Enhanced lipid metabolism reprogramming in CHF rats through IL-6-mediated cardiac glial cell modulation by digilanid C and electroacupuncture stimulation combination DOI Creative Commons
Yun Liu, Xiao Sun,

Mingqian Yuan

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Сен. 3, 2024

Background Cardiac lipid metabolism reprogramming is recognized as a critical pathological factor in the progression of chronic heart failure (CHF). The therapeutic potential digilanid C and electroacupuncture stimulation (ES) enhancing cardiac function has been established. However, optimal synergistic regulatory strategies these interventions on have yet to be elucidated. Methods This study aimed comprehensively evaluate impact C-ES combination steatosis remodeling CHF. Assessments were conducted across various dimensions, including myocardial oxygen consumption, mitochondrial function, metabolism. Additionally, we sought uncover underlying neuromolecular mechanisms. Results Our findings, at both molecular morphological levels, indicated that application ES significantly inhibited fibrosis steatosis. therapy facilitated repair neuro-vascular uncoupling induced Notably, ameliorated cardiomyocyte apoptosis enhanced biogenesis CHF, leading restructured energy supply pattern. immunofluorescence analyses revealed aggregation glial cells (CGCs) sites abnormal neurovascular coupling, response degeneration. was accompanied by marked reduction abnormally elevated expression interleukin 6 (IL-6) glutamatergic signaling, which correlated with severity aberrant activation CGCs. combined found activate Janus kinase 1 (JAK1)/signal transducer activator transcription 3 (STAT3) pathway, effectively attenuated accumulation over-recruitment CGCs deprivation nerves. Conclusion These findings underscore novel approach modulate complex interplay between dynamics metabolic dysregulation

Язык: Английский

Spatially resolved multiomics of human cardiac niches DOI Creative Commons
Kazumasa Kanemaru, James Cranley, Daniele Muraro

и другие.

Nature, Год журнала: 2023, Номер 619(7971), С. 801 - 810

Опубликована: Июль 12, 2023

The function of a cell is defined by its intrinsic characteristics and niche: the tissue microenvironment in which it dwells. Here we combine single-cell spatial transcriptomics data to discover cellular niches within eight regions human heart. We map cells microanatomical locations integrate knowledge-based unsupervised structural annotations. also profile cardiac conduction system

Язык: Английский

Процитировано

170
Impact of Pulsed-Field Ablation on Intrinsic Cardiac Autonomic Nervous System After Pulmonary Vein Isolation DOI Creative Commons
Shota Tohoku, Boris Schmidt, David Schaack

и другие.

JACC. Clinical electrophysiology, Год журнала: 2023, Номер 9(9), С. 1864 - 1875

Опубликована: Июль 19, 2023

Язык: Английский

Процитировано

29
Revisiting Cardiac Biology in the Era of Single Cell and Spatial Omics DOI
Jack A. Palmer, Nadia Rosenthal, Sarah A. Teichmann

и другие.

Circulation Research, Год журнала: 2024, Номер 134(12), С. 1681 - 1702

Опубликована: Июнь 6, 2024

Throughout our lifetime, each beat of the heart requires coordinated action multiple cardiac cell types. Understanding biology, its intricate microenvironments, and mechanisms that govern their function in health disease are crucial to designing novel therapeutical behavioral interventions. Recent advances single-cell spatial omics technologies have significantly propelled this understanding, offering insights into cellular diversity complex interactions tissue. This review provides a comprehensive overview landscape heart, bridging gap between suspension-based emerging situ approaches, focusing on experimental computational challenges, comparative analyses mouse human systems, rising contextualization cells within niches. As we explore at unprecedented resolution, integrating from both studies will pave way for diagnostic tools therapeutic interventions, ultimately improving outcomes patients with cardiovascular diseases.

Язык: Английский

Процитировано

7
The Heart’s Pacemaker Mimics Brain Cytoarchitecture and Function DOI Creative Commons

Rostislav Bychkov,

Magdalena Juhaszova, Miguel Calvo‐Rubio

и другие.

JACC. Clinical electrophysiology, Год журнала: 2022, Номер 8(10), С. 1191 - 1215

Опубликована: Сен. 28, 2022

The sinoatrial node (SAN) of the heart produces rhythmic action potentials, generated via calcium signaling within and among pacemaker cells. Our previous work has described SAN as composed a hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4)-expressing cell meshwork, which merges with network connexin 43+/F-actin+ It is also known that sympathetic parasympathetic innervation create an autonomic plexus in modulates rate rhythm. However, anatomical details interaction this meshwork have yet to be described. This study sought describe 3-dimensional cytoarchitecture mouse SAN, including innervation, peripheral glial cells, whole-mount preparations was examined by three-dimensional confocal laser-scanning microscopy triple immunolabeled combinations antibodies for HCN4, S100 calcium-binding protein B (S100B), fibrillary acidic (GFAP), choline acetyltransferase, or vesicular acetylcholine transporter, tyrosine hydroxylase, transmission electron microscopy. exhibited heterogeneous accompanied web cells novel S100B+/GFAP– interstitial population, unique morphology distinct distribution pattern, creating complex interactions other types node, particularly HCN4-expressing Transmission identified similar population telocytes, appeared secrete vesicles toward Application S100B desynchronized Ca2+ increased variability impulse plexus, web, type embedded HCN4+ increase structural functional complexity provide new regulatory pathway rhythmogenesis.

Язык: Английский

Процитировано

27
What makes the sinoatrial node tick? A question not for the faint of heart DOI Creative Commons
Lorenzo A. H. Donald, Edward G. Lakatta

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2023, Номер 378(1879)

Опубликована: Май 1, 2023

Even before the sinoatrial node (SAN) was discovered, cardiovascular science engaged in an active investigation of when and why heart would beat. After electrochemical theory bioelectric membrane potentials formulated first action were measured contracting muscle cells, field became divided: some investigators studied electrophysiology ion channels, others contraction. It later known that changes intracellular Ca 2+ cause The pacemaking reunited by coupled-clock pacemaker cell function, which integrated cycling transmembrane voltage into one rhythmogenic system. In this review, we will discuss recent discoveries contextualize system, described isolated SAN complex world tissue: heterogeneous local releases, generated within cells regulated other types cytoarchitecture, variably co-localize synchronize to give rise relatively rhythmic impulses emanate from excite heart. We ultimately conceptualize as a brain-like structure, composed intercommunicating meshworks multiple interstitial intertwined networks nerves glial more. This article is part theme issue ‘The heartbeat: its molecular basis physiological mechanisms’.

Язык: Английский

Процитировано

13
The embryonic zebrafish brain is seeded by a lymphatic-dependent population of mrc1+ microglia precursors DOI
Lauren A. Green, Michael R. O’Dea,

Camden A. Hoover

и другие.

Nature Neuroscience, Год журнала: 2022, Номер 25(7), С. 849 - 864

Опубликована: Июнь 16, 2022

Язык: Английский

Процитировано

19
Mechanisms underlying the role of ankyrin-B in cardiac and neurological health and disease DOI Creative Commons

Nicole York,

Juan C. Sanchez-Arias,

Alexa C. H. McAdam

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2022, Номер 9

Опубликована: Авг. 4, 2022

The ANK2 gene encodes for ankyrin-B (ANKB), one of 3 members the ankyrin family proteins, whose name is derived from Greek word anchor. ANKB was originally identified in brain (B denotes “brain”) but has become most widely known its role cardiomyocytes as a scaffolding protein ion channels and transporters, well an interacting structural signaling proteins. Certain loss-of-function variants are associated with primarily cardiac-presenting autosomal-dominant condition incomplete penetrance variable expressivity characterized by predisposition to supraventricular ventricular arrhythmias, arrhythmogenic cardiomyopathy, congenital adult-onset heart disease, sudden death. Another independent group increased risk distinct neurological phenotypes, including epilepsy autism spectrum disorders. mechanisms underlying ANKB's roles cells health disease not fully understood; however, several clues range molecular cell biological studies have emerged. Notably, exhibits isoforms that different cell-type–, tissue–, developmental stage– expression profiles. Given conservation within ankyrins across evolution, model organism enabled discovery could shed important light on protein-protein interactions related regulation cellular polarity, organization, calcium homeostasis, glucose fat metabolism. Along this accumulation evidence suggesting diversity functions, there on-going debate disease. We currently limited understanding how these functions link risk. To end, review will examine potential contribution functional presentation. This highlight impact dysfunction cardiac neuronal significance

Язык: Английский

Процитировано

15
Glial cell activity in cardiovascular diseases and risk of acute myocardial infarction DOI
Nina Truter, Leoné Malan, M. Faadiel Essop

и другие.

AJP Heart and Circulatory Physiology, Год журнала: 2023, Номер 324(4), С. H373 - H390

Опубликована: Янв. 20, 2023

Growing evidence indicates that the pathophysiological link between brain and heart underlies cardiovascular diseases, specifically acute myocardial infarction (AMI). Astrocytes are most abundant glial cells in central nervous system provide support/protection for neurons. peripheral emerging as key modulators of brain-heart axis AMI, by affecting sympathetic activity (centrally peripherally). This review, therefore, aimed to gain an improved understanding cell AMI risk. includes discussions on potential role contributing factors risk, i.e., autonomic dysfunction, glial-neurotrophic ischemic risk markers [glial line-derived neurotrophic factor (GDNF), astrocytic S100 calcium-binding protein B (S100B), silent ischemia, cardiac troponin T (cTnT)]. Consideration related certain disorders, namely, blood-brain barrier chronic psychological stress, may improve our regarding pathological dysfunction can play development/onset AMI. Here, findings demonstrated perturbations (especially activity). Moreover, included sympathovagal imbalance, low GDNF levels reflecting prothrombic hypertension, increased ischemia due perfusion deficits (indicated S100B cTnT levels). Such impacted blood-barrier function were exacerbated during stress. Thus, greater insights consideration such biomarkers help drive future studies investigating pathologies a deeper contributions unlock novel therapies

Язык: Английский

Процитировано

9
Peripheral nervous system glia in support of metabolic tissue functions DOI Creative Commons

Gilian Gunsch,

Emma Paradie,

Kristy L. Townsend

и другие.

Trends in Endocrinology and Metabolism, Год журнала: 2023, Номер 34(10), С. 622 - 639

Опубликована: Авг. 15, 2023

Язык: Английский

Процитировано

9
Microglia cannibalism and efferocytosis leads to shorter lifespans of developmental microglia DOI Creative Commons
Hannah Gordon, Zachary T. Schafer, Cody J. Smith

и другие.

PLoS Biology, Год журнала: 2024, Номер 22(10), С. e3002819 - e3002819

Опубликована: Окт. 30, 2024

The overproduction of cells and subsequent production debris is a universal principle neurodevelopment. Here, we show an additional feature the developing nervous system that causes neural debris—promoted by sacrificial nature embryonic microglia irreversibly become phagocytic after clearing other debris. Described as long-lived, colonize brain persist into adulthood. Using transgenic zebrafish to investigate during construction, identified unlike cell types die in developmental stages they have expanded, necroptosis-dependent microglial prevalent when are expanding brain. Time-lapse imaging demonstrates this cannibalized microglia. To features promote death cannibalism, used time-lapse fate-mapping strategies track lifespan individual These approaches revealed instead being long-lived completely digest their debris, once most eventually die, including ones cannibalistic. results establish paradox—which tested increasing manipulating phagocytosis—that embryo phagocytic, create then microglia, resulting more destined die.

Язык: Английский

Процитировано

3