Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines
Cellular and Molecular Life Sciences,
Год журнала:
2024,
Номер
81(1)
Опубликована: Окт. 8, 2024
The
establishment
of
epiblast-derived
pluripotent
stem
cells
(PSCs)
from
cattle,
which
are
important
domestic
animals
that
provide
humans
with
milk
and
meat
while
also
serving
as
bioreactors
for
producing
valuable
proteins,
poses
a
challenge
due
to
the
unclear
molecular
signaling
required
embryonic
epiblast
development
maintenance
PSC
self-renewal.
Here,
we
selected
six
key
stages
bovine
embryo
(E5,
E6,
E7,
E10,
E12,
E14)
track
changes
in
pluripotency
dependence
on
pathways
via
modified
single-cell
transcription
sequencing
technology.
remarkable
similarity
gene
expression
patterns
between
cattle
pigs
during
lineage
contributed
successful
(bEpiSCs)
using
3i/LAF
(WNTi,
GSK3βi,
SRCi,
LIF,
Activin
A,
FGF2)
culture
system.
generated
bEpiSCs
exhibited
consistent
formative
genes
maintained
clonal
morphology,
normal
karyotypes,
proliferative
capacity
more
than
112
passages.
Moreover,
these
high-efficiency
teratoma
formation
well
ability
differentiate
into
various
cell
lineages.
potential
myogenic
differentiation,
primordial
germ
like
(PGCLCs)
induction,
donor
nuclear
transfer
was
assessed,
indicating
their
promise
advancing
cell-cultured
production,
editing,
animal
breeding.
Язык: Английский
Tankyrase inhibitor IWR-1 modulates HIPPO and Transforming Growth Factor β signaling in primed bovine embryonic stem cells cultured on mouse embryonic fibroblasts
Theriogenology,
Год журнала:
2024,
Номер
233, С. 100 - 111
Опубликована: Ноя. 19, 2024
Язык: Английский
In Vitro Growth of Mammalian Follicles and Oocytes
Animals,
Год журнала:
2024,
Номер
14(9), С. 1355 - 1355
Опубликована: Апрель 30, 2024
Mammalian
ovaries
contain
a
large
number
of
immature
follicles,
most
which
are
destined
to
degenerate
before
ovulation
[...]
Язык: Английский
Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 30, 2024
Abstract
The
establishment
of
epiblast-derived
pluripotent
stem
cells
(PSCs)
from
cattle,
which
are
important
domestic
animals
that
provide
humans
with
milk
and
meat
while
also
serving
as
bioreactors
for
producing
valuable
proteins,
poses
a
challenge
due
to
the
unclear
molecular
signaling
required
embryonic
epiblast
development
maintenance
PSC
self-renewal.
Here,
we
selected
six
key
stages
bovine
embryo
(E5,
E6,
E7,
E10,
E12,
E14)
track
changes
in
pluripotency
dependence
on
pathways
via
modified
single-cell
transcription
sequencing
technology.
remarkable
similarity
gene
expression
patterns
between
cattle
pigs
during
lineage
contributed
successful
(bEpiSCs)
using
3i/LAF
(WNTi,
GSK3βi,
SRCi,
LIF,
Activin
A,
FGF2)
culture
systems.
generated
bEpiSCs
exhibited
consistent
formative
genes
maintained
clonal
morphology,
normal
karyotypes,
proliferative
capacity
more
than
112
passages.
Moreover,
these
high-efficiency
teratoma
formation
well
ability
differentiate
into
various
cell
lineages.
potential
myogenic
differentiation,
primordial
germ
like
(PGCLCs)
induction
donor
somatic
nuclear
transfer
(SCNT)
was
assessed,
indicating
their
promise
advancing
cell-cultured
production,
editing,
animal
breeding.
Язык: Английский
Livestock embryonic stem cells for reproductive biotechniques and genetic improvement
Animal Reproduction,
Год журнала:
2024,
Номер
21(3)
Опубликована: Янв. 1, 2024
Embryonic
stem
cells
(ESCs)
have
proven
to
be
a
great
Язык: Английский
Use of induced pluripotent stem cells for regenerative medicine and understanding of cell biology
Reproduction Fertility and Development,
Год журнала:
2024,
Номер
37(1)
Опубликована: Дек. 12, 2024
Stem
cells
are
a
highly
desirable
tool
for
regenerative
medicine
due
to
unique
characteristics
such
as
immunomodulation
and
angiogenesis
(multipotent
cells)
high
self-renewal
potential
differentiation
capability
(pluripotent
cells),
thus
being
classified
according
their
stage
of
dedifferentiation
epigenetic
profile.
Apart
from
used
in
vitro
disease
modeling
or
even
vivo
therapies,
pluripotent
stem
valuable
animal
production
breeding
improvement.
In
particular,
the
lack
robustness
ethical
concerns
regarding
embryonic
cells,
induced
(iPSCs)
emerge
new
‘game
changer’
veterinary
translational
medicine.
Herein,
we
present
discuss
recent
uses
understanding
cell
biology,
focusing
on
generating
using
iPSCs
diverse
species
aiming
genetic
conservation
dissemination
gametogenesis,
cellular
agriculture.
Язык: Английский
What we can learn from the bovine embryo and mouse models to enable in vitro gametogenesis in cattle
Reproduction Fertility and Development,
Год журнала:
2024,
Номер
37(1)
Опубликована: Дек. 12, 2024
The
development
of
in
vitro
gametogenesis
(IVG)
the
mouse
opened
up
unforeseen
possibilities
for
assisted
reproduction.
this
technology
to
be
used
cattle
production
could
accelerate
rate
genetic
selection
by
dramatically
reducing
generation
interval,
while
decreasing
environmental
impact
livestock
as
need
grow
animals
process
would
reduced
or
even
eliminated.
Although
several
steps
IVG
such
oocyte
maturation
and
fertilization,
embryo
are
already
routinely
performed
cattle,
other
system
follicle
still
rudimentary.
stable
derivation
bovine
pluripotent
stem
cells
is
starting
point
without
which
cannot
realized.
However,
producing
a
primordial
germ
cell
taking
through
oogenesis
folliculogenesis
dish
will
require
more
detailed
understanding
milestones
that
accomplished
vivo
before
they
can
recapitulated
vitro.
In
particular,
regulatory
circuitry
specification
embryo,
timing
events
related
program,
factors
necessary
make
competent
egg,
uncovered.
Here,
we
review
provide
brief
description
current
advances
bottlenecks
cattle.
Finally,
comparison
between
mice
cows
regard.
Язык: Английский