Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease DOI Creative Commons

Deng Guang-hui,

Chaofeng Wu, Yunjia Li

и другие.

Military Medical Research, Год журнала: 2023, Номер 10(1)

Опубликована: Ноя. 8, 2023

Abstract Background Nonalcoholic fatty liver disease (NAFLD) is associated with disordered lipid and iron metabolism. Our previous study has substantiated the pivotal role of Caveolin-1 (Cav-1) in protecting hepatocytes mediating metabolism liver. This aimed to explore specific mechanisms underlying regulation by Cav-1 NAFLD. Methods Hepatocyte-specific overexpression mice knockout were used this study. -knockdown RAW264.7 cells mouse primary performed verify changes vitro. Moreover, a high-fat diet palmitic acid plus oleic treatment utilized construct NAFLD model vivo vitro, respectively, while high-iron was an overload model. Besides, concentration, expression metabolism-related proteins tissue or serum detected using assay kit, Prussian blue staining, Western blotting, immunofluorescence immunohistochemical staining ELISA. The related indicators oxidative stress evaluated corresponding reagent kit staining. Results Significant disorder occurred decreased ( P < 0.05), accompanied disorder. enhanced storage capacity activating ferritin light chain/ferritin heavy chain pathway NAFLD, subsequently alleviating induced excess ferrous ions Further, CD68 + CD163 macrophages expressing found accelerate accumulation liver, which contrary effect hepatocytes. Positive correlations also observed between concentration iron-related protein levels patients healthy volunteers 0.05). Conclusions These findings confirm that essential target regulates metabolic homeostasis. It molecule for predicting against development

Язык: Английский

Research progress of therapeutic effects and drug resistance of immunotherapy based on PD-1/PD-L1 blockade DOI
Kun Pang,

Zhenduo Shi,

Liuya Wei

и другие.

Drug Resistance Updates, Год журнала: 2022, Номер 66, С. 100907 - 100907

Опубликована: Ноя. 30, 2022

Язык: Английский

Процитировано

113
Understanding the immunosuppressive microenvironment of glioma: mechanistic insights and clinical perspectives DOI Creative Commons

Hao Lin,

Chaxian Liu,

An-Kang Hu

и другие.

Journal of Hematology & Oncology, Год журнала: 2024, Номер 17(1)

Опубликована: Май 8, 2024

Abstract Glioblastoma (GBM), the predominant and primary malignant intracranial tumor, poses a formidable challenge due to its immunosuppressive microenvironment, thereby confounding conventional therapeutic interventions. Despite established treatment regimen comprising surgical intervention, radiotherapy, temozolomide administration, exploration of emerging modalities such as immunotherapy integration medicine engineering technology therapy, efficacy these approaches remains constrained, resulting in suboptimal prognostic outcomes. In recent years, intensive scrutiny inhibitory milieu within GBM has underscored significance cellular constituents microenvironment their interactions with cells neurons. Novel immune targeted therapy strategies have emerged, offering promising avenues for advancing treatment. One pivotal mechanism orchestrating immunosuppression involves aggregation myeloid-derived suppressor (MDSCs), glioma-associated macrophage/microglia (GAM), regulatory T (Tregs). Among these, MDSCs, though constituting minority (4–8%) CD45 + GBM, play central component fostering evasion propelling tumor progression, angiogenesis, invasion, metastasis. MDSCs deploy intricate mechanisms that adapt dynamic (TME). Understanding interplay between provides compelling basis This review seeks elucidate inherent explore existing targets, consolidate insights into MDSC induction contribution immunosuppression. Additionally, comprehensively surveys ongoing clinical trials potential strategies, envisioning future where targeting could reshape landscape GBM. Through synergistic other modalities, this approach can establish multidisciplinary, multi-target paradigm, ultimately improving prognosis quality life patients

Язык: Английский

Процитировано

59
Emerging nanosonosensitizers augment sonodynamic-mediated antimicrobial therapies DOI Creative Commons
Qinglin Xu, Weijun Xiu, Qiang Li

и другие.

Materials Today Bio, Год журнала: 2023, Номер 19, С. 100559 - 100559

Опубликована: Янв. 21, 2023

With the widespread prevalence of drug-resistant pathogens, traditional antibiotics have limited effectiveness and do not yield desired outcomes. Recently, alternative antibacterial therapies based on ultrasound (US) been explored to overcome crisis bacterial pathogens. Antimicrobial sonodynamic therapy (aSDT) offers an excellent solution that relies US irradiation produce reactive oxygen species (ROS) achieve antibiotic-free mediated antimicrobial effects. In addition, aSDT possesses advantage superior tissue penetrability compared light irradiation, demonstrating great feasibility in treating deep infections. Although existing conventional sonosensitizers can ROS for activity, some limitations, such as low penetration rate, nonspecific distribution poor production under hypoxic conditions, result suboptimal sterilization aSDT. emerging nanosonosensitizers enormous advantages high-performance agents aSDT, which deficiencies described above. Thus, nanosonosensitizer-mediated has a bright future management This review classifies current available provides overview mechanisms, biomedical applications, recent advances perspectives

Язык: Английский

Процитировано

50
Applications of Focused Ultrasound for the Treatment of Glioblastoma: A New Frontier DOI Open Access
Andrew M. Hersh, Meghana Bhimreddy, Carly Weber-Levine

и другие.

Cancers, Год журнала: 2022, Номер 14(19), С. 4920 - 4920

Опубликована: Окт. 8, 2022

Glioblastoma (GBM) is an aggressive primary astrocytoma associated with short overall survival. Treatment for GBM primarily consists of maximal safe surgical resection, radiation therapy, and chemotherapy using temozolomide. Nonetheless, recurrence tumor progression the norm, driven by stem cell activity a high mutational burden. Focused ultrasound (FUS) has shown promising results in preclinical clinical trials treatment received regulatory approval other neoplasms. Here, we review range applications FUS GBM, which depend on parameters, including frequency, power, pulse duration, duty cycle. Low-intensity can be used to transiently open blood–brain barrier (BBB), restricts diffusion most macromolecules therapeutic agents into brain. Under guidance from magnetic resonance imaging, BBB targeted precise location permit molecules only at vicinity tumor, preventing side effects healthy tissue. opening also improve detection cell-free DNA liquid biopsies, allowing non-invasive diagnosis identification molecular mutations. High-intensity cause ablation via hyperthermic effect. Additionally, stimulate immunological attack cells, activate sonosensitizers exert cytotoxic tissue, sensitize tumors therapy. Finally, another mechanism under investigation, known as histotripsy, produces acoustic cavitation rather than thermal effects.

Язык: Английский

Процитировано

46
Platelet membrane-coated C-TiO2 hollow nanospheres for combined sonodynamic and alkyl-radical cancer therapy DOI

Weihong Guo,

Tao Wang, Chunyu Huang

и другие.

Nano Research, Год журнала: 2022, Номер 16(1), С. 782 - 791

Опубликована: Июль 28, 2022

Язык: Английский

Процитировано

42
Sonodynamic therapy-based nanoplatforms for combating bacterial infections DOI Creative Commons

Pei‐Yao Xu,

Ranjith Kumar Kankala, Shi‐Bin Wang

и другие.

Ultrasonics Sonochemistry, Год журнала: 2023, Номер 100, С. 106617 - 106617

Опубликована: Сен. 23, 2023

The rapid spread and uncontrollable evolution of antibiotic-resistant bacteria have already become urgent global to treat bacterial infections. Sonodynamic therapy (SDT), a noninvasive effective therapeutic strategy, has broadened the way toward dealing with biofilms, which base on ultrasound (US) sonosensitizer. Sonosensitizer, based small organic molecules or inorganic nanoparticles, is essential SDT process. Thus, it meaningful design sonosensitizer-loaded nanoplatform synthesize an efficient effect. In this review, we initially summarize probable SDT-based antibacterial mechanisms systematically discuss current advancement in different (including for small-molecule sonosensitizer delivery as sonosensitizer) infection therapy. addition, biomedical applications SDT-involved multifunctional nanoplatforms are also discussed. We believe innovative would highly next-generation tool combating infection.

Язык: Английский

Процитировано

33
Mapping knowledge landscapes and emerging trends of sonodynamic therapy: A bibliometric and visualized study DOI Creative Commons

Zhenjiang Wu,

Kunming Cheng,

Zefeng Shen

и другие.

Frontiers in Pharmacology, Год журнала: 2023, Номер 13

Опубликована: Янв. 9, 2023

Background: Ultrasound-triggered sonodynamic therapy (SDT), as a non-invasive approach, has attracted considerable attention in wide variety of malignant tumors and other diseases. Over the past 2 decades, number scientific publications on SDT increased rapidly. However, there is still lack one comprehensive report that summarizes global research trends knowledge landscapes field detail. Thus, we performed bibliometric analysis from 2000 to 2021 track current hotspots highlight future directions. Methods: We collected Web Science Core Collection database. The annual citations, major contributors, popular journals, international collaborations, co-cited references co-occurrence keywords were analyzed visualized with CiteSpace, VOSviewer, R-bibliometrix. Results: A total 701 included. publication output 5 175 2021, average growth rate was 18.4%. China most productive country 463 documents (66.05%), Harbin Medical University prolific institution (N = 73). Ultrasound Medicine Biology published papers related SDT. Materials , Chemistry areas receiving interest. All divided into four different clusters including studies mechanisms, drug delivery nanoparticles, cancer therapy, well ultrasound sonosensitizers. In addition nanomaterials-related mesoporous silica nanosheets, liposomes, microbubble TiO nanoparticle, following directions such immunogenic cell death, metal-organic framework, photothermal hypoxia, tumor microenvironment, chemodynamic combination resistance, intensity focused ultrasound, delivery, Staphylococcus aureus also deserve further may continue explode future. Conclusion: bright treatment, nanomaterials have increasingly influenced development nano-technology. Overall, this study first attempt analyze SDT, which could provide valuable for later researchers better understand trends, frontiers domain.

Язык: Английский

Процитировано

29
Elimination of methicillin-resistant Staphylococcus aureus biofilms on titanium implants via photothermally-triggered nitric oxide and immunotherapy for enhanced osseointegration DOI Creative Commons
Yonglin Yu,

Junjie Wu,

Chuanchuan Lin

и другие.

Military Medical Research, Год журнала: 2023, Номер 10(1)

Опубликована: Май 4, 2023

Treatment of methicillin-resistant Staphylococcus aureus (MRSA) biofilm infections in implant placement surgery is limited by the lack antimicrobial activity titanium (Ti) implants. There a need to explore more effective approaches for treatment MRSA infections.Herein, an interfacial functionalization strategy proposed integration mesoporous polydopamine nanoparticles (PDA), nitric oxide (NO) release donor sodium nitroprusside (SNP) and osteogenic growth peptide (OGP) onto Ti implants, denoted as Ti-PDA@SNP-OGP. The physical chemical properties Ti-PDA@SNP-OGP were assessed scanning electron microscopy, X-ray photoelectron spectroscope, water contact angle, photothermal property NO behavior. synergistic antibacterial effect elimination biofilms evaluated 2',7'-dichlorofluorescein diacetate probe, 1-N-phenylnaphthylamine assay, adenosine triphosphate intensity, o-nitrophenyl-β-D-galactopyranoside hydrolysis activity, bicinchoninic acid leakage. Fluorescence staining, assays alkaline phosphatase collagen secretion extracellular matrix mineralization, quantitative real‑time reverse transcription‑polymerase chain reaction, enzyme-linked immunosorbent assay (ELISA) used evaluate inflammatory response ability bone marrow stromal cells (MSCs), RAW264.7 their co-culture system. Giemsa ELISA, micro-CT, hematoxylin eosin, Masson's trichrome immunohistochemistry staining eradication biofilms, inhibition response, promotion osseointegration vivo.Ti-PDA@SNP-OGP displayed NO-dependent against following near-infrared light irradiation, effectively eliminated formed inducing reactive oxygen species (ROS)-mediated oxidative stress, destroying bacterial membrane integrity causing leakage intracellular components (P < 0.01). In vitro experiments revealed that not only facilitated differentiation MSCs, but also promoted polarization pro-inflammatory M1 macrophages anti-inflammatory M2-phenotype 0.05 or P favorable osteo-immune microenvironment further osteogenesis MSCs anti-inflammation via multiple paracrine signaling pathways vivo evaluation confirmed aforementioned results induced ameliorative MRSA-infected femoral defect implantation model 0.01).These findings suggest promising multi-functional material high-efficient replacement surgeries.

Язык: Английский

Процитировано

29
Edible plant-derived extracellular vesicles serve as promising therapeutic systems DOI Creative Commons
Chunhua Yang, Wenjing Zhang,

Muran Bai

и другие.

Nano TransMed, Год журнала: 2023, Номер 2(2-3), С. 100004 - 100004

Опубликована: Сен. 1, 2023

Extracellular vesicles (EVs), which are natural nanocarriers characterized by a phospholipid bilayer structure, released living cells. They play crucial role in the intercellular transport of proteins, nucleic acids, lipids, and metabolites, facilitating substance delivery information exchange between In light recent numerous studies, EVs has been found to transcend their basic as mere vehicle. Instead, they demonstrate an impressive array biological activities, displaying preventive therapeutic potential mitigating various pathological processes encompassing cancer, neoplastic proliferation, infectious diseases, oxidative trauma. Particularly, derived from edible plants (EPDEVs) have emphasized for extensive range physiological regulatory functions animals humans, with targeted drug through oral administration. Leveraging these advantages, EPDEVs expected excellent competitiveness clinical applications or healthcare products. This review provides brief overview biogenesis, composition EPDEVs, summarizes mechanisms. It also analyzes methods isolating purifying plant-EVs, assessing advantages disadvantages; discusses latest advancements biomedical applications, concludes prospective insight into research development directions EPDEVs.

Язык: Английский

Процитировано

25
Multiple therapeutic approaches of glioblastoma multiforme: From terminal to therapy DOI
Smita Kumari,

Rohan Gupta,

Rashmi K. Ambasta

и другие.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2023, Номер 1878(4), С. 188913 - 188913

Опубликована: Май 12, 2023

Язык: Английский

Процитировано

24