Heliyon, Год журнала: 2024, Номер 10(19), С. e38660 - e38660
Опубликована: Сен. 27, 2024
Язык: Английский
Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 92, С. 105338 - 105338
Опубликована: Янв. 6, 2024
The rapid advancement of CRISPR-Cas9 technology has instigated a profound transformation in genome editing with significant implications for fields like health, agriculture, and biotechnology. This abstract provides an overview the historical significance fundamental components CRISPR-Cas9, notably Cas9 protein guide RNA, underscoring its pivotal role genetic manipulation. It emphasizes CRISPR-Cas9's preeminence domain precise editing, driving breakthroughs personalized medicine, gene therapy, agriculture. Of paramount importance is integration nanomaterials, encompassing lipid-based polymeric nanoparticles, alongside viral vectors, serving as potent vehicles augmenting delivery efficiency precision. We explore strategies aimed at enhancing through while also addressing ethical regulatory considerations. In expert opinion section, we offer nuanced perspective on present state field, highlighting potential transformative progress research therapy. stands brink unlocking new possibilities providing innovative solutions to address pressing global challenges.
Язык: Английский
Процитировано
30
Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)
Опубликована: Ноя. 26, 2024
Epigenetics governs a chromatin state regulatory system through five key mechanisms: DNA modification, histone RNA remodeling, and non-coding regulation. These mechanisms their associated enzymes convey genetic information independently of base sequences, playing essential roles in organismal development homeostasis. Conversely, disruptions epigenetic landscapes critically influence the pathogenesis various human diseases. This understanding has laid robust theoretical groundwork for developing drugs that target epigenetics-modifying pathological conditions. Over past two decades, growing array small molecule targeting such as methyltransferase, deacetylase, isocitrate dehydrogenase, enhancer zeste homolog 2, have been thoroughly investigated implemented therapeutic options, particularly oncology. Additionally, numerous epigenetics-targeted are undergoing clinical trials, offering promising prospects benefits. review delineates epigenetics physiological contexts underscores pioneering studies on discovery implementation drugs. include inhibitors, agonists, degraders, multitarget agents, aiming to identify practical challenges avenues future research. Ultimately, this aims deepen epigenetics-oriented strategies further application settings.
Язык: Английский
Процитировано
20
Cytokine & Growth Factor Reviews, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
2
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12
Опубликована: Янв. 18, 2024
Gene therapy is a technique that rectifies defective or abnormal genes by introducing exogenous into target cells to cure the disease. Although gene has gained some accomplishment for diagnosis and of inherited acquired cardiovascular diseases, how efficiently specifically deliver targeted lesion sites without being cleared blood system remains challenging. Based on nanotechnology development, non-viral vectors provide promising strategy overcoming difficulties in therapy. At present, according physicochemical properties, nanotechnology-based include polymers, liposomes, lipid nanoparticles, inorganic nanoparticles. Non-viral have an advantage safety, efficiency, easy production, possessing potential clinical application value when compared with viral vectors. Therefore, we summarized recent research progress diseases based commonly used vectors, hopefully providing guidance orientation future relevant research.
Язык: Английский
Процитировано
8
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(2), С. 114 - 114
Опубликована: Фев. 10, 2025
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a prevalent inflammatory disease of the upper airway, contributing significantly to global burden. CRSwNP characterized by sustained and exaggerated inflammation, accompanied marked changes in gene protein expression regulated through intricate molecular mechanisms. MicroRNAs (miRNAs), small single-stranded RNA molecules that regulate at transcriptional post-transcriptional levels, have emerged as pivotal players pathophysiology. Dysregulated miRNA implicated numerous human diseases, including cancer, asthma, disorders, highlighting their broad clinical relevance. In CRSwNP, miRNAs influence important pathways, T2 immune responses epithelial-mesenchymal transition (EMT), which leads chronic inflammation tissue remodeling. Profiling studies identified specific potential biomarkers for severity, prognosis, therapeutic response, offering pathway personalized medicine. Furthermore, advances extracellular vesicles (sEVs) exosomes, naturally transport miRNAs, provide innovative avenues targeted delivery, minimizing systemic side effects. This review explores current knowledge on function emphasizing role pathogenesis targets.
Язык: Английский
Процитировано
1
Cancers, Год журнала: 2025, Номер 17(4), С. 579 - 579
Опубликована: Фев. 8, 2025
We are on the brink of a paradigm shift in both theoretical and clinical oncology. Genomic transcriptomic profiling, alongside personalized approaches that account for individual patient variability, increasingly shaping discourse. Discussions future cancer medicine mainly dominated by potential non-coding RNAs (ncRNAs), which play prominent role progression metastasis formation regulating expression oncogenic or tumor suppressor proteins at transcriptional post-transcriptional levels; furthermore, their cell-free counterparts might be involved intercellular communication. Non-coding considered to promising biomarker candidates early diagnosis as well therapeutic agents. This review aims provide clarity amidst vast body literature focusing diverse species ncRNAs, exploring structure, origin, function, applications miRNAs, siRNAs, lncRNAs, circRNAs, snRNAs, snoRNAs, eRNAs, paRNAs, YRNAs, vtRNAs, piRNAs. discuss molecular methods used detection functional studies vitro vivo. also address challenges must overcome enter new era therapy will reshape
Язык: Английский
Процитировано
1
Pathology - Research and Practice, Год журнала: 2025, Номер unknown, С. 155956 - 155956
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)
Опубликована: Июнь 14, 2024
Abstract Respiratory disorders are among the conditions that affect respiratory system. The healthcare sector faces challenges due to emergence of drug resistance prescribed medications for these illnesses. However, there is a technology called CRISPR/Cas9, which uses RNA guide DNA targeting. This has revolutionized our ability manipulate and visualize genome, leading advancements in research treatment development. It can effectively reverse epigenetic alterations contribute resistance. Some studies focused on health have shown targeting genes using CRISPR/Cas9 be challenging when it comes reducing patients with disorders. Nevertheless, important acknowledge limitations this technology, such as off-target effects, immune system reactions Cas9, associated delivery methods. Despite limitations, review aims provide knowledge about genome editing tools explore how they help overcome Additionally, study discusses concerns related applications CRISPR provides an overview successful clinical trial studies.
Язык: Английский
Процитировано
4
Neuroscience, Год журнала: 2024, Номер 553, С. 1 - 18
Опубликована: Июнь 27, 2024
Язык: Английский
Процитировано
4
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Сен. 27, 2024
Язык: Английский
Процитировано
4