Lacticaseibacillus rhamnosus alleviates intestinal inflammation and promotes microbiota-mediated protection against Salmonella fatal infections

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Авг. 11, 2022

The fatal impairment of the intestinal mucosal barrier chicks caused by Salmonella significantly resulting economic losses in modern poultry industry. Probiotics are recognized for beneficially influencing host immune responses, promoting maintenance epithelial integrity, antagonistic activity against pathogenic microorganisms and health-promoting properties. Some basic studies attest to probiotic capabilities show that Lacticaseibacillus rhamnosus could protect mucosa from injury animals infected with Typhimurium. However, mechanisms underlying its protective effects still not fully understood. Here, we used chick infection model combined histological, immunological, molecular approaches address this question. results indicated L. reduced diarrhea rate increased daily weight gain survival S. Furthermore, found markedly improved immunity gut reducing apoptotic cells, hence effectively inhibiting inflammation. Notably, pre-treatment balanced expression interleukin-1β interleukin-18, moderated endotoxin D-lactic acid levels, expanded tight junction protein levels (Zonula occluden-1 Claudin-1), enhanced function cells. Additionally, investigations using full-length 16S rRNA sequencing also demonstrated greatly weakened adhesion , mainly manifestation is improvement diversity microbiota chicks. Collectively, these showed application enhancing integrity stability inflammation new hatch chicks, offering antibiotic alternatives farming animals.

Язык: Английский

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Quantitative microbiome profiling reveals the developmental trajectory of the chicken gut microbiota and its connection to host metabolism

iMeta, Год журнала: 2023, Номер 2(2)

Опубликована: Апрель 25, 2023

Abstract Revealing the assembly and succession of chicken gut microbiota is critical for a better understanding its role in physiology metabolism. However, few studies have examined dynamic changes absolute microbes using quantitative microbiome profiling (QMP) method. Here, we revealed developmental trajectory broiler bacteriome mycobiome by combining high‐throughput sequencing with microbial load quantification assay. We showed that abundance diversity reached plateau at 7 days posthatch (DPH), forming segment‐specific community types after 1 DPH. The was more impacted deterministic processes, affected stochastic processes. also observed stage‐specific different segments, three occurrence patterns including “colonization,” “disappearance,” “core” were defined. co‐occurrence networks very among positive associations than negative associations. Furthermore, provided links between their serum metabolite variations. Time‐course untargeted metabolomics six clusters changing abundance. foregut had connections metabolites, closely related to lipid amino acid present study full landscape development manner, metabolites further highlight impact growth development.

Язык: Английский

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Interaction between intestinal mycobiota and microbiota shapes lung inflammation

iMeta, Год журнала: 2024, Номер 3(5)

Опубликована: Сен. 14, 2024

Abstract Gut microbiota is an intricate microbial community containing bacteria, fungi, viruses, archaea, and protozoa, each of them contributes to diverse aspects host health. Nevertheless, the influence interaction among gut on health remains uncovered. Here, we showed that between intestinal fungi bacteria shaped lung inflammation during infection. Specifically, antifungal drug‐induced dysbiosis mycobiota enhanced Dysbiosis led Escherichia coli ( E. ) overgrowth translocation infection, which induced accumulation CD45 + F4/80 Ly6G − Ly6C CD11b CD11c macrophages. Clearance macrophages or deletion TLR4 (Toll‐like receptor 4, recognition LPS) rather than Dectin‐1 (recognition beta‐1,3/1,6 glucans fungi) blocked aggravation These findings suggest commensal affects through gut–lung axis, offering a potential therapeutic target for ameliorating

Язык: Английский

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Study on intestinal microbial communities of three different cattle populations on Qinghai-Tibet Plateau

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0312314 - e0312314

Опубликована: Фев. 4, 2025

The Tibetan cattle, indispensable·animals on the Qinghai-Tibet Plateau, have become a focal point for region’s economic development. As such, hybridization of these cattle has been recognized as pivotal strategy to enhance local industry. However, research gut microbiota hybrid remains scarce. Based this, we conducted comparative analysis and its functional implications across three distinct populations: two populations (Tibetan × Holstein TH Jersey TJ) one locoal population (BL). Bacteroidetes Firmicutes dominate all at phylum level. In addition, predominant phyla in BL were found be Cyanobacteria, Verrucomicrobiota, Actinobacteria, which may important reasons adaptability high-altitude environment Plateau. Further identified specific biomarkers associated with immune systems including Bacteroidales_RF16, Coriobacterium , Muribaculaceae. contrast, are primarily dominated by Oscillospiraceae Clostridia_UCG_014, TJ mainly Christensenellaceae Gammaproteobacteria. KEGG enrichment revealed that showed significant system, energy metabolism, amino acid metabolism-related pathways compared cattle. Overall, results suggest demonstrate enhanced indicate intestinal different altitudes breeds diverse structures functions. Our study presents new perspective role microbiome enhancement

Язык: Английский

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Yeasts and yeast-based products in poultry nutrition

The Journal of Applied Poultry Research, Год журнала: 2023, Номер 32(2), С. 100345 - 100345

Опубликована: Фев. 16, 2023

The poultry industry is interested in alternatives to antibiotics improve production performance and reduce the incidence of enteric diseases. For more than a decade, yeast fermentation products have been used feed growth efficiency livestock. cell wall comprised numerous polysaccharides: mannans, chitin, β-1,3-glucans, 1,6-glucans. These are major bioactive molecules that can impart health benefits host. Yeast modulate host immune response, load pathogens, ameliorate pathologic effects infections poultry. principal mechanisms action probiotics prebiotics competitive exclusion antagonism, immunomodulation, effect on digestive enzymes, direct nutritional benefits. However, only few species yeast, such as Saccharomyces thoroughly studied for their probiotic potential. Novel species, whole Pichia guilliermondii (Citrsitim) cells, recently shown prebiotic properties demand further investigation. A thorough understanding gut mycobiota required formulate tailor-made avian

Язык: Английский

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Carbohydrate-active enzymes in animal feed

Biotechnology Advances, Год журнала: 2023, Номер 65, С. 108145 - 108145

Опубликована: Апрель 7, 2023

Язык: Английский

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Description of Gut Mycobiota Composition and Diversity of Caprinae Animals

Microbiology Spectrum, Год журнала: 2023, Номер 11(1)

Опубликована: Янв. 10, 2023

The fungal community, also known as mycobiota, plays pivotal roles in host nutrition and metabolism has potential to cause disease. However, knowledge of the gut structure Caprinae is quite limited. In this study, composition diversity mycobiota animals from different geographical locations (Anhui, Jilin, Guangxi, Shandong, Shanxi, Tibet) were comprehensively characterized by analyzing internal transcribed spacer 2 (ITS-2) sequences community. results showed that Ascomycota Basidiomycota dominant phyla, which, respectively, accounted for 90.86 95.27% 2.58 7.62% samples each region. Nonetheless, was largely provinces. Therein, Sporormiaceae Thelebolaceae families Tibet, whereas their abundance generally low other regions. intestinal individuals Guangxi higher than addition, there 114 differential genera among all Finally, co-occurrence network revealed 285 significant correlations cross-family pairs guts animals, which contained 149 positive 136 negative relationships, with 96 bacterial 86 participants at family level. This study improved understanding ruminants provided support improvement animal health productivity. IMPORTANCE we elucidated analyzed differences environments. Based on previous findings, between communities analyzed. adds research expanded present microbiome animals.

Язык: Английский

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Supplementing Genistein for Breeder Hens Alters the Growth Performance and Intestinal Health of Offspring

Life, Год журнала: 2023, Номер 13(7), С. 1468 - 1468

Опубликована: Июнь 28, 2023

Recent research revealed that dietary genistein supplementation for breeder hens can improve the immune function of offspring chicks. However, it remains unknown whether this maternal effect could intestinal health offspring. This study was conducted to explore mechanism involved in on mucosa and microbial homeostasis chicken A total 120 Qiling were fed a basal diet, 20 mg/kg genistein-supplemented or 40 diet 4 weeks before collecting their eggs. After hatching, 180 male (60 chickens from each group) randomly selected divided into three groups: (1) (CON); (2) low-dose (LGE); (3) high-dose (HGE). At 17 d, 72 (48 CON 24 LGE) group challenged with LPS (LPS); LGE (LPS + LGE). The results showed increased birth weight serum level protein (TP), followed by improved villus morphology. Continuously, body chicks lasted until 21 d. Additionally, observed exhibited protective effects against LPS-induced morphological damage mucosal barrier dysfunction upregulating expression tight junction proteins, specifically ZO-1, Claudin1, E-cadherin, Occludin, at Using 16S rRNA gene sequencing, we demonstrated has potential facilitate maturation newly hatched enhancing abundance Escherichia coli. effectively reduce Gammaproteobacteria, thus mitigating risk bacterial diversity impairment LPS. In light these findings, holds promise as strategy ameliorating modulating microbiome

Язык: Английский

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Weaning transition, but not the administration of probiotic candidate Kazachstania slooffiae, shaped the gastrointestinal bacterial and fungal communities in nursery piglets

Frontiers in Veterinary Science, Год журнала: 2024, Номер 10

Опубликована: Янв. 11, 2024

As in-feed antibiotics are phased out of swine production, producers seeking alternatives to facilitate improvements in growth typically seen from this previously common feed additive.

Язык: Английский

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Temporal variability in the diversity, function and resistome landscapes in the gut microbiome of broilers

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 292, С. 117976 - 117976

Опубликована: Март 1, 2025

Understanding the dynamic and stability of gut microbiota over course production cycle broiler chicken can help identify microbial features that associate with better health productivity. In present study, we profile changes in composition commercially raised broilers at nine distinct time points using shotgun metagenomics culturomics approaches. We demonstrate, within first week post-hatching, a rapid decline relative abundance 122 pioneer species including Bacteroides fragilis, Lachnospira eligens Ruminococcus gnavus, accompanied by substantial decrease both richness diversity. This was followed gradual increase stabilization diversity population structure persisted until reached marketing age. Throughout cycle, key bacterial families such as Lachnospiraceae, Bacteroidaceae, Ruminococcaceae were identified. However, significant shifts lower taxonomic levels occurred different stages, influencing functional capacities resistance profiles microbiota. During growth phase, enzymes crucial to vitamin amino acid metabolism dominated, whereas associated carbohydrate energy notably more abundant during fattening stage. Many predicted antibiotic genes detected association typical commensal microbiota, indicating sustained classes aminoglycosides tetracyclines, which persist even absence selection pressure. Our research carries important implications for management surveillance production.

Язык: Английский

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