Direct detection of RNA modifications and structure using single-molecule nanopore sequencing DOI Creative Commons
William Stephenson, Roham Razaghi,

Steven Busan

et al.

Cell Genomics, Journal Year: 2022, Volume and Issue: 2(2), P. 100097 - 100097

Published: Feb. 1, 2022

Modifications are present on many classes of RNA, including tRNA, rRNA, and mRNA. These modifications modulate diverse biological processes such as genetic recoding mRNA export folding. In addition, can be introduced to RNA molecules using chemical probing strategies that reveal structure dynamics. Many methods exist detect by short-read sequencing; however, limitations read length inherent short-read-based dissociate from their native context, preventing single-molecule modification analysis. Here, we demonstrate direct nanopore sequencing endogenous exogenous long RNAs at the level. We 2'-O-methyl base across E. coli S. cerevisiae ribosomal shifts in current signal dwell times distally through interactions with helicase motor protein. further use 2'-hydroxyl reactive SHAPE reagent acetylimidazole probe level readout sequencing.

Language: Английский

Writers, readers and erasers of RNA modifications in cancer DOI Creative Commons
Rosaura Esteve‐Puig, Alberto Bueno-Costa, Manel Esteller

et al.

Cancer Letters, Journal Year: 2020, Volume and Issue: 474, P. 127 - 137

Published: Jan. 25, 2020

Although cancer was originally considered a disease driven only by genetic mutations, it has now been proven that is also an epigenetic DNA hypermethylation-associated silencing of tumor suppressor genes and aberrant histone modifications. Very recently, third component emerged: the so-called epitranscriptome understood as chemical modifications RNA regulate alter activity molecules. In this regard, study disruption RNA-modifying proteins gaining momentum in advancing our understanding biology. Furthermore, development epitranscriptomic anticancer drugs could lead to new promising unexpected therapeutic strategies for oncology coming years.

Language: Английский

Citations

139
Epigenetic inheritance of polycystic ovary syndrome — challenges and opportunities for treatment DOI
Elisabet Stener‐Victorin, Qiaolin Deng

Nature Reviews Endocrinology, Journal Year: 2021, Volume and Issue: 17(9), P. 521 - 533

Published: July 7, 2021

Language: Английский

Citations

135
WTAP-mediated m6A modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis DOI Open Access
Zhi‐Xuan Li,

Zi‐Qi Zheng,

Pan-Yang Yang

et al.

Cell Death and Differentiation, Journal Year: 2022, Volume and Issue: 29(6), P. 1137 - 1151

Published: Jan. 8, 2022

Language: Английский

Citations

125
RNA editing underlies genetic risk of common inflammatory diseases DOI
Qin Li, Michael J. Gloudemans, Jonathan M. Geisinger

et al.

Nature, Journal Year: 2022, Volume and Issue: 608(7923), P. 569 - 577

Published: Aug. 3, 2022

Language: Английский

Citations

119
Direct detection of RNA modifications and structure using single-molecule nanopore sequencing DOI Creative Commons
William Stephenson, Roham Razaghi,

Steven Busan

et al.

Cell Genomics, Journal Year: 2022, Volume and Issue: 2(2), P. 100097 - 100097

Published: Feb. 1, 2022

Modifications are present on many classes of RNA, including tRNA, rRNA, and mRNA. These modifications modulate diverse biological processes such as genetic recoding mRNA export folding. In addition, can be introduced to RNA molecules using chemical probing strategies that reveal structure dynamics. Many methods exist detect by short-read sequencing; however, limitations read length inherent short-read-based dissociate from their native context, preventing single-molecule modification analysis. Here, we demonstrate direct nanopore sequencing endogenous exogenous long RNAs at the level. We 2'-O-methyl base across E. coli S. cerevisiae ribosomal shifts in current signal dwell times distally through interactions with helicase motor protein. further use 2'-hydroxyl reactive SHAPE reagent acetylimidazole probe level readout sequencing.

Language: Английский

Citations

111