediment microbiomes

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Апрель 5, 2023

Abstract Background Mangrove ecosystems are considered as hot spots of biogeochemical cycling, yet the diversity, function and coupling mechanism microbially driven cycling along sediment depth mangrove wetlands remain elusive. Here we investigated vertical profile methane (CH 4 ), nitrogen (N) sulphur (S) genes/pathways their potential mechanisms using metagenome sequencing approaches. Results Our results showed that metabolic pathways involved in CH , N S were mainly shaped by pH acid volatile sulphide (AVS) a depth, AVS was critical electron donor impacting oxidation denitrification. Gene families denitrification significantly ( P < 0.05) decreased could be coupled S-driven denitrifiers, such Burkholderiaceae Sulfurifustis surface (0–15 cm). Interestingly, all denitrifier metagenome-assembled genomes (MAGs) appeared to incomplete denitrifiers with nitrate/nitrite/nitric oxide reductases (Nar/Nir/Nor) but without nitrous reductase (Nos), suggesting sulphide-utilizing groups might an important contributor 2 O production sediment. methanogenesis reduction increased depth. Based on both network MAG analyses, sulphate-reducing bacteria (SRB) develop syntrophic relationships anaerobic oxidizers (ANMEs) direct transfer or zero-valent sulphur, which would pull forward co-existence methanogens SRB middle deep layer sediments. Conclusions In addition offering perspective distribution genes/pathways, this study emphasizes role emissions various possible ANMEs The exploration provides novel insights into future synthetic microbial community construction analysis. This also has implications for predicting ecosystem functions within context environmental global change.

Язык: Английский

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Viral lysing can alleviate microbial nutrient limitations and accumulate recalcitrant dissolved organic matter components in soil

The ISME Journal, Год журнала: 2023, Номер 17(8), С. 1247 - 1256

Опубликована: Май 29, 2023

Язык: Английский

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Depth-dependent effects of tree species identity on soil microbial community characteristics and multifunctionality

The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162972 - 162972

Опубликована: Март 21, 2023

Язык: Английский

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Long-term (2003−2021) evolution trend of water quality in the Three Gorges Reservoir: An evaluation based on an enhanced water quality index

The Science of The Total Environment, Год журнала: 2024, Номер 915, С. 169819 - 169819

Опубликована: Янв. 6, 2024

Язык: Английский

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Soil microbial community and influencing factors of different vegetation restoration types in a typical agricultural pastoral ecotone

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 23, 2025

Microbial network complexity is an important indicator for assessing the effectiveness of vegetation restoration. However, response microbial bacteria and fungi to different restoration types unclear. Therefore, in this study, we selected four ( Pinus sylvestris var. mongholica , Larix principis- rupprechtii Populus tomentosa Ulmus pumila ), while nature grassland as a control, Zhangjiakou Tunken Forest Farm, which typical agricultural pastoral ecotone northern China, investigate soil diversity types. Our result showed that bacterial Shannon Chao indices P. were significantly 7.77 22.39% higher than those 20–40 cm layer, respectively. The fungal U. 85.70 146.86% 40–60 Compared natural grassland, networks became more complex plantation forests L. ). increased with carbon nitrogen. suitable planting Zhangjiakou, because its high carbon, nitrogen complexity. Bacterial community composition was found be closely related organic (SOC), total (TN), SOC, clay silt content. This improvement enhances ecological service function ecotone. These findings offer theoretical basis technical support ecologically fragile areas

Язык: Английский

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The diversity of microbial community and function varied in response to different agricultural residues composting

The Science of The Total Environment, Год журнала: 2020, Номер 715, С. 136983 - 136983

Опубликована: Янв. 27, 2020

Язык: Английский

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Long-term Fertilization Structures Bacterial and Archaeal Communities along Soil Depth Gradient in a Paddy Soil

Frontiers in Microbiology, Год журнала: 2017, Номер 8

Опубликована: Авг. 15, 2017

Soil microbes provide important ecosystem services. Though the effects of changes in nutrient availability due to fertilization on soil microbial communities topsoil (tilled layer, 0-20 cm) have been extensively explored, and their associations with nutrients subsoil (below 20 which is rarely impacted by tillage are still unclear. 16S rRNA gene amplicon sequencing was used investigate bacterial archaeal a Pup-Calric-Entisol treated for 32 years chemical fertilizer (CF) CF combined farmyard manure (CFM), reveal links between properties specific taxa both top- subsoil. The results showed that CFM treatments increased organic carbon (SOC), moisture (MO) total nitrogen (TN) while decreased nitrate_N content through profile. Fertilizer applications also Olsen phosphorus (OP) most layers. Microbial were significantly different from those Compared treatment, such as Nitrososphaera, Nitrospira, several members Acidobacteria Subdivision 3 genera incertae sedis, Leptolinea, Bellilinea substantially more abundant CFM. A co-occurrence based network analysis demonstrated SOC OP parameters positively correlated subsoil, respectively. Hydrogenophaga identified keystone genus topsoil, Phenylobacterium Steroidobacter above involved decomposition complex compounds carbon, nitrogen, transformations. This study revealed spatial variability long-term strongly shapes community composition interactions at high low taxonomic levels across whole

Язык: Английский

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