Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing DOI Creative Commons
Morghan C. Lucas, Leszek P. Pryszcz, Rebeca Medina

и другие.

Nature Biotechnology, Год журнала: 2023, Номер 42(1), С. 72 - 86

Опубликована: Апрель 6, 2023

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult part because simple method to simultaneously quantify their abundance and chemical modifications is lacking. Here we introduce Nano-tRNAseq, nanopore-based approach sequence native tRNA populations that provides quantitative estimates of both abundances modification dynamics single experiment. We show default nanopore sequencing settings discard the vast majority reads, leading poor yields biased representations based on transcript length. Re-processing raw current intensity signals leads 12-fold increase number recovered reads enables recapitulation accurate abundances. then apply Nano-tRNAseq Saccharomyces cerevisiae populations, revealing crosstalks interdependencies between different types within same molecule changes response oxidative stress.

Язык: Английский

Writers, readers and erasers of RNA modifications in cancer DOI Creative Commons
Rosaura Esteve‐Puig, Alberto Bueno-Costa, Manel Esteller

и другие.

Cancer Letters, Год журнала: 2020, Номер 474, С. 127 - 137

Опубликована: Янв. 25, 2020

Although cancer was originally considered a disease driven only by genetic mutations, it has now been proven that is also an epigenetic DNA hypermethylation-associated silencing of tumor suppressor genes and aberrant histone modifications. Very recently, third component emerged: the so-called epitranscriptome understood as chemical modifications RNA regulate alter activity molecules. In this regard, study disruption RNA-modifying proteins gaining momentum in advancing our understanding biology. Furthermore, development epitranscriptomic anticancer drugs could lead to new promising unexpected therapeutic strategies for oncology coming years.

Язык: Английский

Процитировано

141
Epigenetic inheritance of polycystic ovary syndrome — challenges and opportunities for treatment DOI
Elisabet Stener‐Victorin, Qiaolin Deng

Nature Reviews Endocrinology, Год журнала: 2021, Номер 17(9), С. 521 - 533

Опубликована: Июль 7, 2021

Язык: Английский

Процитировано

140
WTAP-mediated m6A modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis DOI Open Access
Zhi‐Xuan Li,

Zi‐Qi Zheng,

Pan-Yang Yang

и другие.

Cell Death and Differentiation, Год журнала: 2022, Номер 29(6), С. 1137 - 1151

Опубликована: Янв. 8, 2022

Язык: Английский

Процитировано

131
RNA editing underlies genetic risk of common inflammatory diseases DOI
Qin Li, Michael J. Gloudemans, Jonathan M. Geisinger

и другие.

Nature, Год журнала: 2022, Номер 608(7923), С. 569 - 577

Опубликована: Авг. 3, 2022

Язык: Английский

Процитировано

119
Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing DOI Creative Commons
Morghan C. Lucas, Leszek P. Pryszcz, Rebeca Medina

и другие.

Nature Biotechnology, Год журнала: 2023, Номер 42(1), С. 72 - 86

Опубликована: Апрель 6, 2023

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult part because simple method to simultaneously quantify their abundance and chemical modifications is lacking. Here we introduce Nano-tRNAseq, nanopore-based approach sequence native tRNA populations that provides quantitative estimates of both abundances modification dynamics single experiment. We show default nanopore sequencing settings discard the vast majority reads, leading poor yields biased representations based on transcript length. Re-processing raw current intensity signals leads 12-fold increase number recovered reads enables recapitulation accurate abundances. then apply Nano-tRNAseq Saccharomyces cerevisiae populations, revealing crosstalks interdependencies between different types within same molecule changes response oxidative stress.

Язык: Английский

Процитировано

113