Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

ACS Nano, Год журнала: 2022, Номер 16(8), С. 11516 - 11544

Опубликована: Авг. 2, 2022

Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural shift of light as it passes through transparent object, such mammalian cell, to quantify biomass distribution and spatial temporal changes in biomass. Reported cell studies more than 60 years ago, ongoing advances hardware software are leading numerous applications biology, dramatic expansion utility over past two decades. Today, investigations size, morphology, behavior, cellular viscoelasticity, drug efficacy, accumulation turnover, transport mechanics supporting development, physiology, neural activity, cancer, additional physiological processes diseases. Here, we review field biology starting underlying principles, followed by discussion technical approaches currently available or being developed, end an examination breadth use under development. We comment on strengths shortcomings deployment key contexts conclude emerging challenges based combining other methodologies that expand scope even further.

Язык: Английский

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Roadmap on wavefront shaping and deep imaging in complex media

Journal of Physics Photonics, Год журнала: 2022, Номер 4(4), С. 042501 - 042501

Опубликована: Июнь 8, 2022

The last decade has seen the development of a wide set tools, such as wavefront shaping, computational or fundamental methods, that allow to understand and control light propagation in complex medium, biological tissues multimode fibers. A vibrant diverse community is now working on this field, revolutionized prospect diffraction-limited imaging at depth tissues. This roadmap highlights several key aspects fast developing some challenges opportunities ahead.

Язык: Английский

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Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy

Light Science & Applications, Год журнала: 2022, Номер 11(1)

Опубликована: Июнь 1, 2022

We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution biological specimens from intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative phase-contrast imaging. The unique combination z-scanning sample angle diversity in provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution thick samples. Based on an off-the-shelf bright-field microscope programmable light-emitting-diode (LED) source, achieves imaging resolution 206 nm laterally 520 axially high-NA oil immersion objective. validate RI tomographic performance unlabeled fixed live samples, including human breast cancer cell lines MCF-7, hepatocyte carcinoma HepG2, mouse macrophage RAW 264.7, Caenorhabditis elegans (C. elegans), Henrietta Lacks (HeLa) cells. These results establish as approach microscopy, permitting characterization morphology time-dependent subcellular changes widespread medical applications.

Язык: Английский

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Quantitative phase imaging based on holography: trends and new perspectives

Light Science & Applications, Год журнала: 2024, Номер 13(1)

Опубликована: Июнь 27, 2024

Abstract In 1948, Dennis Gabor proposed the concept of holography, providing a pioneering solution to quantitative description optical wavefront. After 75 years development, holographic imaging has become powerful tool for wavefront measurement and phase imaging. The emergence this technology given fresh energy physics, biology, materials science. Digital holography (DH) possesses advantages wide-field, non-contact, precise, dynamic capability complex-waves. DH unique capabilities propagation fields by measuring light scattering with information. It offers visualization refractive index thickness distribution weak absorption samples, which plays vital role in pathophysiology various diseases characterization materials. provides possibility bridge gap between disciplines. is described complex amplitude. complex-value complex-domain reconstructed from intensity-value camera real-domain. Here, we regard process recording reconstruction as transformation real-domain, discuss mathematics physical principles reconstruction. We review underlying principles, technical approaches, breadth applications. conclude emerging challenges opportunities based on combining other methodologies that expand scope utility even further. multidisciplinary nature brings application experts together label-free cell analytical chemistry, clinical sciences, sensing, semiconductor production.

Язык: Английский

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Towards in-vivo label-free detection of brain tumor margins with epi-illumination tomographic quantitative phase imaging

Biomedical Optics Express, Год журнала: 2021, Номер 12(3), С. 1621 - 1621

Опубликована: Фев. 20, 2021

Brain tumor surgery involves a delicate balance between maximizing the extent of resection while minimizing damage to healthy brain tissue that is vital for neurological function. However, differentiating tumor, particularly infiltrative disease, and in-vivo remains significant clinical challenge. Here we demonstrate quantitative oblique back illumination microscopy (qOBM)—a novel label-free optical imaging technique achieves tomographic phase in thick scattering samples—clearly differentiates including disease. Data from bulk animal model show qOBM enables fresh tissues with remarkable cellular subcellular detail closely resembles histopathology using hematoxylin eosin (H&E) stained fixed sections, gold standard cancer detection. Quantitative biophysical features are also extracted which yield robust surrogate biomarkers disease enable (1) automated margin detection high sensitivity specificity (2) facile visualization regions. Finally, develop low-cost, flexible, fiber-based handheld device brings this technology one step closer use. This work has implications guiding neurosurgery by paving way tool delivers real-time, label-free,

Язык: Английский

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Bond-selective intensity diffraction tomography

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Дек. 15, 2022

Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity fingerprint extraction is validated. Volumetric chemical of biological cells demonstrated at speed ~20 s per volume, lateral axial resolution ~350 nm ~1.1 µm, respectively. BS-IDT's application potential investigated by chemically quantifying lipids stored cancer volumetric Caenorhabditis elegans large field view (~100 µm x 100 µm).

Язык: Английский

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Measurement of tissue optical properties in a wide spectral range: a review [Invited]

Biomedical Optics Express, Год журнала: 2022, Номер 14(1), С. 249 - 249

Опубликована: Дек. 5, 2022

A distinctive feature of this review is a critical analysis methods and results measurements the optical properties tissues in wide spectral range from deep UV to terahertz waves. Much attention paid refractive index biological liquids, knowledge which necessary for effective application many imaging diagnostics. The parameters healthy pathological are presented, reasons their differences discussed, important discrimination pathologies demarcation boundaries. When considering interaction radiation with tissues, concept an medium relaxation models presented. Attention drawn manifestation scattering THz problems measuring discussed. In conclusion, method dynamic under clearing using immersion agents main mechanisms technologies clearing, as well examples successful differentiation analyzed.

Язык: Английский

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Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual hematoxylin and eosin staining

Optica, Год журнала: 2023, Номер 10(12), С. 1605 - 1605

Опубликована: Окт. 25, 2023

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis comprehensive clinical assessment tissue. However, typical formalin-fixation, paraffin-embedding (FFPE) process is laborious time consuming, often limiting its usage in time-sensitive applications such surgical margin assessment. To address these challenges, we combine an emerging 3D quantitative phase imaging technology, termed oblique back illumination microscopy (qOBM), with unsupervised generative adversarial network pipeline to map qOBM images unaltered thick tissues (i.e., label- slide-free) virtually stained H&E-like (vH&E) images. We demonstrate that approach achieves high-fidelity conversions H&E subcellular detail using fresh specimens from mouse liver, rat gliosarcoma, human gliomas. also show framework directly enables additional capabilities contrast volumetric imaging. The quality fidelity vH&E are validated both a neural classifier trained on real tested virtual images, user study neuropathologists. Given simple low-cost embodiment ability provide real-time feedback

Язык: Английский

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k-space holographic multiplexing for synthetic aperture diffraction tomography

APL Photonics, Год журнала: 2024, Номер 9(5)

Опубликована: Май 1, 2024

Optical diffraction tomography can be performed with low phototoxicity and photobleaching to analyze 3D cells tissues. It is desired develop high throughput powerful data processing capabilities. We propose bandwidth holographic microscopy (HBHM). Based on the analyticity of complex amplitudes, unified multiplexing transfer function established. A scattering field achieved via k-space optical origami two 2D wavefronts from one interferogram. Scanning illumination modulates high-horizontal axial endow synthetic-aperture space-bandwidth product (SBP) fields. The bright-field counterpart SBP a single HBHM 14.6 megapixels, while number pixels only 13.7 megapixels. achieves an eight-fold enhancement under same limit. paves way toward performance throughput, large-scale, non-invasive histopathology, cell biology, industrial inspection.

Язык: Английский

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In-silico clearing approach for deep refractive index tomography by partial reconstruction and wave-backpropagation

Light Science & Applications, Год журнала: 2023, Номер 12(1)

Опубликована: Апрель 27, 2023

Refractive index (RI) is considered to be a fundamental physical and biophysical parameter in biological imaging, as it governs light-matter interactions light propagation while reflecting cellular properties. RI tomography enables volumetric visualization of distribution, allowing biologically relevant analysis sample. However, multiple scattering (MS) sample-induced aberration (SIA) caused by the inhomogeneity distribution thick sample make its challenging. This paper proposes deep tomographic approach overcome MS SIA allow enhanced reconstruction samples compared that enabled conventional linear-model-based tomography. The proposed consists partial using holograms acquired with angular diversity their backpropagation reconstructed map, which unambiguously reconstructs next volume. Repeating this operation efficiently entire tomogram suppressing SIA. We visualized multicellular spheroid diameter 140 µm within minutes reconstruction, thereby demonstrating capability computational efficiency method those Furthermore, we quantified high-RI structures morphological changes inside spheroids, indicating can retrieve information from distribution. Benefitting excellent interpretability distributions, label-free facilitates noninvasive understanding architecture time-course specimens.

Язык: Английский

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