The Predicted Metabolic Function of the Gut Microbiota ofDrosophila melanogaster DOI Open Access
Nana Y. D. Ankrah, Brandon Barker, Joan Song

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Jan. 20, 2021

ABSTRACT An important goal for many nutrition-based microbiome studies is to identify the metabolic function of microbes in complex microbial communities and its impact on host physiology. This research can be confounded by poorly-understood effects community composition diet traits individual taxa. Here, we investigated these multi-way interactions constructing analyzing models comprising every combination five bacterial members Drosophila gut (from single taxa five-member Acetobacter Lactobacillus species) under three nutrient regimes. We show that bacteria dynamic, influenced responsive dietary modulation. Furthermore, ecological such as competition mutualism identified from growth patterns are underlain a diversity interactions, tend compete amino acids B vitamins more frequently than carbon sources. Our results reveal addition fermentation products acetate, intermediates tricarboxylic acid (TCA) cycle including 2-oxoglutarate succinate produced at high flux cross-fed between suggesting roles TCA modulating microbe potential influence traits. These provide specific predictions among different regimes, with potentially consequences overall nutritional host. IMPORTANCE an model partly because low complexity mostly culturable microbiota. current understanding how interacts derives almost entirely empirical focus or classes metabolites. have failed capture fully occur microbe. To overcome this limitation, reconstructed analyzed 31 principal . revealed highly dynamic co-occurring availability. generate testable hypothesis about among-microbe metabolites available

Language: Английский

With or without you: gut microbiota does not predict aggregation behavior in European earwig females DOI
Marie‐Charlotte Cheutin,

Benjamin LECLERC,

J. Meunier

et al.

Behavioral Ecology, Journal Year: 2024, Volume and Issue: 35(3)

Published: March 28, 2024

Abstract The reasons why some individuals are solitary, and others gregarious the subject of ongoing debate as we seek to understand emergence sociality. Recent studies suggest that expression aggregation behaviors may be linked gut microbiota host. Here, tested this hypothesis in females European earwig. This insect is ideal for addressing question, adults both naturally vary degree which they live groups show interindividual variation their microbial communities. We video-tracked 320 field-sampled quantify natural then whether most least had different microbiota. also compared general activity, boldness, body size, condition these examined association between each traits Contrary our predictions, found no difference females. There was two types Independent aggregation, overall associated with female condition, but not any other measurements. Overall, results demonstrate a host’s necessarily major driver or consequence behavior species inter-individual group living call future investigate determinants role earwigs.

Language: Английский

Citations

7
From worms to humans: Understanding intestinal lipid metabolism via model organisms DOI Creative Commons
Darby W. Kozan, Joshua T. Derrick, William B. Ludington

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2023, Volume and Issue: 1868(4), P. 159290 - 159290

Published: Feb. 3, 2023

Language: Английский

Citations

12
Wolbachia impacts microbiome diversity and fitness‐associated traits for Drosophila melanogaster in a seasonally fluctuating environment DOI Creative Commons
Lucas P. Henry, Michael Fernández, Scott Wolf

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract The microbiome contributes to many different host traits, but its role in adaptation remains enigmatic. fitness benefits of the often depend on ecological conditions, theory suggests that fluctuations both and environment modulate these benefits. Moreover, vertically transmitted bacteria might constrain ability respond changing environments. Drosophila melanogaster provides an excellent system investigate impacts interactions between environment. To address this question, we created field mesocosms D. undergoing seasonal environmental change with without bacteria, Wolbachia pipientis . Sampling temporal patterns revealed constrained microbial diversity. Furthermore, a dominant member microbiome, Commensalibacter , were associated differences two higher‐order starvation resistance lifespan. Our work here interplay abiotic context microbe–microbe may shape key phenotypes underlie We conclude by exploring consequences complex for our understanding eco‐evolutionary processes host‐microbiome interactions.

Language: Английский

Citations

4
The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis DOI Creative Commons
Fan Zhou, Biaodi Liu, Xin Liu

et al.

Metabolites, Journal Year: 2021, Volume and Issue: 11(5), P. 298 - 298

Published: May 6, 2021

The host microbiome plays an important role in regulating physiology through microbiota-derived metabolites during host-microbiome interactions. However, molecular mechanism underly interactions remains to be explored. In this study, we used Drosophila as the model investigate influence of and metabolite sodium butyrate on transcriptome metabolome. We established both a sterile conventional demonstrate butyrate. Using multi-omics analysis, found that could impact gene expression patterns model. analysis gut microbial using 16S rRNA sequencing showed treatment also influenced bacterial structures. addition, identified by ultra-high performance liquid chromatography-MS/MS were shown affected with lipids dominant changed components. Our integrative transcriptome, microbiome, metabolome data candidate transcripts are coregulated Taken together, our results reveal metabolome, work provides better understanding at level data.

Language: Английский

Citations

24
Predicted Metabolic Function of the Gut Microbiota of Drosophila melanogaster DOI Creative Commons
Nana Y. D. Ankrah, Brandon Barker, Joan Song

et al.

mSystems, Journal Year: 2021, Volume and Issue: 6(3)

Published: May 10, 2021

Drosophila is an important model for microbiome research partly because of the low complexity its mostly culturable gut microbiota. Our current understanding how interacts with microbes and these interactions influence host traits derives almost entirely from empirical studies that focus on individual microbial taxa or classes metabolites.

Language: Английский

Citations

17
Bugs on Drugs: A Drosophila melanogaster Gut Model to Study In Vivo Antibiotic Tolerance of E. coli DOI Creative Commons
Bram Van den Bergh

Microorganisms, Journal Year: 2022, Volume and Issue: 10(1), P. 119 - 119

Published: Jan. 7, 2022

With an antibiotic crisis upon us, we need to boost development and improve antibiotics' efficacy. Crucial is knowing how efficiently kill bacteria, especially in more complex vivo conditions. Indeed, many bacteria harbor antibiotic-tolerant persisters, variants that survive exposure our most potent antibiotics catalyze resistance development. However, persistence often only studied vitro as lack flexible models. Here, I explored the potential of using Drosophila melanogaster a model for antimicrobial research, combining methods with microbiology techniques: assessing fly feeding, generating germ-free or bacteria-associated situ microscopy. Adult flies tolerate at high doses, although larvae show impaired Orally presented E. coli associates mostly resides crop. shows overall tolerance potentially resulting from heterogeneity growth rates. The hipA7 high-persistence mutant displays increased survival while expected low ΔrelAΔspoT ΔrpoS mutants cannot be confirmed vivo. In conclusion, research offers system test findings assays broader, condition.

Language: Английский

Citations

9
Genome-Inferred Correspondence between Phylogeny and Metabolic Traits in the Wild Drosophila Gut Microbiome DOI Creative Commons
John G. McMullen,

Eduardo Bueno,

Frances Blow

et al.

Genome Biology and Evolution, Journal Year: 2021, Volume and Issue: 13(8)

Published: June 2, 2021

Annotated genome sequences provide valuable insight into the functional capabilities of members microbial communities. Nevertheless, most studies on microbiome in animal guts use metagenomic data, hampering assignment genes to specific taxa. Here, we make readily culturable bacterial communities gut fruit fly Drosophila melanogaster obtain draft for 96 isolates from wild flies. These include 81 new de novo assembled genomes, assigned three orders (Enterobacterales, Lactobacillales, and Rhodospirillales) with 80% strains identified species level using average nucleotide identity phylogenomic reconstruction. Based annotations by RAST pipeline, among-isolate variation metabolic function partitioned strongly order, particularly amino acid metabolism (Rhodospirillales), fermentation, (Lactobacillales) arginine, urea, polyamine (Enterobacterales). Seven species, comprising 2-3 each were well-represented among included ≥5 strains, permitting analysis functions accessory (i.e., not present every strain). Overall, order. Two Gluconobacter cerinus (Rhodospirillales) Lactiplantibacillus plantarum had large dominated (G. cerinus) carbohydrate (La. plantarum). The patterns at multiple phylogenetic scales basis future ecological evolutionary processes shaping diversity microorganisms associated natural populations Drosophila.

Language: Английский

Citations

11
Microbiota-mediated competition between Drosophila species DOI Creative Commons
Antoine Rombaut, Romain Gallet, Kenza Qitout

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Sept. 7, 2023

The influence of microbiota in ecological interactions, and particular competition, is poorly known. We studied competition between two insect species, the invasive pest Drosophila suzukii model melanogaster, whose larval niches overlap ripe, but not rotten, fruit.We discovered D. females prevent costly interspecific by avoiding oviposition on substrates previously visited melanogaster. More precisely, melanogaster association with gut bacteria genus Lactobacillus triggered avoidance. However, avoidance behavior condition-dependent, that themselves carry stop sites adaptive significance cues from competitor's was revealed experimentally reproducing in-fruit competition: reduced survival larvae only occurred if competitor had its normal microbiota.This study establishes microbiotas as potent mediators reveals a central role for context-dependent behaviors under bacterial influence. Video Abstract.

Language: Английский

Citations

4
Insect Lipid Metabolism in the Presence of Symbiotic and Pathogenic Viruses and Bacteria DOI
Bertanne Visser,

Mathilde Scheifler

Advances in experimental medicine and biology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

1
Beyond canonical models: why a broader understanding of Diptera-microbiota interactions is essential for vector-borne disease control DOI
Aldo A. Arellano, Andrew J. Sommer, Kerri L. Coon

et al.

Evolutionary Ecology, Journal Year: 2022, Volume and Issue: 37(1), P. 165 - 188

Published: July 28, 2022

Language: Английский

Citations

6