PLoS ONE,
Год журнала:
2024,
Номер
19(12), С. e0311884 - e0311884
Опубликована: Дек. 4, 2024
Thallium
(Tl)
is
a
heavy
metal
with
toxicity
comparative
to
other
metals
such
as
As,
Cd,
and
Hg.
Nevertheless,
fewer
studies
have
been
reported
concerning
the
molecular
mechanism
of
Tl
compared
metals.
To
obtain
insight
into
in
kidney,
rats
were
intraperitoneally
administered
2
SO
4
(30
mg/kg),
kidneys
removed
or
5
days
later
examine
effects
Tl.
Transcriptome
analysis
using
DNA
microarray
rat
kidney
after
administration
showed
that
cytoplasmic
ribosomal
proteins
are
most
upregulated
category;
many
genes
involved
ribosome
biosynthesis
by
administration.
This
upregulation
was
associated
activation
eukaryotic
transcription
initiation
factor
2α
(eIF2α),
implying
increased
biogenesis
linked
subsequent
protein
translation.
In
contrast,
decreased
mitochondrial
revealed
via
proteomic
analysis.
Although
we
found
an
increase
Myc,
positive
regulator
both
biogenesis,
levels
NRF1
TFAM,
regulators
whose
gene
expression
directory
activated
paradoxically
observed.
Taken
together,
differing
responses
ribosomes
mitochondria
Failure
transmission
Myc
signal
NRF1/TFAM
might
be
observed
disruption
coordinated
during
kidney.
Philosophical Transactions of the Royal Society B Biological Sciences,
Год журнала:
2025,
Номер
380(1921)
Опубликована: Март 6, 2025
Since
the
framing
of
Central
Dogma,
it
has
been
speculated
that
physically
distinct
ribosomes
within
cells
may
influence
gene
expression
and
cellular
physiology.
While
heterogeneity
in
ribosome
composition
reported
bacteria,
protozoans,
fungi,
zebrafish,
mice
humans,
its
functional
implications
remain
actively
debated.
Here,
we
review
recent
evidence
demonstrating
conserved
variant
ribosomal
DNA
(rDNA)
alleles
humans
renders
their
translating
pool
intrinsically
heterogeneous
at
level
RNA
(rRNA).
In
this
context,
discuss
reports
nutrient
limitation-induced
stress
Escherichia
coli
leads
to
changes
rRNA
allele
expression,
programmatically
altering
transcription
phenotype.
We
highlight
expressing
from
operons
exhibit
drug
sensitivities,
which
can
be
recapitulated
vitro
potentially
rationalized
by
subtle
perturbations
structure
or
dynamic
properties.
Finally,
differential
rDNA
results
different
populations
subtypes
mammalian
tissues.
These
findings
motivate
further
research
into
impacts
heterogeneities
on
function
predict
strategies
targeting
hold
therapeutic
potential.This
article
is
part
discussion
meeting
issue
'Ribosome
diversity
impact
protein
synthesis,
development
disease'.
Clinical Epigenetics,
Год журнала:
2024,
Номер
16(1)
Опубликована: Янв. 24, 2024
Abstract
Background
Periodontitis
is
a
highly
prevalent
oral
disease
characterized
by
bacterium-induced
periodontal
inflammation
and
alveolar
bone
destruction.
Osteoblast
function
impaired
in
periodontitis
with
global
proteome
change.
METTL3
the
pivotal
methyltransferase
of
N
6
-methyladenosine
(m
A)
that
recently
proved
to
exert
crucial
role
osteoblast
differentiation.
This
study
aims
investigate
ribosome
biogenesis
progression.
Results
was
knocked
down
osteoblasts,
downregulated
genes
were
enriched
translation.
knockdown
inhibited
oxidative
phosphorylation
LPS-stimulated
whereas
overexpression
facilitated
ribosomal
mitochondrial
function.
Mechanistically,
mediated
biological
behaviors
activating
Wnt/β-catenin/c-Myc
signaling.
depletion
enhanced
mRNA
expression
stability
Dkk3
Sostdc1
via
YTHDF2.
In
mice,
inhibitor
SAH
promoted
loss
local
inflammatory
status,
which
partially
rescued
Wnt/β-catenin
pathway
activator
CHIR-99021
HCl.
Conclusions
signaling
LPS-treated
osteoblasts
alleviated
destruction
mice.
ACS Sensors,
Год журнала:
2024,
Номер
9(3), С. 1545 - 1554
Опубликована: Март 7, 2024
rRNAs
are
prevalent
in
living
organisms.
They
produced
nucleolus
and
mitochondria
play
essential
cellular
functions.
In
addition
to
the
primary
biofunction
protein
synthesis,
have
been
recognized
as
emerging
signaling
molecule
drug
target
for
studies
on
morphology,
mitochondrial
autophagy,
tumor
cell
malignancy.
Currently,
only
a
few
rRNA-selective
probes
developed,
most
of
them
encounter
drawbacks
low
water
solubility,
poor
nuclear
membrane
permeability,
short
emission
wavelength,
stability
against
photobleaching,
high
cytotoxicity.
These
unfavorable
properties
rRNA
limit
their
potential
applications.
present
study,
we
reported
new
near-infrared
fluorescent
turn-on
probe,
4MPS-TO,
capable
tracking
live
human
cancer
cells.
The
real-time
monitoring
performance
morphology
autophagy
is
demonstrated
HeLa
probe
shows
great
application
being
used
rRNA-selective,
sensitive,
photostable
imaging
tool
chemical
biology
study
screening.
Journal of Cancer,
Год журнала:
2025,
Номер
16(4), С. 1149 - 1166
Опубликована: Янв. 6, 2025
KANK2,
a
gene
crucial
for
cell
migration
and
movement,
is
implicated
in
neoplastic
non-neoplastic
diseases.
This
study
aimed
to
analyze
KANK2's
expression
its
diagnostic
prognostic
significance
across
33
cancers
using
multiple
online
databases.
comprehensively
KANK2
The
Cancer
Genome
Atlas
(TCGA)
Genotype-Tissue
Expression
(GTEx)
database.
Multiple
web
platforms
software
were
used
data
analysis,
including
R,
Cytoscape,
HPA,
TISIDB,
UALCAN,
GEO,
cBioPortal,
STRING,
GSCALite,
CancerSEA.
WB
qPCR
experiments
verify
the
results.
widely
expressed
various
tissues
has
significant
value
cancers,
with
AUC
values
exceeding
0.75
13
cancer
types.
Survival
analysis
indicated
that
significantly
associated
overall
survival
(OS)
disease-specific
(DSS)
several
cancers.
varied
different
molecular
immune
subtypes
was
specific
genetic
mutations
DNA
methylation
patterns.
Functional
state
highlighted
correlations
processes
such
as
EMT,
angiogenesis,
apoptosis.
GSEA
identified
pathways
related
proliferation,
migration,
extracellular
matrix
remodeling.
key
interacting
proteins
by
PPI
network
sensitive
drug
molecules
found
GSCA
results
also
confirmed
two
GEO
datasets
serves
valuable
biomarker
diagnosis
prognosis
intricately
linked
cellular
processes,
offering
potential
therapeutic
targets
future
research.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 14, 2025
Low
fertility
in
cows
leads
to
early
removal
from
herds.
Since
reproductive
traits
are
complex
and
have
low
heritability,
genetic
analysis
can
aid
improving
reproduction.
This
study
identified
key
genes
linked
by
conducting
genome-
transcriptome-wide
association
studies,
RNA-seq
analysis,
meta-analysis,
weighted
gene
co-expression
network
functional
enrichment
analysis.
Through
these
methods,
we
candidate
related
Cow
conception
rate
(CCR),
Daughter
pregnancy
(DPR),
Heifer
(HCR),
overall
traits,
helping
improve
selection
for
success
cows.
The
include
RPL12,
FKBP1B,
FZD10,
COX10,
COX7A2,
GAA,
ETFBKMT,
ACSM5,
NUDT9,
TIGAR,
PAIP2,
PSMB5.
Notably,
COX7A2
involved
the
"generation
of
precursor
metabolites
energy"
process.
TIGAR
exhibit
significant
impacts
on
CCR,
DPR,
HCR.
PSMB5
been
as
hub
traits.
RPL12
plays
a
role
protein
synthesis,
essential
gametogenesis
embryo
development,
while
FKBP1B
regulates
calcium
signaling,
particularly
oocyte
aging
decline,
FZD10
is
crucial
Wnt
signaling.
serve
markers
genomic
aimed
at
enhancing
cow.
Ribosome
biogenesis
is
vital
for
sustaining
stem
cell
properties,
yet
its
regulatory
mechanisms
are
obscure.
Herein,
we
show
unique
properties
of
zebrafish
meioc
mutants
in
which
spermatogonial
cells
(SSCs)
do
not
differentiate
or
upregulate
rRNAs.
Meioc
colocalized
with
Piwil1
perinuclear
germ
granules,
but
depletion
resulted
accumulation
nucleoli.
Nucleolar
interacted
45S
pre-rRNA.
piwil1
+/−
spermatogonia
reduced
upregulated
rRNAs,
and
;meioc
−/−
recovered
differentiation
later
than
those
.
Further,
Setdb1
HP1α,
exhibited
high
levels
H3K9me3
methylated
CpG
the
45S-rDNA
region.
These
results
indicate
that
SSCs
silence
rRNA
transcription
repressive
marks
similar
to
Drosophila
piRNA
targets
RNA
polymerase
II,
has
a
function
on
preventing
localization
nucleoli
transcripts
promote
SSC
differentiation.
Ribosome
biogenesis
is
vital
for
sustaining
stem
cell
properties,
yet
its
regulatory
mechanisms
are
obscure.
Herein,
we
show
unique
properties
of
zebrafish
meioc
mutants
in
which
spermatogonial
cells
(SSCs)
do
not
differentiate
or
upregulate
rRNAs.
Meioc
colocalized
with
Piwil1
perinuclear
germ
granules,
but
depletion
resulted
accumulation
nucleoli.
Nucleolar
interacted
45S
pre-rRNA.
piwil1
+/−
spermatogonia
reduced
upregulated
rRNAs,
and
;meioc
−/−
recovered
differentiation
later
than
those
.
Further,
Setdb1
HP1α,
exhibited
high
levels
H3K9me3
methylated
CpG
the
45S-rDNA
region.
These
results
indicate
that
SSCs
silence
rRNA
transcription
repressive
marks
similar
to
Drosophila
piRNA
targets
RNA
polymerase
II,
has
a
function
on
preventing
localization
nucleoli
transcripts
promote
SSC
differentiation.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 20, 2025
Background
Methicillin-resistant
Staphylococcus
aureus
(MRSA)
poses
a
significant
clinical
challenge
due
to
its
multidrug
resistance.
Diacerein
(DIA),
primarily
used
treat
degenerative
joint
diseases,
has
recently
been
found
exhibit
antibacterial
activity,
though
specific
mechanisms
remain
unclear.
Methods
The
minimum
inhibitory
concentration
(MIC)
and
bactericidal
(MBC)
of
DIA,
as
well
in
-
vitro
combination
susceptibility
testing,
were
determined
using
the
broth
microdilution
method.
Additionally,
resistance
induction
assays,
time-growth
curve
measurements,
membrane
fluidity,
intracellular
protein
levels,
reactive
oxygen
species
(ROS)
assessed.
inhibition
clearance
MRSA
biofilms
by
DIA
evaluated
crystal
violet
staining
method,
with
bacterial
morphology
observed
via
scanning
electron
microscopy
confocal
laser
microscopy.
Finally,
transcriptome
analysis
was
conducted
identify
gene
expression
changes
treated
RT-qPCR
verification
performed.
Results
MIC
MBC
against
32
μg/mL
128
μg/mL,
respectively,
synergistic
effects
when
combined
ampicillin.
increased
ROS
levels
fluidity
MRSA,
decreased
soluble
synthesis,
altered
morphology.
significantly
inhibited
biofilm
formation
disrupted
pre
existing
biofilms.
Transcriptome
revealed
1,045
differentially
expressed
genes
between
DIA-treated
group
control
group,
involving
pathways
such
tricarboxylic
acid
cycle,
phosphorylation,
ribosome
metabolism,
nucleotide
metabolism.
Conclusion
In
summary,
anti-biofilm
activities
does
not
easily
induce
Its
may
involve
multiple
aspects,
including
energy
