Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

и другие.

Опубликована: Ноя. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Язык: Английский

Neural mechanism of circadian clock-based photoperiodism in insects and snails DOI Creative Commons
Yoshitaka Hamanaka, Masaharu Hasebe, Sakiko Shiga

и другие.

Journal of Comparative Physiology A, Год журнала: 2023, Номер 210(4), С. 601 - 625

Опубликована: Авг. 18, 2023

Abstract The photoperiodic mechanism distinguishes between long and short days, the circadian clock system is involved in this process. Although necessity of genes for responses has been demonstrated many species, how contributes to mechanisms remains unclear. A comprehensive study, including functional analysis relevant physiology their expressing cells, necessary understand molecular cellular mechanisms. Since Drosophila melanogaster exhibits a shallow photoperiodism, have studied non-model starting with brain microsurgery neuroanatomy, followed by genetic manipulation some insects. Here, we review discuss involvement terms neural networks We also recent advances underlying insects snails, additionally systems whose photoperiodism hardly addressed yet. Brain neurosecretory insulin-like peptide / diuretic hormone44- pars intercerebralis neurones bean bug Riptortus pedestris caudo-dorsal cell hormone -expressing cells snail Lymnaea stagnalis , both promote egg laying under electrical excitability attenuated medium which reduces oviposition. are mediated glutamate control gene period . Thus, now able assess response activity investigate upstream mechanisms, that is, counter.

Язык: Английский

Процитировано

9
Drosophila ezoana uses morning and evening oscillators to adjust its rhythmic activity to different daylengths but only the morning oscillator to measure night length for photoperiodic responses DOI Creative Commons
Koustubh M. Vaze, Giulia Manoli, Charlotte Helfrich‐Förster

и другие.

Journal of Comparative Physiology A, Год журнала: 2023, Номер 210(4), С. 535 - 548

Опубликована: Июнь 17, 2023

Abstract Animals living at high latitudes are exposed to prominent seasonal changes which they need adapt survive. By applying Zeitgeber cycles of different periods and photoperiods we show here that high-latitude D. ezoana flies possess evening oscillators highly damped morning help them adapting their activity rhythms long photoperiods. In addition, the involved in timing diapause. The measure night length use external coincidence for We discuss clock protein TIMELESS (d-TIM) as molecular correlate small ventrolateral neurons (s-LN v s) anatomical correlates components measuring length.

Язык: Английский

Процитировано

8
Neuropeptide expression and action in the reproductive system of the starfish Asterias rubens DOI Creative Commons
Víctor M González,

Yuling Feng,

Michaela Egertová

и другие.

The Journal of Comparative Neurology, Год журнала: 2024, Номер 532(1)

Опубликована: Янв. 1, 2024

Abstract Reproductive processes are regulated by a variety of neuropeptides in vertebrates and invertebrates. In starfish (phylum Echinodermata), relaxin‐like gonad‐stimulating peptide triggers oocyte maturation spawning. However, little is known about other as potential regulators reproduction starfish. To address this issue, here, we used histology immunohistochemistry to analyze the reproductive system Asterias rubens at four stages seasonal cycle male female animals, investigating expression eight neuropeptides: corticotropin‐releasing hormone‐type neuropeptide ArCRH, calcitonin‐type ArCT, pedal peptide‐type ArPPLN1b ArPPLN2h, vasopressin/ocytocin‐type asterotocin, gonadotropin‐releasing ArGnRH, somatostatin/allatostatin‐C‐type ArSS1 ArSS2. The five neuropeptides, ArPPLN1b, was detected gonoducts and/or gonads. For example, extensive ArPPLN2h revealed coelomic epithelial layer gonads throughout both males females. sexual differences patterns were also observed. Informed these findings, vitro pharmacological effects on gonad preparations from investigated. This that causes gonadal contraction relaxation, with being more effective ovaries than testes. Collectively, findings indicate multiple signaling systems involved regulation function starfish, some exerting excitatory or inhibitory contractility may be physiologically relevant when gametes expelled during

Язык: Английский

Процитировано

2
Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis inDrosophila DOI Creative Commons
Jayati Gera, Marishia Agard,

Hannah Nave

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 10, 2024

Insects have evolved a variety of neurohormones that enable them to maintain their nutrient and osmotic homeostasis. While the identities functions various insect metabolic diuretic hormones been well-established, characterization an anti-diuretic signaling system is conserved across most insects still lacking. To address this, here we characterized ion transport peptide (ITP) in Drosophila . The ITP gene encodes five transcript variants which generate three different isoforms: amidated (ITPa) two ITP-like (ITPL1 ITPL2) isoforms. Using combination anatomical mapping single-cell transcriptome analyses, comprehensively expression all isoforms nervous peripheral tissues. Our analyses reveal widespread Moreover, show ITPa released during dehydration recombinant inhibits peptide-induced renal tubule secretion ex vivo , thus confirming its role as hormone. phylogenetic-driven approach assay, identified functionally Gyc76C, membrane guanylate cyclase, elusive receptor. Thus, knockdown Gyc76C tubules abolishes inhibitory effect on hormone secretion. Extensive reveals it highly expressed larval adult tissues associated with osmoregulation (renal rectum) homeostasis (fat body). Consistent this expression, impacts tolerance ionic stresses, whereas specifically fat body feeding, behaviors. We also complement receptor experiments overexpression neurons. Interestingly, ITPa-Gyc76C pathways deciphered are reminiscent atrial natriuretic mammals. Lastly, utilized connectomics transcriptomics identify synaptic paracrine upstream downstream ITP-expressing Taken together, our systematic establishes tractable decipher how small set neurons integrates diverse inputs orchestrate systemic

Язык: Английский

Процитировано

2
Neuropeptide-dependent spike time precision and plasticity in circadian output neurons DOI Creative Commons
Bryan Chong, Vipin Kumar, Dieu Linh Nguyen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 7, 2024

Abstract Circadian rhythms influence various physiological and behavioral processes such as sleep-wake cycles, hormone secretion, metabolism. output neurons are a group of that receive input from the central circadian clock located in suprachiasmatic nucleus mammalian brain transmit timing information to different regions body, coordinating processes. In Drosophila , an important set called pars intercerebralis (PI) neurons, which specific DN1. These can further be subdivided into functionally anatomically distinctive anterior (DN1a) posterior (DN1p) clusters. The neuropeptide diuretic hormones 31 (Dh31) 44 (Dh44) insect neuropeptides known activate PI control activity rhythms. However, neurophysiological basis how Dh31 Dh44 affect neural coding mechanisms underlying sleep is not well understood. Here, we identify Dh31/Dh44-dependent spike time precision plasticity neurons. We find application synthesized affects membrane potential dynamics precise neuronal firing through their synergistic interaction, possibly mediated by calcium-activated potassium channel conductance. Further, characterize Dh31/Dh44 enhances postsynaptic potentials Together, these results suggest multiplexed neuropeptide-dependent .

Язык: Английский

Процитировано

2
Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

и другие.

Опубликована: Ноя. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Язык: Английский

Процитировано

2
Characterization of clock‐related proteins and neuropeptides in Drosophila littoralis and their putative role in diapause DOI Creative Commons
Giulia Manoli, Meet Zandawala, Taishi Yoshii

и другие.

The Journal of Comparative Neurology, Год журнала: 2023, Номер 531(15), С. 1525 - 1549

Опубликована: Июль 26, 2023

Insects from high latitudes spend the winter in a state of overwintering diapause, which is characterized by arrested reproduction, reduced food intake and metabolism, increased life span. The main trigger to enter diapause decreasing day length summer-autumn. It thus assumed that circadian clock acts as an internal sensor for measuring photoperiod orchestrates appropriate seasonal changes physiology metabolism through various neurohormones. However, little known about neuronal organization network neurosecretory system controls high-latitude insects. We addressed this here mapping expression proteins neuropeptides/neurohormones fly Drosophila littoralis. found principal both systems similar melanogaster, but with some striking differences neuropeptide levels patterns. small ventrolateral neurons express pigment-dispersing factor (PDF) short F (sNPF) are most important robust rhythmicity D. melanogaster virtually lack PDF sNPF In contrast, dorsolateral ion transport peptide additionally allatostatin-C appear suited transfer day-length information lateral cells littoralis contain more neuropeptides than melanogaster. Among them, coexpress corazonin, PDF, diuretic hormone 44 control diapause. Our work sets stage investigate roles these diverse regulating insect

Язык: Английский

Процитировано

6
Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations DOI Creative Commons
Sergio Hidalgo, Joanna C. Chiu

Journal of Comparative Physiology A, Год журнала: 2023, Номер 210(4), С. 585 - 599

Опубликована: Авг. 16, 2023

Abstract Organisms adapt to unfavorable seasonal conditions survive. These adaptations rely on the correct interpretation of environmental cues such as photoperiod, and temperature. Genetic studies in several organisms, including genetic powerhouse Drosophila melanogaster , indicate that circadian clock components, period timeless are involved photoperiodic-dependent adaptations, but our understanding this process is far from complete. In particular, role temperature a key factor complement photoperiodic response not well understood. The development new sequencing technologies has proven extremely useful plastic changes other cellular components undergo different conditions, gene expression alternative splicing. This article discusses integration photoperiod for biology downstream molecular pathways regulation physiological occur with changing seasons. We focus discussion current involvement neuronal circuits these D. .

Язык: Английский

Процитировано

5
Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster DOI Creative Commons
Qiangqiang Jia, Liu Yang,

Jiamin Wen

и другие.

BMC Biology, Год журнала: 2024, Номер 22(1)

Опубликована: Май 13, 2024

Abstract Background Juvenile hormones (JH) play crucial role in regulating development and reproduction insects. The most common form of JH is III, derived from MF through epoxidation by CYP15 enzymes. However, the higher dipterans, such as fruitfly, Drosophila melanogaster , a bis-epoxide JHB3, accounted detected. Moreover, these dipterans have lost gene their genomes. As result, identity P450 epoxidase biosynthesis pathway remains unknown. Results In this study, we show that Cyp6g2 serves major responsible for JHB3 III D. . predominantly expressed corpus allatum (CA), concurring with expression pattern jhamt another well-studied last steps biosynthesis. Mutation leads to severe disruptions larval-pupal metamorphosis exhibits reproductive deficiencies, exceeding those seen mutants. Notably, −/− ::jhamt 2 double mutants all died at pupal stage but could be rescued topical application analogs. titer analyses revealed both mutant lacking while overexpression or caused significant increase titer. Conclusions These findings collectively established laid groundwork further understanding pave way developing specific inhibitors insect growth regulators insecticides.

Язык: Английский

Процитировано

1
Gut-to-brain regulation ofDrosophilaaging through neuropeptide F, insulin and juvenile hormone DOI
Jiangtian Chen, Marcela Nouzová, Fernando G. Noriega

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 30, 2024

Dietary restriction slows aging in many animals, while some cases the sensory signals from diet alone are sufficient to retard or accelerate lifespan. The digestive tract is a candidate location sense nutrients, where neuropeptides secreted by enteroendocrine cells (EEC) produce systemic response food. Here we measure how

Язык: Английский

Процитировано

1