Cancer Letters, Год журнала: 2024, Номер unknown, С. 217397 - 217397
Опубликована: Дек. 1, 2024
Язык: Английский
CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(5)
Опубликована: Май 1, 2024
Abstract Gliomas are the most common primary tumors of central nervous system, with glioblastoma multiforme (GBM) having highest incidence, and their therapeutic efficacy depends primarily on extent surgical resection postoperative chemotherapy. The role intracranial blood–brain barrier occurrence drug‐resistant gene O6‐methylguanine‐DNA methyltransferase have greatly limited chemotherapeutic agents in patients GBM made it difficult to achieve expected clinical response. In recent years, rapid development nanotechnology has brought new hope for treatment tumors. Nanoparticles (NPs) shown great potential tumor therapy due unique properties such as light, heat, electromagnetic effects, passive targeting. Furthermore, NPs can effectively load drugs, significantly reduce side effects improve efficacy, showing chemotherapy glioma. this article, we reviewed mechanisms glioma drug resistance, physicochemical NPs, advances resistance. We aimed provide perspectives
Язык: Английский
Процитировано
11
Cell Death and Disease, Год журнала: 2024, Номер 15(10)
Опубликована: Окт. 5, 2024
Язык: Английский
Процитировано
5
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 7, 2025
Glioblastoma multiforme (GBM) is the most common and aggressive primary intracranial malignancy. It characterized by insufficient infiltration of anti-tumor T lymphocytes within tumor microenvironment (TME), rendering it an "immune cold" disease. This immune deficiency results in poor responses to checkpoint blockade (ICB) therapies. Recent studies have demonstrated that bacteria can proliferate tumors activate responses. Therefore, this study, we employed Escherichia coli (E. coli) combination with anti-PD-1 antibodies treat GBM, aim exploring immune-activating potential E. GBM its synergistic effect on therapy. The antibody therapy were administered intravenously intraperitoneally, respectively. Complete blood cell count, biochemical analysis, hematoxylin eosin (H&E) staining, agar plate culture evaluate biosafety tumor-targeting capability coli. ELISA kits used detect innate cytokines. Flow cytometry immunofluorescence staining investigate cells. Tumor volume tumor-bearing mice was recorded combined treatment efficacy. H&E observe inhibition markers. E.coli specifically target into region, response mice. Immunofluorescence flow group exhibited a significant upregulation cytotoxic CD8+ cells marked suppression regulatory compared control group. expression Ki67 significantly downregulated, TUNEL revealed increased number apoptotic Furthermore, growth rate slower than exhibits activity further regulate tissues synergize providing new insights enhance efficacy immunotherapy.
Язык: Английский
Процитировано
0
Cancer Science, Год журнала: 2025, Номер unknown
Опубликована: Март 7, 2025
Neutrophils, as key components of the tumor microenvironment, play a crucial role in cancer progression and prognosis. This study aimed to identify neutrophil-related gene signature improve prognostic predictions explore potential immunotherapy targets for glioblastoma multiforme (GBM) patients. Through co-expression analysis, 60 neutrophil-associated genes were identified, showing significant enrichment 159 Gene Ontology terms eight KEGG pathways. Among these, 10 significantly associated with patient survival, leading development six-gene risk model termed Neutrophil-Related Prognostic Index (NRGPI). The NRGPI predicted overall survival (OS) both training validation cohorts (p < 0.05), enhanced accuracy when combined clinicopathological factors. Higher scores correlated worse OS, increased mortality, more aggressive disease progression. Immune profiling linked immune cell infiltration, checkpoint expression, mutation burden, suggesting its identifying candidates immunotherapy. identified genes, FN1 emerged central regulator, composition poor Pan-cancer analysis revealed consistent upregulation across types, underscoring broad clinical relevance. Additionally, tissue microarray using multiplex immunofluorescence on 84 GBM samples confirmed FN1, SDC1, TWIST1, higher levels reduced survival. These findings establish valuable biomarker GBM, offering novel insights into landscape positioning promising therapeutic target further investigation treatment.
Язык: Английский
Процитировано
0
Advanced Materials, Год журнала: 2023, Номер 36(15)
Опубликована: Окт. 9, 2023
Messenger RNA (mRNA)-based therapeutic strategies have shown remarkable promise in preventing and treating a staggering range of diseases. Optimizing the structure delivery system engineered mRNA has greatly improved its stability, immunogenicity, protein expression levels, which led to wider uses for therapeutics. Herein, thorough analysis optimization used is first provided systems are described great detail. Furthermore, latest advancements biomedical engineering technology, including applications combatting infectious diseases, cancer, providing replacement therapy, conducting gene editing, more, summarized. Lastly, perspective on forthcoming challenges prospects concerning advancement therapeutics offered. Despite these challenges, mRNA-based remain promising, with potential revolutionize disease treatment contribute significant field.
Язык: Английский
Процитировано
12
Journal of Neuro-Oncology, Год журнала: 2024, Номер 170(2), С. 235 - 252
Опубликована: Авг. 29, 2024
Abstract Purpose Brain tumors, particularly glioblastoma multiforme (GBM), present significant prognostic challenges despite multimodal therapies, including surgical resection, chemotherapy, and radiotherapy. One major obstacle is the limited drug delivery across blood–brain barrier (BBB). Focused ultrasound (FUS) combined with systemically administered microbubbles has emerged as a non-invasive, targeted, reversible approach to transiently open BBB, thus enhancing delivery. This review examines clinical trials employing BBB opening techniques optimise pharmacotherapy for brain evaluates current challenges, proposes directions further research. Methods A systematic literature search was conducted in PubMed ClinicalTrials.gov up November 2023, searching “ultrasound” AND “brain tumor”. The yielded 1446 results. After screening by title abstract, followed full-text (n = 48), 35 studies were included analysis. Results Our analysis includes data from 11 published 24 ongoing trials. predominant focus of these on glioma, GMB astrocytoma. paper investigated metastasis breast cancer. Evidence indicates that FUS facilitates enhances uptake following sonication. Exploration pediatric population limited, no only three dedicated this demographic. Conclusion promising strategy safely disrupt enabling precise non-invasive lesion targeting, enhance However, pharmacokinetic are required quantitatively assess improvements uptake. Most phase I trials, long-term follow-up investigating patient outcomes essential evaluate benefit treatment approach. Further involving diverse populations pathologies will be beneficial.
Язык: Английский
Процитировано
4
IntechOpen eBooks, Год журнала: 2025, Номер unknown
Опубликована: Янв. 23, 2025
The COVID-19 pandemic significantly disrupted glioblastoma (GBM) management, affecting its pathophysiology, diagnosis, treatment, and prognosis. GBM, an aggressive brain tumor with poor survival rates, presented unique vulnerabilities during the due to rapid progression patients’ compromised immunity. SARS-CoV-2 may also influence GBM by binding overexpressed receptors on cells, potentially activating oncogenic pathways. This interaction highlights potential for exacerbate aggressiveness. Diagnostic delays resulted in increased volumes, while treatment pathways adapted minimally invasive surgeries, hypofractionated radiotherapy, telemedicine ensure continuity of care. Despite these innovations, outcomes varied globally, centers adopting flexible protocols reporting fewer adverse impacts. Vaccination emerged as a critical tool, prioritization patients minimal effects, providing protection against severe COVID-19. paper explores multifaceted impact detailing disruptions strategies that paved way resilient neuro-oncological care after pandemic.
Язык: Английский
Процитировано
0
ChemistrySelect, Год журнала: 2025, Номер 10(4)
Опубликована: Янв. 1, 2025
Abstract Therapeutic trends involve designing ligands to target proteins in various diseases, but no parallel filters have been established prioritize pathological within pathogens for optimal inhibition. This study unveils that order a protein serve as an therapeutic intervention, it must exhibit five key attributes. It should druggable site with recognized cleft which ligand can be fitted high specificity. possess unique structure, distinct sequences having low similarity other non‐targeted proteins. dispensable the pathogenesis of disease, and case microbial infection, crucial survival infectious organisms. available crystallized 3D structure enable high‐throughput screening candidate ligands. occupy accessible localization provide easy route reach its less effort. The justifies rules essential considering ideal any protein‐mediated dysfunction. When these targeting are found protein, eradicating intended ailment primarily enhanced time, money,
Язык: Английский
Процитировано
0
Molecular Pharmaceutics, Год журнала: 2025, Номер unknown
Опубликована: Март 16, 2025
A nanoplatform based on self-assembling peptides was developed with the ability to effectively transport and deliver a wide range of moieties across blood–brain barrier (BBB) for treatment glioblastoma. Its surface functionalized have targeted release TMZ thanks targeting peptide that binds EGFRvIII, which is overexpressed tumor cells, gH625, acts as an enhancer penetration. Furthermore, on-demand achieved through matrix metalloproteinase-9 (MMP-9) cleavage. Nanofibers were characterized their stability, critical aggregation concentration, morphology. Next, effect both 2D 3D glioblastoma/astrocytoma (U-87) glioma (U-118) cell lines evaluated. The Annexin V/Propidium iodide showed increase in necrotic apoptotic morphological analysis allowed discover U-118 U-87 spheroids are smaller surface, perimeter, Feret's diameter when treated NF-TMZ. nanofiber demonstrated permeate BBB vitro spheroidal biodynamic model. Finally, there no cytotoxic effects nanofibers without drug spheroids, while significant decrease viability observed NF-TMZ used. Overall, these results open new opportunities evaluation efficacy safety this vivo studies.
Язык: Английский
Процитировано
0
Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)
Опубликована: Май 9, 2024
Abstract Background LncRNAs regulate tumorigenesis and development in a variety of cancers. We substantiate for the first time that LINC00606 is considerably expressed glioblastoma (GBM) patient specimens linked with adverse prognosis. This suggests may have potential to glioma genesis progression, biological functions molecular mechanisms GBM remain largely unknown. Methods The expression ATP11B normal brain tissues was evaluated by qPCR, LINC00606/miR-486-3p/TCF12/ATP11B axis were verified through series vitro vivo experiments. mechanism elucidated immunoblotting, FISH, RNA pulldown, CHIP-qPCR, dual-luciferase reporter assay. Results demonstrated promotes cell proliferation, clonal expansion migration, while reducing apoptosis levels. Mechanistically, on one hand, can sponge miR-486-3p; target gene TCF12 miR-486-3p affects transcriptional initiation LINC00606, PTEN KLLN. On other it also PI3K/AKT signaling pathway mediate migration binding protein. Conclusions Overall, involved regulation progression plays role tumor at post-transcriptional levels primarily sponging targeted ATP11B. Therefore, our research regulatory network could be novel therapeutic strategy treatment GBM. Graphical highly patients carcinogenic function correlated poor regulates interacting
Язык: Английский
Процитировано
3