Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Авг. 14, 2023

Abstract Microorganisms play essential roles in the health and resilience of cnidarians. Understanding factors influencing cnidarian microbiomes requires cross study comparisons, yet plethora protocols used hampers dataset integration. We unify 16S rRNA gene sequences from microbiome studies under a single analysis pipeline. reprocess 12,010 samples 186 studies, alongside 3,388 poriferan, 370 seawater samples, 245 cultured Symbiodiniaceae, unifying ~6.5 billion sequence reads. Samples are partitioned by hypervariable region sequencing platform to reduce variability. This systematic review uncovers an incredible diversity 86 archaeal bacterial phyla associated with Cnidaria, highlights key bacteria hosted across host sub-phylum, depth, microhabitat. Shallow (< 30 m) water Alcyonacea Actinaria characterized highly shared relatively abundant microbial communities, unlike Scleractinia most deeper Utilizing V4 region, we find that composition, richness, diversity, structure primarily influenced phylogeny, sampling ocean body, followed microhabitat date. identify geographical generalist specific Endozoicomonas clades within Cnidaria Porifera. forms framework for understanding governing creates baseline assessing stress dysbiosis.

Язык: Английский

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A review of quorum-sensing and its role in mediating interkingdom interactions in the ocean

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Фев. 5, 2025

Quorum sensing, first described in marine systems five decades ago, is a well-characterized chemical communication system used to coordinate bacterial gene expression and behavior; however, the impact of quorum sensing on interkingdom interactions has been vastly understudied. In this review, we examine how these molecules mediate between bacteria eukaryotes; influencing processes such as development, disease pathogenesis, microbiome regulation within ecosystems. We describe varied mechanisms eukaryotes have evolved interfere with signaling, crucial role signals play host-virus interactions, their exchange may be governed by outer membrane vesicles, prevalent systems. Here, present dynamic portrayal beyond communication, laying groundwork for future investigations roles shaping ecosystem structure function. A review examines system, shapes eukaryote

Язык: Английский

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The biogeographic differentiation of algal microbiomes in the upper ocean from pole to pole

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Сен. 16, 2021

Eukaryotic phytoplankton are responsible for at least 20% of annual global carbon fixation. Their diversity and activity shaped by interactions with prokaryotes as part complex microbiomes. Although differences in their local species have been estimated, we still a limited understanding environmental conditions compositional between communities on large scale from pole to pole. Here, show, based pole-to-pole metatranscriptomes microbial rDNA sequencing, that polar non-polar upper oceans most strongly impact the large-scale spatial pattern biodiversity gene algal The geographic differentiation co-occurring microbes microbiomes can be well explained latitudinal temperature gradient associated break points beta diversity, an average breakpoint 14 °C ± 4.3, separating cold warm oceans. As warming impacts ocean temperatures, project move markedly pole-wards. Hence, abrupt regime shifts could caused anthropogenic climate change.

Язык: Английский

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Host genetics, phenotype and geography structure the microbiome of a foundational seaweed

Molecular Ecology, Год журнала: 2022, Номер 31(7), С. 2189 - 2206

Опубликована: Фев. 1, 2022

Interactions between hosts and their microbiota are vital to the functioning resilience of macro-organisms. Critically, for that play foundational roles in communities, understanding what drives host-microbiota interactions is essential informing ecosystem restoration conservation. We investigated relative influence host traits surrounding environment on microbial communities associated with seaweed Phyllospora comosa. quantified 16 morphological functional phenotypic traits, including genetics (using 354 single nucleotide polymorphisms) surface-associated 16S rRNA gene amplicon sequencing) from 160 individuals sampled eight sites spanning Phyllospora's entire latitudinal distribution (1,300 km). Combined, these factors explained 54% overall variation community structure, much which was related local (~32%). found putative "core" taxa (i.e., present all sampled) exhibited slightly higher associations when compared "variable" (not individuals). identified several key genetic loci were strongly multiple sequence variants, known defence, disease tissue degradation. This information how host-associated vary enhances our current "holobionts" (hosts plus microbiota) structured. Such can be used inform management strategies important vulnerable habitats.

Язык: Английский

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Seasonal dynamics of the microbiome-host response to pharmaceuticals and pesticides in Mytilus galloprovincialis farmed in the Northwestern Adriatic Sea

The Science of The Total Environment, Год журнала: 2023, Номер 887, С. 163948 - 163948

Опубликована: Май 5, 2023

Marine mussels, especially Mytilus galloprovincialis, are well-established sentinel species, being naturally resistant to the exposure multiple xenobiotics of natural and anthropogenic origin. Even if response xenobiotic is well known at host level, role mussel-associated microbiome in animal environmental pollution poorly explored, despite its potential detoxification important development, protection, adaptation. Here, we characterized microbiome-host integrative M. galloprovincialis a real-world setting, involving complex pattern emerging pollutants, as occurs Northwestern Adriatic Sea. A total 387 mussel individuals from 3 commercial farms, spanning about 200 km along coast, different seasons, were collected. Multiresidue analysis (for quantitative determination), transcriptomics physiological response), metagenomics host-associated microbial taxonomical functional features) analyses performed on digestive glands. According our findings, responds presence pollutants - including antibiotics sulfamethoxazole, erythromycin, tetracycline, herbicides atrazine metolachlor, insecticide N,N-diethyl-m-toluamide integrating defense mechanisms, e.g., through upregulation transcripts involved metabolic activity, microbiome-mediated functions, functionalities multidrug or tetracycline resistance. Overall, data highlight importance strategic player for orchestration resistance multixenobiotic holobiont providing substances, occurring real world settings. Complementing with microbiome-dependent degradative genes, gland associated can have an context high pressure, supporting relevance systems animal-based bioremediation tool.

Язык: Английский

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Manipulation of the seagrass‐associated microbiome reduces disease severity

Environmental Microbiology, Год журнала: 2024, Номер 26(2)

Опубликована: Янв. 9, 2024

Abstract Host‐associated microbes influence host health and function can be a first line of defence against infections. While research increasingly shows that terrestrial plant microbiomes contribute to bacterial, fungal, oomycete disease resistance, no comparable experimental work has investigated marine or more diverse agents. We test the hypothesis eelgrass ( Zostera marina ) leaf microbiome increases resistance seagrass wasting disease. From field with paired diseased asymptomatic tissue, 16S rRNA gene amplicon sequencing revealed bacterial composition richness varied markedly between tissue in one two years. This suggests on microbial communities may vary environmental conditions. next experimentally reduced antibiotics bleach, then inoculated plants Labyrinthula zosterae , causative agent detected significantly higher severity native than an microbiome. Our results over multiple experiments do not support protective role L. . Further studies these host–microbe–pathogen relationships continue show new diseases.

Язык: Английский

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Effects of temperature and microbial disruption on juvenile kelp Ecklonia radiata and its associated bacterial community

Frontiers in Marine Science, Год журнала: 2024, Номер 10

Опубликована: Янв. 18, 2024

Ocean warming can affect the development and physiological responses of kelps, under future climate change scenarios, increasing seawater temperatures pose a major threat to these habitat-forming species. However, little is known about effects on epiphytic bacterial communities how an altered microbiome may interact with temperature stress, affecting condition survival kelp, particularly potentially more vulnerable early life stages. Here, we tested thermal stress growth juvenile kelp Ecklonia radiata in which their community was experimentally disrupted using antimicrobials, simulating dysbiosis. We hypothesized that, (23°C, extreme scenario ocean Tasmania), would be strongly affected than undisrupted or ambient (14°C) but microbiota. Thermal reduced growth, increased tissue bleaching negatively net photosynthesis kelp. In addition, substantial structure also found conditions, increase abundance pathogenic groups. microbial disruption did not act synergistically juveniles. These results suggest that elevated kelps microbially-mediated juveniles less susceptible disruptions microbiome.

Язык: Английский

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Differential responses of selectively bred mussels (Perna canaliculus) to heat stress—survival, immunology, gene expression and microbiome diversity

Frontiers in Physiology, Год журнала: 2024, Номер 14

Опубликована: Фев. 15, 2024

New Zealand’s green-lipped mussel ( Perna canaliculus ) is an ecologically and economically important species. Marine heatwaves are increasing in frequency around NZ’s coastline, these events correlated with increased stress mortality of some aquaculture This study aimed to identify general biomarkers heat P. assess whether responses differed between genetically distinct selectively bred mussels. We exposed three families mussels (families A, B C) seawater temperature regimes the laboratory: 1) a “control” treatment (ambient 12°C), 2) 26°C challenge subsequent recovery period, 3) sustained no recovery. investigated survival, immune response (hemocyte concentration viability, oxidative total antioxidant capacity), hemocyte gene expression gill microbiome during challenges. In heat-stress treatment, family A had highest survival rate (42% compared 25% 5% for C B, respectively). Gene levels significantly shifted thermal families, more dissimilar than C. Family substantially genes impacted by timepoint other while very little genes/pathways that responded stress. Genes related shock proteins (e.g., AIF1, CTSC, TOLL8, CASP9, FNTA, AHCY, CRYAB, PPIF) were upregulated all Microbiome species-richness before heat-stress, having distinctly different flora families. Microbial diversity changed similarly prolonged species Vibrio Campylobacter Our highlights use non-lethal sampling hemocytes as diagnostic tool explore mussels, predict their ocean warming. approach can potential thermotolerant candidates further selective breeding, which may increase resilience industry warming ocean.

Язык: Английский

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The Seagrass Holobiont: What We Know and What We Still Need to Disclose for Its Possible Use as an Ecological Indicator

Water, Год журнала: 2021, Номер 13(4), С. 406 - 406

Опубликована: Фев. 4, 2021

Microbes and seagrass establish symbiotic relationships constituting a functional unit called the holobiont that reacts as whole to environmental changes. Recent studies have shown microbial associated community varies according host species, conditions host’s health status, suggesting communities respond rapidly disturbances These changes, dynamics of which are still far from being clear, could represent sensitive monitoring tool ecological indicator detect early stages stress. In this review, state art on is discussed in perspective, with aim disentangling influence different factors shaping it. As an example, we expand widely studied Halophila stipulacea’s community, highlighting changing constant components microbes, conditions. pivotal contribution understanding holobiont’s variability pattern, potential development ecological/ecotoxicological indices. The influences physiological status holobiont, alongside bioinformatic tools for data analysis, key topics need be deepened, order use seagrass-microbial interactions source information.

Язык: Английский

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Newly formed riparian microhabitats simplify bacterial community structure and diversity

Journal of Soils and Sediments, Год журнала: 2023, Номер 23(4), С. 1927 - 1943

Опубликована: Фев. 9, 2023

Язык: Английский

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