With or without you: gut microbiota does not predict aggregation behavior in European earwig females

Behavioral Ecology, Год журнала: 2024, Номер 35(3)

Опубликована: Март 28, 2024

Abstract The reasons why some individuals are solitary, and others gregarious the subject of ongoing debate as we seek to understand emergence sociality. Recent studies suggest that expression aggregation behaviors may be linked gut microbiota host. Here, tested this hypothesis in females European earwig. This insect is ideal for addressing question, adults both naturally vary degree which they live groups show interindividual variation their microbial communities. We video-tracked 320 field-sampled quantify natural then whether most least had different microbiota. also compared general activity, boldness, body size, condition these examined association between each traits Contrary our predictions, found no difference females. There was two types Independent aggregation, overall associated with female condition, but not any other measurements. Overall, results demonstrate a host’s necessarily major driver or consequence behavior species inter-individual group living call future investigate determinants role earwigs.

Язык: Английский

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From worms to humans: Understanding intestinal lipid metabolism via model organisms

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Год журнала: 2023, Номер 1868(4), С. 159290 - 159290

Опубликована: Фев. 3, 2023

Язык: Английский

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Wolbachia impacts microbiome diversity and fitness‐associated traits for Drosophila melanogaster in a seasonally fluctuating environment

Ecology and Evolution, Год журнала: 2024, Номер 14(7)

Опубликована: Июль 1, 2024

Abstract The microbiome contributes to many different host traits, but its role in adaptation remains enigmatic. fitness benefits of the often depend on ecological conditions, theory suggests that fluctuations both and environment modulate these benefits. Moreover, vertically transmitted bacteria might constrain ability respond changing environments. Drosophila melanogaster provides an excellent system investigate impacts interactions between environment. To address this question, we created field mesocosms D. undergoing seasonal environmental change with without bacteria, Wolbachia pipientis . Sampling temporal patterns revealed constrained microbial diversity. Furthermore, a dominant member microbiome, Commensalibacter , were associated differences two higher‐order starvation resistance lifespan. Our work here interplay abiotic context microbe–microbe may shape key phenotypes underlie We conclude by exploring consequences complex for our understanding eco‐evolutionary processes host‐microbiome interactions.

Язык: Английский

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The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis

Metabolites, Год журнала: 2021, Номер 11(5), С. 298 - 298

Опубликована: Май 6, 2021

The host microbiome plays an important role in regulating physiology through microbiota-derived metabolites during host-microbiome interactions. However, molecular mechanism underly interactions remains to be explored. In this study, we used Drosophila as the model investigate influence of and metabolite sodium butyrate on transcriptome metabolome. We established both a sterile conventional demonstrate butyrate. Using multi-omics analysis, found that could impact gene expression patterns model. analysis gut microbial using 16S rRNA sequencing showed treatment also influenced bacterial structures. addition, identified by ultra-high performance liquid chromatography-MS/MS were shown affected with lipids dominant changed components. Our integrative transcriptome, microbiome, metabolome data candidate transcripts are coregulated Taken together, our results reveal metabolome, work provides better understanding at level data.

Язык: Английский

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Microbial Community Dynamics in Natural Drosophila melanogaster Populations Across Seasons

Environmental Microbiology, Год журнала: 2025, Номер 27(6)

Опубликована: Июнь 1, 2025

ABSTRACT Many insects benefit from gut microbes that contribute to digestion, detoxification, nutrient supplementation or defence. Although abiotic and biotic factors are known shape insect‐associated microbial communities, the seasonal dynamics their potential impact on host fitness remain poorly studied. Here we investigated temporal changes in bacterial fungal communities associated with model organism Drosophila melanogaster over 5 months. Our results reveal high inter‐individual variation, but also consistent of three wild D. populations early spring late summer. These were driven by specific indicator species, particularly Acetobacteraceae bacteria ( Gluconobacter Komagataeibacter ) Saccharomycetales yeasts Pichia , Starmerella Kregervanrija Hanseniaspora Saccharomycopsis Priceomyces Dipodascopsis ). The not accompanied differences total abundance, alpha‐diversity only changed across sampling months for communities. While ‐associated likely exposure seasonally changing environments diets, they may have important impacts fitness. Elucidating adaptive value will enhance our understanding how symbiotic ecological niche shifts geographic range expansions insects.

Язык: Английский

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Predicted Metabolic Function of the Gut Microbiota of Drosophila melanogaster

mSystems, Год журнала: 2021, Номер 6(3)

Опубликована: Май 10, 2021

Drosophila is an important model for microbiome research partly because of the low complexity its mostly culturable gut microbiota. Our current understanding how interacts with microbes and these interactions influence host traits derives almost entirely from empirical studies that focus on individual microbial taxa or classes metabolites.

Язык: Английский

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Bugs on Drugs: A Drosophila melanogaster Gut Model to Study In Vivo Antibiotic Tolerance of E. coli

Microorganisms, Год журнала: 2022, Номер 10(1), С. 119 - 119

Опубликована: Янв. 7, 2022

With an antibiotic crisis upon us, we need to boost development and improve antibiotics' efficacy. Crucial is knowing how efficiently kill bacteria, especially in more complex vivo conditions. Indeed, many bacteria harbor antibiotic-tolerant persisters, variants that survive exposure our most potent antibiotics catalyze resistance development. However, persistence often only studied vitro as lack flexible models. Here, I explored the potential of using Drosophila melanogaster a model for antimicrobial research, combining methods with microbiology techniques: assessing fly feeding, generating germ-free or bacteria-associated situ microscopy. Adult flies tolerate at high doses, although larvae show impaired Orally presented E. coli associates mostly resides crop. shows overall tolerance potentially resulting from heterogeneity growth rates. The hipA7 high-persistence mutant displays increased survival while expected low ΔrelAΔspoT ΔrpoS mutants cannot be confirmed vivo. In conclusion, research offers system test findings assays broader, condition.

Язык: Английский

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Microbiota-mediated competition between Drosophila species

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Сен. 7, 2023

The influence of microbiota in ecological interactions, and particular competition, is poorly known. We studied competition between two insect species, the invasive pest Drosophila suzukii model melanogaster, whose larval niches overlap ripe, but not rotten, fruit.We discovered D. females prevent costly interspecific by avoiding oviposition on substrates previously visited melanogaster. More precisely, melanogaster association with gut bacteria genus Lactobacillus triggered avoidance. However, avoidance behavior condition-dependent, that themselves carry stop sites adaptive significance cues from competitor's was revealed experimentally reproducing in-fruit competition: reduced survival larvae only occurred if competitor had its normal microbiota.This study establishes microbiotas as potent mediators reveals a central role for context-dependent behaviors under bacterial influence. Video Abstract.

Язык: Английский

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Genome-Inferred Correspondence between Phylogeny and Metabolic Traits in the Wild Drosophila Gut Microbiome

Genome Biology and Evolution, Год журнала: 2021, Номер 13(8)

Опубликована: Июнь 2, 2021

Annotated genome sequences provide valuable insight into the functional capabilities of members microbial communities. Nevertheless, most studies on microbiome in animal guts use metagenomic data, hampering assignment genes to specific taxa. Here, we make readily culturable bacterial communities gut fruit fly Drosophila melanogaster obtain draft for 96 isolates from wild flies. These include 81 new de novo assembled genomes, assigned three orders (Enterobacterales, Lactobacillales, and Rhodospirillales) with 80% strains identified species level using average nucleotide identity phylogenomic reconstruction. Based annotations by RAST pipeline, among-isolate variation metabolic function partitioned strongly order, particularly amino acid metabolism (Rhodospirillales), fermentation, (Lactobacillales) arginine, urea, polyamine (Enterobacterales). Seven species, comprising 2-3 each were well-represented among included ≥5 strains, permitting analysis functions accessory (i.e., not present every strain). Overall, order. Two Gluconobacter cerinus (Rhodospirillales) Lactiplantibacillus plantarum had large dominated (G. cerinus) carbohydrate (La. plantarum). The patterns at multiple phylogenetic scales basis future ecological evolutionary processes shaping diversity microorganisms associated natural populations Drosophila.

Язык: Английский

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Insect Lipid Metabolism in the Presence of Symbiotic and Pathogenic Viruses and Bacteria

Advances in experimental medicine and biology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

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