Chloride
is
the
most
abundant
anion
in
cell
physiology
and
plays
many
critical
roles
maintaining
cellular
homeostasis.
However,
current
chloride
sensors
are
rare,
with
inherent
sensitivity
their
emission
properties,
such
as
vulnerability
to
pH
changes
or
short
lifetimes.
These
limitations
restrict
application
aqueous
media
imaging.
In
this
work,
we
employed
a
transition
metal
complex
bearing
pyridinium
recognition
unit
for
studied
phosphorescence
properties.
Iridium(III)
1
was
synthesized
an
alternative
chloride-sensitive
luminophore.
The
conjugable
design
also
allows
customization
desired
applications.
Complex
exhibited
high
selectivity
sensing
across
different
physiological
environments,
regardless
of
fluctuation
ionic
strength.
Additionally,
featured
long
microsecond
lifetime.
ability
can
be
measured
through
both
luminescence
intensity
long-lived
phosphorescent
lifetime
simultaneously,
providing
potential
route
ACS Nano,
Год журнала:
2024,
Номер
18(19), С. 12168 - 12186
Опубликована: Апрель 30, 2024
Assessment
of
hypoxia,
nutrients,
metabolite
gradients,
and
other
hallmarks
the
tumor
microenvironment
within
3D
multicellular
spheroid
organoid
models
represents
a
challenging
analytical
task.
Here,
we
report
red/near-infrared
(NIR)
emitting
cell
staining
with
O2-sensitive
nanoparticles,
which
enable
measurements
oxygenation
on
conventional
fluorescence
microscope.
Nanosensor
probes,
termed
"MMIR"
(multimodal
infrared),
incorporate
an
NIR
metalloporphyrin
(PtTPTBPF)
deep
red
aza-BODIPY
reference
dyes
biocompatible
polymer
shell,
allowing
for
oxygen
gradient
quantification
via
ratio
phosphorescence
lifetime
readouts.
We
optimized
techniques
evaluated
nanosensor
probe
characteristics
cytotoxicity.
Subsequently,
applied
nanosensors
to
live
based
HCT116,
DPSCs,
SKOV3
cells,
at
rest,
treated
drugs
affecting
respiration.
found
that
growth
medium
viscosity,
size,
formation
method
influenced
oxygenation.
Some
spheroids
produced
from
HCT116
dental
pulp
stem
cells
exhibited
"inverted"
higher
core
levels
than
periphery.
This
contrasted
frequently
encountered
"normal"
hypoxia
toward
caused
by
diffusion.
Further
microscopy
analysis
demonstrated
metabolic
stratification
spheroids:
thus,
autofluorescence
FLIM
NAD(P)H
indicated
glycolytic
localization
OxPhos-active
Collectively,
demonstrate
strong
potential
NIR-emitting
ratiometric
advanced
studies
targeting
quantitative
real-time
monitoring
metabolism
in
complex
tissue
models.
Current Opinion in Solid State and Materials Science,
Год журнала:
2024,
Номер
30, С. 101147 - 101147
Опубликована: Март 18, 2024
Fluorescence
lifetime
imaging
microscopy
(FLIM)
is
a
powerful
tool
offering
molecular
specific
insights
into
samples
through
the
measurement
of
fluorescence
decay
time,
with
promising
applications
in
diverse
research
fields.
However,
to
acquire
two-dimensional
images,
conventional
FLIM
relies
on
extensive
scanning
both
spatial
and
temporal
domain,
resulting
much
slower
acquisition
rates
compared
intensity-based
approaches.
This
problem
further
magnified
three-dimensional
imaging,
as
it
necessitates
additional
along
depth
axis.
Recent
advancements
have
aimed
enhance
speed
capabilities
FLIM.
review
explores
progress
made
addressing
these
challenges
discusses
potential
directions
for
future
developments
instrumentation.
Chloride
is
the
most
abundant
anion
in
cells
and
plays
many
critical
roles
maintaining
cellular
homeostasis.
However,
current
chloride
indicators
are
rare
with
inherent
sensitivity
their
emission
properties,
such
as
vulnerability
to
pH
changes
or
short
lifetimes.
These
limitations
restrict
application
aqueous
media
imaging.
In
this
work,
we
employed
a
transition-metal
complex
bearing
pyridinium
recognition
unit
for
studied
phosphorescence
properties.
Iridium(III)
1
was
synthesized
an
alternative
chloride-sensitive
luminophore.
The
conjugable
design
also
allows
customization
desired
applications.
Complex
exhibited
high
selectivity
sensing
across
different
physiological
environments,
regardless
of
fluctuation
ionic
strength.
Additionally,
featured
microsecond
lifetime.
ability
can
be
measured
through
both
luminescence
intensity
long-lived
phosphorescent
lifetime,
providing
potential
route
analogue
1b
successfully
applied
imaging
Cl-
environments
showed
dose-dependent
responses
live
fixed
cells.
Journal of Biomedical Optics,
Год журнала:
2022,
Номер
27(02)
Опубликована: Фев. 25, 2022
Biomedical
optics
system
design,
image
formation,
and
analysis
have
primarily
been
guided
by
classical
physical
modeling
signal
processing
methodologies.
Recently,
however,
deep
learning
(DL)
has
become
a
major
paradigm
in
computational
demonstrated
utility
numerous
scientific
domains
various
forms
of
data
analysis.
Journal of Biomedical Optics,
Год журнала:
2024,
Номер
29(02)
Опубликована: Фев. 29, 2024
SignificanceFluorescence
lifetime
imaging
(FLI)
plays
a
pivotal
role
in
enhancing
our
understanding
of
biological
systems,
providing
valuable
tool
for
non-invasive
exploration
biomolecular
and
cellular
dynamics,
both
vitro
vivo.
Its
ability
to
selectively
target
multiplex
various
entities,
alongside
heightened
sensitivity
specificity,
offers
rapid
cost-effective
insights.AimOur
aim
is
investigate
the
multiplexing
capabilities
near-infrared
(NIR)
FLI
within
scattering
medium
that
mimics
tissues.
We
strive
develop
comprehensive
FLI's
potential
diverse
targets
complex,
tissue-like
environment.ApproachWe
introduce
an
innovative
Monte
Carlo
(MC)
simulation
approach
accurately
describes
behavior
fluorescent
photons
turbid
media.
Applying
phasor
analyses,
we
enable
distinct
single
image.
Leveraging
state-of-the-art
single-photon
avalanche
diode
(SPAD)
time-gated
camera,
SPAD512S,
conduct
experimental
wide-field
NIR
regime.ResultsOur
study
demonstrates
successful
dual
image,
reaching
depth
1
cm
phantoms.
Through
novel
MC
showcase
effectiveness
methodology
overcoming
challenges
posed
by
media.ConclusionsThis
research
underscores
applications
complex
environments.
By
combining
advanced
techniques
with
cutting-edge
tools,
significant
results
advance
field
research.
Biomedical Optics Express,
Год журнала:
2025,
Номер
16(3), С. 1006 - 1006
Опубликована: Фев. 5, 2025
We
developed
a
novel
methodology
for
manufacturing
multimodal,
tissue-mimicking
phantoms
that
exhibit
both
molecular
and
biomechanical
contrast.
This
leverages
the
immiscibility
of
silicone
hydrogels
to
create
solid
mesoscale
with
localized
regions
precisely
controlled
fluorescence,
including
fluorescence
lifetime
properties,
adjustable
stiffness,
without
requiring
physical
barriers.
Mechanical,
fluorescent,
optical
characterization
confirmed
tunability
across
range
values
relevant
biomedical
applications.
A
macroscale
3D
phantom
was
fabricated,
its
properties
were
validated
through
imaging
(FLI)
coherence
elastography
(OCE).
Validation
demonstrated
successful
tuning
mechanical
contrasts
within
structure,
highlighting
feasibility
multimodal
FLI-OCE.
new
process
is
expected
support
development
validation
approaches
study
tumor
microenvironment
(TME),
as
well
their
impact
on
therapeutic
efficacy,
enhance
targeted
therapies.
